5 results on '"Sebastian Siebelmann"'
Search Results
2. A deep learning approach for successful big-bubble formation prediction in deep anterior lamellar keratoplasty
- Author
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Takahiko Hayashi, Hiroki Masumoto, Hitoshi Tabuchi, Naofumi Ishitobi, Mao Tanabe, Michael Grün, Björn Bachmann, Claus Cursiefen, and Sebastian Siebelmann
- Subjects
Medicine ,Science - Abstract
Abstract The efficacy of deep learning in predicting successful big-bubble (SBB) formation during deep anterior lamellar keratoplasty (DALK) was evaluated. Medical records of patients undergoing DALK at the University of Cologne, Germany between March 2013 and July 2019 were retrospectively analyzed. Patients were divided into two groups: (1) SBB or (2) failed big-bubble (FBB). Preoperative images of anterior segment optical coherence tomography and corneal biometric values (corneal thickness, corneal curvature, and densitometry) were evaluated. A deep neural network model, Visual Geometry Group-16, was selected to test the validation data, evaluate the model, create a heat map image, and calculate the area under the curve (AUC). This pilot study included 46 patients overall (11 women, 35 men). SBBs were more common in keratoconus eyes (KC eyes) than in corneal opacifications of other etiologies (non KC eyes) (p = 0.006). The AUC was 0.746 (95% confidence interval [CI] 0.603–0.889). The determination success rate was 78.3% (18/23 eyes) (95% CI 56.3–92.5%) for SBB and 69.6% (16/23 eyes) (95% CI 47.1–86.8%) for FBB. This automated system demonstrates the potential of SBB prediction in DALK. Although KC eyes had a higher SBB rate, no other specific findings were found in the corneal biometric data.
- Published
- 2021
- Full Text
- View/download PDF
3. Transient Ingrowth of Lymphatic Vessels into the Physiologically Avascular Cornea Regulates Corneal Edema and Transparency
- Author
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Deniz Hos, Anne Bukowiecki, Jens Horstmann, Felix Bock, Franziska Bucher, Ludwig M. Heindl, Sebastian Siebelmann, Philipp Steven, Reza Dana, Sabine A. Eming, and Claus Cursiefen
- Subjects
Medicine ,Science - Abstract
Abstract Lymphangiogenesis is essential for fluid homeostasis in vascularized tissues. In the normally avascular cornea, however, pathological lymphangiogenesis mediates diseases like corneal transplant rejection, dry eye disease, and allergy. So far, a physiological role for lymphangiogenesis in a primarily avascular site such as the cornea has not been described. Using a mouse model of perforating corneal injury that causes acute and severe fluid accumulation in the cornea, we show that lymphatics transiently and selectively invade the cornea and regulate the resolution of corneal edema. Pharmacological blockade of lymphangiogenesis via VEGFR-3 inhibition results in increased corneal thickness due to delayed drainage of corneal edema and a trend towards prolonged corneal opacification. Notably, lymphatics are also detectable in the cornea of a patient with acute edema due to spontaneous Descemet´s (basement) membrane rupture in keratoconus, mimicking this animal model and highlighting the clinical relevance of lymphangiogenesis in corneal fluid homeostasis. Together, our findings provide evidence that lymphangiogenesis plays an unexpectedly beneficial role in the regulation of corneal edema and transparency. This might open new treatment options in blinding diseases associated with corneal edema and transparency loss. Furthermore, we demonstrate for the first time that physiological lymphangiogenesis also occurs in primarily avascular sites.
- Published
- 2017
- Full Text
- View/download PDF
4. Risk factors for endothelial cell loss after Descemet membrane endothelial keratoplasty (DMEK)
- Author
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Viet Nhat Hung Le, Björn Bachmann, Silvia Schrittenlocher, Jeremy Franklin, Mario Matthaei, Sebastian Siebelmann, Claus Cursiefen, and Takahiko Hayashi
- Subjects
Graft Rejection ,Male ,medicine.medical_specialty ,Visual acuity ,genetic structures ,Descemet membrane ,Corneal diseases ,Visual Acuity ,lcsh:Medicine ,Diseases ,Organ culture ,Article ,03 medical and health sciences ,Organ Culture Techniques ,Postoperative Complications ,0302 clinical medicine ,Risk Factors ,Ophthalmology ,Humans ,Medicine ,Sulphur Hexafluoride ,Prospective Studies ,lcsh:Science ,Eye diseases ,Aged ,Retrospective Studies ,Multidisciplinary ,business.industry ,lcsh:R ,Endothelium, Corneal ,Fuchs' Endothelial Dystrophy ,Graft Survival ,Significant difference ,Tissue Donors ,Endothelial cell density ,Endothelial stem cell ,030221 ophthalmology & optometry ,lcsh:Q ,Female ,Tamponade ,medicine.symptom ,business ,Descemet Stripping Endothelial Keratoplasty ,030217 neurology & neurosurgery - Abstract
This study aimed to identify the risk factors for endothelial cell density (ECD) loss after Descemet membrane endothelial keratoplasty (DMEK) and analyse whether donor tissues from cold versus organ culture differ in terms of ECD loss after DMEK. Consecutive DMEK cases from a prospective database for Fuchs’ endothelial corneal dystrophy were retrospectively analysed between 2011 and 2016 at the University of Cologne, and the possible risk factors for ECD loss, including patient-related factors, type of tamponade (air or 20% sulphur hexafluoride gas), type of surgery (triple DMEK or DMEK alone), re-bubbling, immune rejection, and donor-related factors were determined. Eight hundred and forty-one eyes were selected. There was no significant difference in the best-corrected visual acuity (logarithm of the minimal angle of resolution) and corneal thickness (P = 0.540 and P = 0.667) between groups. Immune reactions were more common in cold cultures (P = 0.019), but ECD loss (1 year after DMEK) was greater in organ cultures (38.3 ± 0.8%) than in cold cultures (34.7 ± 1.4%) (P = 0.022). Only re-bubbling was significantly associated with ECD loss (P
- Published
- 2020
5. Transient Ingrowth of Lymphatic Vessels into the Physiologically Avascular Cornea Regulates Corneal Edema and Transparency
- Author
-
Sebastian Siebelmann, Ludwig M. Heindl, Felix Bock, Franziska Bucher, Sabine A. Eming, Reza Dana, Claus Cursiefen, Anne Bukowiecki, Jens Horstmann, Philipp Steven, and Deniz Hos
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,Keratoconus ,genetic structures ,Science ,Gene Expression ,Article ,Cornea ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Corneal edema ,Corneal Injury ,medicine ,Animals ,Humans ,Corneal Neovascularization ,Lymphangiogenesis ,Lymphatic Vessels ,Multidisciplinary ,business.industry ,Corneal Edema ,medicine.disease ,Immunohistochemistry ,eye diseases ,Disease Models, Animal ,030104 developmental biology ,medicine.anatomical_structure ,Lymphatic system ,Corneal neovascularization ,030221 ophthalmology & optometry ,Medicine ,Female ,sense organs ,business ,Biomarkers ,Corneal Injuries - Abstract
Lymphangiogenesis is essential for fluid homeostasis in vascularized tissues. In the normally avascular cornea, however, pathological lymphangiogenesis mediates diseases like corneal transplant rejection, dry eye disease, and allergy. So far, a physiological role for lymphangiogenesis in a primarily avascular site such as the cornea has not been described. Using a mouse model of perforating corneal injury that causes acute and severe fluid accumulation in the cornea, we show that lymphatics transiently and selectively invade the cornea and regulate the resolution of corneal edema. Pharmacological blockade of lymphangiogenesis via VEGFR-3 inhibition results in increased corneal thickness due to delayed drainage of corneal edema and a trend towards prolonged corneal opacification. Notably, lymphatics are also detectable in the cornea of a patient with acute edema due to spontaneous Descemet´s (basement) membrane rupture in keratoconus, mimicking this animal model and highlighting the clinical relevance of lymphangiogenesis in corneal fluid homeostasis. Together, our findings provide evidence that lymphangiogenesis plays an unexpectedly beneficial role in the regulation of corneal edema and transparency. This might open new treatment options in blinding diseases associated with corneal edema and transparency loss. Furthermore, we demonstrate for the first time that physiological lymphangiogenesis also occurs in primarily avascular sites.
- Published
- 2017
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