Search

The role of efficient fuel mixing and a stable flame holder is crucial in enhancing the performance and capabilities of scramjet engines for high-speed flight. The present research paper has tried to disclose the fuel mixing efficiency of 3-lobe annular nozzle on the mixing mechanism of the fuel jet behind the strut. In addition, using internal air jet flow for increasing the circulation strength and fuel mixing behind the strut is also examined in this study. Numerical simulation of the flow and fuel jet behind the strut is done to reveal the main physics related to the mechanism of fuel mixing inside the combustor with the proposed injection system. The results of our simulation show that using annular 3-lobe fuel jet improve the fuel mixing via production of the multiple vortex pairs within the combustor behind the strut. The use of internal air jet also enhances the fuel mixing efficiency up to 90% in combustor of scramjet engine., (© 2024. The Author(s).)

In this study, we present a comprehensive optimization framework employing the Multi-Objective Multi-Verse Optimization (MOMVO) algorithm for the optimal integration of Distributed Generations (DGs) and Capacitor Banks (CBs) into electrical distribution networks. Designed with the dual objectives of minimizing energy losses and voltage deviations, this framework significantly enhances the operational efficiency and reliability of the network. Rigorous simulations on the standard IEEE 33-bus and IEEE 69-bus test systems underscore the effectiveness of the MOMVO algorithm, demonstrating up to a 47% reduction in energy losses and up to a 55% improvement in voltage stability. Comparative analysis highlights MOMVO's superiority in terms of convergence speed and solution quality over leading algorithms such as the Multi-Objective Jellyfish Search (MOJS), Multi-Objective Flower Pollination Algorithm (MOFPA), and Multi-Objective Lichtenberg Algorithm (MOLA). The efficacy of the study is particularly evident in the identification of the best compromise solutions using MOMVO. For the IEEE 33 network, the application of MOMVO led to a significant 47.58% reduction in daily energy loss and enhanced voltage profile stability from 0.89 to 0.94 pu. Additionally, it realized a 36.97% decrease in the annual cost of energy losses, highlighting substantial economic benefits. For the larger IEEE 69 network, MOMVO achieved a remarkable 50.15% reduction in energy loss and improved voltage profiles from 0.89 to 0.93 pu, accompanied by a 47.59% reduction in the annual cost of energy losses. These results not only confirm the robustness of the MOMVO algorithm in optimizing technical and economic efficiencies but also underline the potential of advanced optimization techniques in facilitating the sustainable integration of renewable energy resources into existing power infrastructures. This research significantly contributes to the field of electrical distribution network optimization, paving the way for future advancements in renewable energy integration and optimization techniques for enhanced system efficiency, reliability, and sustainability., (© 2024. The Author(s).)

The effect of temperature on electrochemical properties of Ni 82.3 Cr 7 Fe 3 Si 4.5 B 3.2  glassy alloy in different acid proticity has been investigated utilizing AC and DC methods. Firstly, the handling of experimental data on the temperature dependence of charge transfer resistance, as well as corrosion current density permits us to determine the values of classical Arrhenius parameters as well as the thermodynamic ones considered approximately independent of temperature. This leads us to deduce a global interpretation on the phenomenon of corrosion and polarization. Secondly, the deviation to the linearity of the Arrhenius behavior and the real dependence on temperature of the thermodynamic parameters, permit us to clearly quantify the effect of the acid proticity and define, for the first time, the concept of current Arrhenius parameters and the current thermodynamic ones, as well as the modeling of the enthalpy-enthalpy compensation. Moreover, the effect of temperature can be investigated using the Vogel-Fulcher-Tammann model to reveal that the corresponding Vogel temperature has an interesting physical meaning., (© 2024. The Author(s).)

Xeroderma pigmentosum (XP) is a DNA repair disease that predisposes to early skin cancers as cutaneous melanoma. Melanoma microenvironment contains inflammatory mediators, which would be interesting biomarkers for the prognosis or for the identification of novel therapeutic targets. We used a PCR array to evaluate the transcriptional pattern of 84 inflammatory genes in melanoma tumors obtained from XP patients (XP-Mel) and in sporadic melanoma (SP-Mel) compared to healthy skin. Commonly expressed inflammatory genes were further explored via GTEx and GEPIA databases. The differentially expressed inflammatory genes in XP were compared to their expression in skin exposed to UVs, and evaluated on the basis of the overall survival outcomes of patients with melanoma. Monocyte subsets of patients with SP-Mel, XP and healthy donors were also assessed. PCR array data revealed that 34 inflammatory genes were under-expressed in XP-Mel compared to SP-Mel. Differentially expressed genes that were common in XP-Mel and SP-Mel were correlated with the transcriptomic datasets from GEPIA and GTEx and highlighted the implication of KLK1 and IL8 in the tumorigenesis. We showed also that in XP-Mel tumors, there was an overexpression of KLK6 and KLK10 genes, which seems to be associated with a bad survival rate. As for the innate immunity, we observed a decrease of intermediate monocytes in patients with SP-Mel and in XP. We highlight an alteration in the immune response in XP patients. We identified candidate biomarkers involved in the tumorigenesis, and in the survival of patients with melanoma. Intermediate monocyte's in patients at risk could be a prognostic biomarker for melanoma outcome., (© 2022. The Author(s).)

Reliable serological assays are needed to understand the real impact of COVID-19. In order to compare the efficiency of different COVID-19 vaccines used in the National Vaccination Program in Tunisia, we have developed a quantitative in-house ELISA. The ELISA is based on the ectodomain of the SARS-CoV-2 Spike Baculovirus recombinant protein. We used a panel of 145 COVID-19 RT-PCR positive serum samples and 116 pre-pandemic serum samples as a negative panel. The validation was carried out by comparison to four commercial techniques (Vidas SARS-CoV-2 IgG anti-RBD Biomérieux, Elecsys Anti-Nucleocapsid of SARS-CoV-2 Roche, cPass GenScript and the quantitative Elecsys Anti-RBD of SARS-CoV-2, Roche). For the evaluation of the National Vaccination campaign, we have included 115 recipients who received one of the approved vaccines. The qualitative performances of the developed ELISA gave 96% sensitivity, 97.5% specificity and 0.968 accuracy. For the evaluation of the different brand of vaccines in recipients not previously infected with SARS-CoV-2, it seems that mRNA vaccine of Pfizer/BioNTech has shown a higher efficacy compared to inactivated virus vaccines. COVID-19 convalescent individuals have generated poor antibody responses. Nevertheless, when they are vaccinated with any brand of the COVID-19 vaccines, many of them mounted an exponential increase of the induced immune responses, qualified as a "hybrid vigor immunity". Our developed in-house ELISA seems to be very efficient in evaluating the effectiveness of anti-COVID-19 vaccination. Platforms based on mRNA vaccine are better performing than those based on inactivated virus., (© 2022. The Author(s).)

The principle of reciprocity, called Maxwell-Betti theorem, initially used in mechanics in an elastic structure, establishes a relation of equality between two distinct strains under different loads. In this paper, we extend and apply this principle to flow and solute transport equations in porous media, in order to perform the pollution sources identification in aquifers. We developed general 2D expressions of the reciprocity principle for transient transport problems. This model leads to a linear equations set, with point sources coordinates, concentrations and associated water fluxes as unknowns The proposed model is then applied to the Rocky Mountain Arsenal aquifer (Konikow in Modeling Chloride Movement in the Alluvial Aquifer at the Rocky Mountain Arsenal, Colorado. Technical Report Water-Supply Paper 2044, USGS, 1979), where polluted water is injected into a well in the domain. The used inverse technique successfully recovered the position and the pollutant concentration in addition to the associated water flux. In addition, we developed and implemented the inverse method for different knowledge levels of the degrees of the aquifer contamination, i.e. more or less data available in the field. Multiple pollution point sources and noisy data situations are also developed and tested with high efficiency. The proposed method would be easy and useful to be implemented in the modeling software now widely used by researchers and groundwater managers. It can thus be applied in real case studies, to help authorities and regulators to efficiently identify the polluters and the contamination process, i.e. its location, onset, duration and the associated mass and water fluxes., (© 2022. The Author(s).)

Temperature plays an important role in promoting the corrosion of metals. The Arrhenius plot can interpret the corrosion rate-temperature dependence, where the Arrhenius behavior gives a geometrical meaning and makes explicit a positive or negative linear dependence of charge transitivity and temperature. In addition, according to the Arrhenius interpretation, it represents the energy that the molecule in the initial state of the process must acquire before it can take part in the reaction, whether it is a physical, or a chemical process. Taking into account the deviation from the linearity, we have extended the Arrhenius-type expression by one term in 1/T 2 and we have given some physical meaning to the new related coefficients for which it is found that they depend closely on the number of acid hydrogen atoms in the polyacid for the corrosion and passivation of the Nickel based metallic glass alloy of the composition Ni 82.3 Cr 7 Fe 3 Si 4.5 B 3.2 . Moreover, we can consider that the deviation to the Arrhenius linear behavior as a super-Arrhenius behavior In addition, a mathematical analysis of the trend of experimental scatter points of the charge transfer resistance with temperature permits us to reveal an interesting homographic behavior which leads us to suggest an original empirical model with only two optimal adjustable parameters, as well as a new pseudo-power dependence of the number of hydrogen atoms in the polyacid., (© 2022. The Author(s).)

The late Middle Pleistocene, starting at around 300 ka, witnessed large-scale biological and cultural dynamics in hominin evolution across Africa including the onset of the Middle Stone Age that is closely associated with the evolution of our species-Homo sapiens. However, archaeological and geochronological data of its earliest appearance are scarce. Here we report on the late Middle Pleistocene sequence of Wadi Lazalim, in the Sahara of Southern Tunisia, which has yielded evidence for human occupations bracketed between ca. 300-130 ka. Wadi Lazalim contributes valuable information on the spread of early MSA technocomplexes across North Africa, that likely were an expression of large-scale diffusion processes., (© 2022. The Author(s).)

Using multi-electron-ion coincidence measurements combined with high level calculations, we show that double ionisation of SO 2 at 40.81 eV can be state selective. It leads to high energy products, in good yield, via a newly identified mechanism, which is likely to apply widely to multiple ionisation by almost all impact processes., (© 2021. The Author(s).)

To obtain refreshed insights into the paternal lineages of Tunisian populations, Y-chromosome diversity was assessed in two populations belonging to an Arab genealogical lineage, Kairouan and Wesletia, as well as in four Tunisian Andalusian populations, Testour, Slouguia, Qalaat-El-Andalous and El Alia. The Arabs from Kairouan revealed 73.47% of E-M81 and close affinities with Berber groups, indicating they are likely arabized Berbers, clearly differentiated from the Arabs from Wesletia, who harbored the highest frequency (71.8%) of the Middle Eastern component ever observed in North Africa. In the Tunisian Andalusians, the North African component largely prevailed, followed by the Middle Eastern contribution. Global comparative analysis highlighted the heterogeneity of Tunisian populations, among which, as a whole, dominated a set of lineages ascribed to be of autochthonous Berber origin (71.67%), beside a component of essentially Middle Eastern extraction (18.35%), and signatures of Sub-Saharan (5.2%), European (3.45%) and Asiatic (1.33%) contributions. The remarkable frequency of T-M70 in Wesletia (17.4%) prompted to refine its phylogeographic analysis, allowing to confirm its Middle Eastern origin, though signs of local evolution in Northern Africa were also detected. Evidence was clear on the ancient introduction of T lineages into the region, probably since Neolithic times associated to spread of agriculture., (© 2021. The Author(s).)

Previously, we have identified a putative novel rapidly growing Mycobacterium species, referred to as TNTM28, recovered from the sputum of an apparently immunocompetent young man with an underlying pulmonary disease. Here we provide a thorough characterization of TNTM28 genome sequence, which consists of one chromosome of 5,526,191 bp with a 67.3% G + C content, and a total of 5193 predicted coding sequences. Phylogenomic analyses revealed a deep-rooting relationship to the Mycobacterium fortuitum complex, thus suggesting a new taxonomic entity. TNTM28 was predicted to be a human pathogen with a probability of 0.804, reflecting the identification of several virulence factors, including export systems (Sec, Tat, and ESX), a nearly complete set of Mce proteins, toxin-antitoxins systems, and an extended range of other genes involved in intramacrophage replication and persistence (hspX, ahpC, sodA, sodC, katG, mgtC, ClpR, virS, etc.), some of which had likely been acquired through horizontal gene transfer. Such an arsenal of potential virulence factors, along with an almost intact ESX-1 locus, might have significantly contributed to TNTM28 pathogenicity, as witnessed by its ability to replicate efficiently in macrophages. Overall, the identification of this new species as a potential human pathogen will help to broaden our understanding of mycobacterial pathogenesis.

Estimating unsaturated soil hydraulic properties to predict water dynamics through a vertical soil profile under the effects of irrigation, drainage and evapotranspiration is imperative for managing soils in the arid regions. The aim of this work was to determine the soil water retention curve and the hydraulic permeability function of a bare soil profile in a Tunisian oasis threatened by salinization. The developed model combines a numerical inversion of the unsaturated flow equation with the BOBYQA optimisation algorithm. The direct model solved the Richards equation using a cell-centred finite difference model. Hydraulic properties were described by van Genuchten-Mualem models. Input data for the inverse problem are the infiltration flow, soil water contents and pressure heads measured during ponded infiltration and internal drainage tests. Numerical simulations of these two tests were performed considering a homogeneous single-layer soil profile but a better fitting between measured and simulated water contents was obtained when the soil profile was divided into five sub-layers. The hysteresis phenomenon was highlighted from the soil water retention and the relative permeability curves corresponding to the ponded infiltration and internal drainage tests.

The effect of gamma-irradiation and ionizing radiation (high-energy electrons beam) on the physicochemical properties of metoprolol tartrate at the solid phase and aqueous solution, has been investigated in the present study to model some properties affected by absorbed doses and to reveal some interesting mutual causal correlation. The proposed some interesting models can be adapted to other experimental conditions, and the newly obtained values of the adjustable parameters could be an excellent criterion of the state quality of the metoprolol tartrate or for other additional interpretations. The peculiar behaviour of variation of physicochemical properties against dose leads us to confirm the suggested optimized doses mentioned in previous work, for sterilization and safe medical uses.

Systemic sclerosis (SSc) is an autoimmune disorder characterized by vascular damage, excessive fibrosis and abnormal T cells immune-regulation. CD146 is an adhesion molecule essentially expressed in the vascular system, but also on TH17 lymphocytes. In view of the recently described role of CD146 in SSc, we hypothesized an involvement of CD146 positive TH17 cells in this disease. Compared to healthy controls, we showed that both soluble form of CD146 (sCD146), and IL17A levels were increased in patients with SSc with a positive correlation between both factors. A significant increase in TH17 cells attested by an increase of RORγT, IL17A mRNA and CD4+ IL17A+ cell was observed in patients with SSc. Interestingly, the percentage of TH17 cells expressing CD146 was higher in patients with SSc and inversely correlated with pulmonary fibrosis. In vitro experiments showed an augmentation of the percentage of TH17 cells expressing CD146 after cell treatment with sCD146, suggesting that, in patients the increase of this sub-population could be the consequence of the sCD146 increase in serum. In conclusion, TH17 cells expressing CD146 could represent a new component of the adaptive immune response, opening the way for the generation of new tools for the management of SSc.

Coxsackieviruses B (CV-B) belong to the EV-B species. CV-B and particularly CV-B4 are thought to be involved in the development of chronic diseases like type 1 diabetes (T1D). The mechanisms of the enteroviral pathogenesis of T1D are not well known, yet. The in vitro studies are rich with information but in vivo infection models are needed to investigate the impact of viruses onto organs. Our objective was to study the impact of CV-B4E2 combined with a single sub-diabetogenic dose of streptozotocin (STZ) on the pancreas of mice. The infection with CV-B4E2 of CD1 outbred mice treated with a sub-diabetogenic dose of STZ induced hyperglycemia and hypoinsulinemia. Along with the chemokine IP-10, viral RNA and infectious particles were detected in the pancreas. The pancreas of these animals was also marked with insulitis and other histological alterations. The model combining STZ and CV-B4E2 opens the door to new perspectives to better understand the interactions between virus and host, and the role of environmental factors capable, like STZ, to predispose the host to the diabetogenic effects of enteroviruses.

Resistance to 5-Fluorouracil chemotherapy is a major cause of therapeutic failure in colon cancer cure. Development of combined therapies constitutes an effective strategy to inhibit cancer cells and prevent the emergence of drug resistance. For this purpose, we investigated the anti-tumoral effect of thirteen phenolic compounds, from the Tunisian quince Cydonia oblonga Miller, alone or combined to 5-FU, on the human 5-FU-resistant LS174-R colon cancer cells in comparison to parental cells. Our results showed that only Kaempferol was able to chemo-sensitize 5-FU-resistant LS174-R cells. This phenolic compound combined with 5-FU exerted synergistic inhibitory effect on cell viability. This combination enhanced the apoptosis and induced cell cycle arrest of both chemo-resistant and sensitive cells through impacting the expression levels of different cellular effectors. Kaempferol also blocked the production of reactive oxygen species (ROS) and modulated the expression of JAK/STAT3, MAPK, PI3K/AKT and NF-κB. In silico docking analysis suggested that the potent anti-tumoral effect of Kaempferol, compared to its two analogs (Kaempferol 3-O-glucoside and Kampferol 3-O-rutinoside), can be explained by the absence of glucosyl groups. Overall, our data propose Kaempferol as a potential chemotherapeutic agent to be used alone or in combination with 5-FU to overcome colon cancer drug resistance.

To our knowledge, the phylodistribution of M. hominis clinical strains associated with various pathological conditions of the urogenital tract has not been explored hitherto. Here we analyzed the genetic diversity and phylogenetic relationships among 59 M. hominis Tunisian clinical isolates, categorized as gynecological infections- or infertility-associated pathotypes. For this purpose, we developed an expanded multilocus sequence typing (eMLST) scheme, combining the previously reported multilocus sequence typing (MLST) loci (gyrB, tuf, ftsY, uvrA, gap) with a new selected set of putative virulence genes (p120', vaa, lmp1, lmp3, p60), referred herein to as multi-virulence-locus sequence typing (MVLST) loci. In doing so, M. hominis population was segregated into two distinct genetic lineages, which were differentially associated with each pathotype. Such a clear dichotomy was supported by several phylogenetic and population genetic analysis tools. Recombination was found to take place, but not sufficient enough to break down the overall clonal population structure of M. hominis, most likely as a result of purifying selection, which accommodated the most fit clones. In sum, and owing to the eMLST scheme described herein, we provide insightful data on the phylogenetics of M. hominis, arguing for the existence of genetically differentiable urogenital pathotypes.

Actinorhizal plants are ecologically and economically important. Symbiosis with nitrogen-fixing bacteria allows these woody dicotyledonous plants to colonise soils under nitrogen deficiency, water-stress or other extreme conditions. However, proteins involved in xerotolerance of symbiotic microorganisms have yet to be identified. Here we characterise the polyethylene glycol (PEG)-responding desiccome from the most geographically widespread Gram-positive nitrogen-fixing plant symbiont, Frankia alni, by next-generation proteomics, taking advantage of a Q-Exactive HF tandem mass spectrometer equipped with an ultra-high-field Orbitrap analyser. A total of 2,052 proteins were detected and quantified. Under osmotic stress, PEG-grown F. alni cells increased the abundance of envelope-associated proteins like ABC transporters, mechano-sensitive ion channels and Clustered Regularly Interspaced Short Palindromic Repeats CRISPR-associated (cas) components. Conjointly, dispensable pathways, like nitrogen fixation, aerobic respiration and homologous recombination, were markedly down-regulated. Molecular modelling and docking simulations suggested that the PEG is acting on Frankia partly by filling the inner part of an up-regulated osmotic-stress large conductance mechanosensitive channel.

We have investigated the electronic response of single crystals of indium selenide by means of angle-resolved photoemission spectroscopy, electron energy loss spectroscopy and density functional theory. The loss spectrum of indium selenide shows the direct free exciton at ~1.3 eV and several other peaks, which do not exhibit dispersion with the momentum. The joint analysis of the experimental band structure and the density of states indicates that spectral features in the loss function are strictly related to single-particle transitions. These excitations cannot be considered as fully coherent plasmons and they are damped even in the optical limit, i.e. for small momenta. The comparison of the calculated symmetry-projected density of states with electron energy loss spectra enables the assignment of the spectral features to transitions between specific electronic states. Furthermore, the effects of ambient gases on the band structure and on the loss function have been probed.

Bicomponent pore-forming leukocidins are a family of potent toxins secreted by Staphylococcus aureus, which target white blood cells preferentially and consist of an S- and an F-component. The S-component recognizes a receptor on the host cell, enabling high-affinity binding to the cell surface, after which the toxins form a pore that penetrates the cell lipid bilayer. Until now, six different leukocidins have been described, some of which are host and cell specific. Here, we identify and characterise a novel S. aureus leukocidin; LukPQ. LukPQ is encoded on a 45 kb prophage (ΦSaeq1) found in six different clonal lineages, almost exclusively in strains cultured from equids. We show that LukPQ is a potent and specific killer of equine neutrophils and identify equine-CXCRA and CXCR2 as its target receptors. Although the S-component (LukP) is highly similar to the S-component of LukED, the species specificity of LukPQ and LukED differs. By forming non-canonical toxin pairs, we identify that the F-component contributes to the observed host tropism of LukPQ, thereby challenging the current paradigm that leukocidin specificity is driven solely by the S-component.

Bacteriophages display remarkable genetic diversity and host specificity. In this study, we explore phages infecting bacterial strains of the Enterobacteriaceae family because of their ability to infect related but distinct hosts. We isolated and characterized two novel virulent phages, SH6 and SH7, using a strain of Shigella flexneri as host bacterium. Morphological and genomic analyses revealed that phage SH6 belongs to the T1virus genus of the Siphoviridae family. Conversely, phage SH7 was classified in the T4virus genus of the Myoviridae family. Phage SH6 had a short latent period of 16 min and a burst size of 103 ± 16 PFU/infected cell while the phage SH7 latent period was 23 min with a much lower burst size of 26 ± 5 PFU/infected cell. Moreover, phage SH6 was sensitive to acidic conditions (pH < 5) while phage SH7 was stable from pH 3 to 11 for 1 hour. Of the 35 bacterial strains tested, SH6 infected its S. flexneri host strain and 8 strains of E. coli. Phage SH7 lysed additionally strains of E. coli O157:H7, Salmonella Paratyphi, and Shigella dysenteriae. The broader host ranges of these two phages as well as their microbiological properties suggest that they may be useful for controlling bacterial populations.

The repeated failures reported in cultivating some microbial lineages are a major challenge in microbial ecology and probably linked, in the case of Frankia microsymbionts to atypical patterns of auxotrophy. Comparative genomics of the so far uncultured cluster-2 Candidatus Frankia datiscae Dg1, with cultivated Frankiae has revealed genome reduction, but no obvious physiological impairments. A direct physiological assay on nodule tissues from Coriaria myrtifolia infected with a closely-related strain permitted the identification of a requirement for alkaline conditions. A high pH growth medium permitted the recovery of a slow-growing actinobacterium. The strain obtained, called BMG5.1, has short hyphae, produced diazovesicles in nitrogen-free media, and fulfilled Koch's postulates by inducing effective nodules on axenically grown Coriaria spp. and Datisca glomerata. Analysis of the draft genome confirmed its close proximity to the Candidatus Frankia datiscae Dg1 genome with the absence of 38 genes (trehalose synthase, fumarylacetoacetase, etc) in BMG5.1 and the presence of 77 other genes (CRISPR, lanthionine synthase, glutathione synthetase, catalase, Na+/H+ antiporter, etc) not found in Dg1. A multi-gene phylogeny placed the two cluster-2 strains together at the root of the Frankia radiation.