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Start Over Author "Shuang Zeng" Journal scientific reports
4 results on '"Shuang Zeng"'

1. A comprehensive whole genome database of ethnic minority populations

Author

Yan He, Changgui Lei, Chanjuan Wan, Shuang Zeng, Ting Zhang, Fei Luo, Ruichao Li, Xiaokun Li, Anshu Zhao, Defu Xiao, Yunyan Luo, Keren Shan, Xiaolan Qi, and Xin Jin

Subjects
GMGD database, Human whole genome sequencing, Guizhou Province in southwest China, Ethnic minority populations, Medicine, Science
Abstract

Abstract China, is characterized by its remarkable ethnical diversity, which necessitates whole genome variation data from multiple populations as crucial tools for advancing population genetics and precision medical research. However, there has been a scarcity of research concentrating on the whole genome of ethnic minority groups. To fill this gap, we developed the Guizhou Multi-ethnic Genome Database (GMGD). It comprises whole genome sequencing data from 476 healthy unrelated individuals spanning 11 ethnic minorities groups in Guizhou Province, Southwest China, including Bouyei, Dong, Miao, Yi, Bai, Gelo, Zhuang, Tujia, Yao, Hui, and Sui. The GMGD database comprises more than 16.33 million variants in GRCh38 and 16.20 million variants in GRCh37. Among these, approximately 11.9% (1,956,322) of the variants in GRCh38 and 18.5% (3,009,431) of the variants in GRCh37 are entirely new and do not exist in the dbSNP database. These novel variants shed light on the genetic diversity landscape across these populations, providing valuable insights with an average coverage of 5.5 ×. This makes GMGD the largest genome-wide database encompassing the most diverse ethnic groups to date. The GMGD interactive interface facilitates researchers with multi-dimensional mutation search methods and displays population frequency differences among global populations. Furthermore, GMGD is equipped with a genotype-imputation function, enabling enhanced capabilities for low-depth genomic research or targeted region capture studies. GMGD offers unique insights into the genomic variation landscape of different ethnic groups, which are freely accessible at https://db.cngb.org/pop/gmgd/ .

Published
2024
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3. Monoclonal and oligoclonal TCR AV and BV gene usage in CD4+ T cells from pigs immunised with C-strain CSFV vaccine

Author

Wenyu Cheng, Guohua Chen, Shoujie Li, Hong Yin, Chun-Yan Wang, Qiwang Jin, Huaijie Jia, Fang Yongxiang, Jing Zhizhong, Xiaobing He, Shuang Zeng, Wenjuan Yao, and Yuan Feng

Subjects
0301 basic medicine, Cellular immunity, Multidisciplinary, biology, Viral Vaccine, T cell, lcsh:R, T-cell receptor, lcsh:Medicine, hemic and immune systems, chemical and pharmacologic phenomena, Complementarity determining region, biology.organism_classification, Virology, Epitope, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, medicine.anatomical_structure, Antigen, Classical swine fever, medicine, lcsh:Q, lcsh:Science, 030215 immunology
Abstract

The classical swine fever virus C-strain vaccine (C-strain vaccine) plays a vital role in preventing and controlling the spread of classical swine fever (CSF). However, the protective mechanisms of C-strain vaccine and cellular immunity conferred by T cell receptors (TCRs) are less well defined. We aimed to analyse the association between the complementarity determining region 3 (CDR3) spectratype of αβTCR in CD4+ T cells and C-strain vaccine; and to find conserved CDR3 amino acid motifs in specific TCR α- and β-chains. We found that the CDR3 spectratype showed dynamic changes correlating with C-strain vaccine immunisation and that TCR AV5S/8–3S/8–4S/14/38 and BV4S/6S/7S/15S/30 gene families showed clonal expansion in immunised pigs. The sequences of CDR3 from these clonally expanded T cells indicated a high frequency of the ‘KLX’ motif in the TCR α chain and the ‘GGX’ motif in β chain, and Jα39, Jα43, Jβ2.5 and Jβ2.3 genes were also found in high frequency. To the best of our knowledge, this is the first report describing the dynamic changes of αβTCRs and conserved CDR3 amino acid motifs in CD4+ T cells from C-strain vaccine-immunised pigs, which will provide a basis for the development of high-efficiency epitope vaccines.

Published
2018

4. Monoclonal and oligoclonal TCR AV and BV gene usage in CD4

Author

Chunyan, Wang, Shoujie, Li, Huaijie, Jia, Guohua, Chen, Yongxiang, Fang, Shuang, Zeng, Xiaobing, He, Wenjuan, Yao, Qiwang, Jin, Wenyu, Cheng, Yuan, Feng, Hong, Yin, and Zhizhong, Jing

Subjects
CD4-Positive T-Lymphocytes, Classical Swine Fever Virus, Swine, Multigene Family, Receptors, Antigen, T-Cell, alpha-beta, Animals, hemic and immune systems, chemical and pharmacologic phenomena, Viral Vaccines, Sequence Analysis, DNA, Complementarity Determining Regions, Article
Abstract

The classical swine fever virus C-strain vaccine (C-strain vaccine) plays a vital role in preventing and controlling the spread of classical swine fever (CSF). However, the protective mechanisms of C-strain vaccine and cellular immunity conferred by T cell receptors (TCRs) are less well defined. We aimed to analyse the association between the complementarity determining region 3 (CDR3) spectratype of αβTCR in CD4+ T cells and C-strain vaccine; and to find conserved CDR3 amino acid motifs in specific TCR α- and β-chains. We found that the CDR3 spectratype showed dynamic changes correlating with C-strain vaccine immunisation and that TCR AV5S/8–3S/8–4S/14/38 and BV4S/6S/7S/15S/30 gene families showed clonal expansion in immunised pigs. The sequences of CDR3 from these clonally expanded T cells indicated a high frequency of the ‘KLX’ motif in the TCR α chain and the ‘GGX’ motif in β chain, and Jα39, Jα43, Jβ2.5 and Jβ2.3 genes were also found in high frequency. To the best of our knowledge, this is the first report describing the dynamic changes of αβTCRs and conserved CDR3 amino acid motifs in CD4+ T cells from C-strain vaccine-immunised pigs, which will provide a basis for the development of high-efficiency epitope vaccines.

Published
2017

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