1. Molecular recognition of wood polyphenols by phase II detoxification enzymes of the white rot Trametes versicolor
- Author
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Schwartz, Mathieu, Perrot, Thomas, Aubert, Emmanuel, Dumarçay, Stéphane, Favier, Frédérique, Gérardin, Philippe, Morel-Rouhier, Mélanie, Mulliert, Guillermo, Saiag, Fanny, Didierjean, Claude, Gelhaye, Eric, Cristallographie, Résonance Magnétique et Modélisations (CRM2), Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Interactions Arbres-Microorganismes (IAM), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Laboratoire d'Etude et de Recherche sur le Matériau Bois (LERMAB), Université de Lorraine (UL), ANR (ANR-11-LAS-0002-01), Centre National de la Recherche Scientifique, University of Lorraine, Region Grand Est, and Université de Lorraine (UL)-Institut National de la Recherche Agronomique (INRA) more...
- Subjects
PRUNUS-AVIUM ,STRUCTURAL EVIDENCE ,GLUTATHIONE TRANSFERASES ,cerisier ,[SDV]Life Sciences [q-bio] ,lcsh:Medicine ,reconnaissance moléculaire ,Crystallography, X-Ray ,Article ,cristallographie macromoléculaire ,Fungal Proteins ,polyphénol ,Benzophenones ,trametes versicolor ,champignon du bois ,enzyme de détoxification ,Amino Acid Sequence ,ADAPTATION ,lcsh:Science ,Glutathione Transferase ,Flavonoids ,Trametes ,cherry tree ,Binding Sites ,IDENTIFICATION ,Protein Stability ,CRYSTALLOGRAPHY ,lcsh:R ,Temperature ,FUNGI ,Polyphenols ,food and beverages ,EXTRACTIVES ,Wood ,Metabolic Detoxication, Phase II ,Protein Structure, Tertiary ,Isoenzymes ,Kinetics ,INSECTICIDE RESISTANCE ,dégradation du bois ,lcsh:Q ,molecular recognition ,Prunus ,Sequence Alignment - Abstract
Wood decay fungi have complex detoxification systems that enable them to cope with secondary metabolites produced by plants. Although the number of genes encoding for glutathione transferases is especially expanded in lignolytic fungi, little is known about their target molecules. In this study, by combining biochemical, enzymatic and structural approaches, interactions between polyphenols and six glutathione transferases from the white-rot fungus Trametes versicolor have been demonstrated. Two isoforms, named TvGSTO3S and TvGSTO6S have been deeply studied at the structural level. Each isoform shows two distinct ligand-binding sites, a narrow L-site at the dimer interface and a peculiar deep hydrophobic H-site. In TvGSTO3S, the latter appears optimized for aromatic ligand binding such as hydroxybenzophenones. Affinity crystallography revealed that this H-site retains the flavonoid dihydrowogonin from a partially purified wild-cherry extract. Besides, TvGSTO6S binds two molecules of the flavonoid naringenin in the L-site. These data suggest that TvGSTO isoforms could interact with plant polyphenols released during wood degradation. more...
- Published
- 2018
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