43 results on '"Paquet A"'
Search Results
2. Modeling and optimization of bakery production scheduling to minimize makespan and oven idle time
- Author
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Babor, Majharulislam, Paquet-Durand, Olivier, Kohlus, Reinhard, and Hitzmann, Bernd
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- 2023
- Full Text
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3. Cost consequence analysis of waiting for lumbar disc herniation surgery
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Charlotte Dandurand, Mohammad Sadegh Mashayekhi, Greg McIntosh, Supriya Singh, Jerome Paquet, Hasaan Chaudhry, Edward Abraham, Christopher S. Bailey, Michael H. Weber, Michael G. Johnson, Andrew Nataraj, Najmedden Attabib, Adrienne Kelly, Hamilton Hall, Y. Raja Rampersaud, Neil Manson, Philippe Phan, Ken Thomas, Charles Fisher, Raphaele Charest-Morin, Alex Soroceanu, Bernard LaRue, and Nicolas Dea
- Subjects
Medicine ,Science - Abstract
Abstract The economic repercussions of waiting for lumbar disc surgery have not been well studied. The primary goal of this study was to perform a cost-consequence analysis of patients receiving early vs late surgery for symptomatic disc herniation from a societal perspective. Secondarily, we compared patient factors and patient-reported outcomes. This is a retrospective analysis of prospectively collected data from the CSORN registry. A cost-consequence analysis was performed where direct and indirect costs were compared, and different outcomes were listed separately. Comparisons were made on an observational cohort of patients receiving surgery less than 60 days after consent (short wait) or 60 days or more after consent (long wait). This study included 493 patients with surgery between January 2015 and October 2021 with 272 patients (55.2%) in the short wait group and 221 patients (44.8%) classified as long wait. There was no difference in proportions of patients who returned to work at 3 and 12-months. Time from surgery to return to work was similar between both groups (34.0 vs 34.9 days, p = 0.804). Time from consent to return to work was longer in the longer wait group corresponding to an additional $11,753.10 mean indirect cost per patient. The short wait group showed increased healthcare usage at 3 months with more emergency department visits (52.6% vs 25.0%, p
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- 2023
- Full Text
- View/download PDF
4. Modeling and optimization of bakery production scheduling to minimize makespan and oven idle time
- Author
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Majharulislam Babor, Olivier Paquet-Durand, Reinhard Kohlus, and Bernd Hitzmann
- Subjects
Medicine ,Science - Abstract
Abstract Makespan dominates the manufacturing expenses in bakery production. The high energy consumption of ovens also has a substantial impact, which bakers may overlook. Bakers leave ovens running until the final product is baked, allowing them to consume energy even when not in use. It results in energy waste, increased manufacturing costs, and CO2 emissions. This paper investigates three manufacturing lines from small and medium-sized bakeries to find optimum makespan and ovens’ idle time (OIDT). A hybrid no-wait flow shop scheduling model considering the constraints that are most common in bakeries is proposed. To find optimal solutions, non-dominated sorting genetic algorithm (NSGA-II), strength Pareto evolutionary algorithm (SPEA2), generalized differential evolution (GDE3), improved multi-objective particle swarm optimization (OMOPSO), and speed-constrained multi-objective particle swarm optimization (SMPSO) were used. The experimental results show that the shortest makespan does not always imply the lowest OIDT. Even the optimized solutions have up to 231 min of excess OIDT, while the makespan is the shortest. Pareto solutions provide promising trade-offs between makespan and OIDT, with the best-case scenario reducing OIDT by 1348 min while increasing makespan only by 61 min from the minimum possible makespan. NSGA-II outperforms all other algorithms in obtaining a high number of good-quality solutions and a small number of poor-quality solutions, followed by SPEA2 and GDE3. In contrast, OMOPSO and SMPSO deliver the worst solutions, which become pronounced as the problem complexity grows.
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- 2023
- Full Text
- View/download PDF
5. Postoperative recovery patterns following discectomy surgery in patients with lumbar radiculopathy
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Shuaijin Wang, Jeffrey J. Hebert, Edward Abraham, Amanda Vandewint, Erin Bigney, Eden Richardson, Dana El-Mughayyar, Najmedden Attabib, Niels Wedderkopp, Stephen Kingwell, Alex Soroceanu, M. H. Weber, Hamilton Hall, Joel Finkelstein, Christopher S. Bailey, Kenneth Thomas, Andrew Nataraj, Jerome Paquet, Michael G. Johnson, Charles Fisher, Y. Raja Rampersaud, Nicolas Dea, Chris Small, and Neil Manson
- Subjects
Medicine ,Science - Abstract
Abstract This retrospective study of prospectively collected data aimed to identify unique pain and disability trajectories in patients following lumbar discectomy surgery. Patients of this study population presented chiefly with lumbar radiculopathy and underwent discectomy surgery from thirteen sites enrolled in the CSORN registry. Outcome variables of interest included numeric rating scales for leg/back pain and modified Oswestry disability index scores at baseline, 3, 12, and 24 months post-operatively. Latent class growth analysis was used to identify distinct courses in each outcome. Data from 524 patients revealed three unique trajectories for leg pain (excellent = 18.4%, good = 55.4%, poor = 26.3%), disability (excellent = 59.7%, fair = 35.6%, poor = 4.7%) and back pain (excellent = 13.0%, good = 56.4%, poor = 30.6%). Construct validity was supported by statistically significant differences in the proportions of patients attaining the criteria for minimal important change (MIC; 30%) or clinical success in disability (50% or Oswestry score ≤ 22) (p
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- 2022
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6. Postoperative recovery patterns following discectomy surgery in patients with lumbar radiculopathy
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Wang, Shuaijin, Hebert, Jeffrey J., Abraham, Edward, Vandewint, Amanda, Bigney, Erin, Richardson, Eden, El-Mughayyar, Dana, Attabib, Najmedden, Wedderkopp, Niels, Kingwell, Stephen, Soroceanu, Alex, Weber, M. H., Hall, Hamilton, Finkelstein, Joel, Bailey, Christopher S., Thomas, Kenneth, Nataraj, Andrew, Paquet, Jerome, Johnson, Michael G., Fisher, Charles, Rampersaud, Y. Raja, Dea, Nicolas, Small, Chris, and Manson, Neil
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- 2022
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7. Spectroscopic analysis of chia seeds
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Mburu, Monica, Paquet-Durand, Olivier, Hitzmann, Bernd, and Zettel, Viktoria
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- 2021
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8. Whimbrel populations differ in trans-atlantic pathways and cyclone encounters
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Watts, Bryan D., Smith, Fletcher M., Hines, Chance, Duval, Laura, Hamilton, Diana J., Keyes, Tim, Paquet, Julie, Pirie-Dominix, Lisa, Rausch, Jennie, Truitt, Barry, Winn, Brad, and Woodard, Paul
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- 2021
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9. Masking terminal neo-epitopes of linear peptides through glycosylation favours immune responses towards core epitopes producing parental protein bound antibodies
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Pon, Robert, Marcil, Anne, Chen, Wangxue, Gadoury, Christine, Williams, Dean, Chan, Kenneth, Zhou, Hongyan, Ponce, Amalia, Paquet, Eric, Gurnani, Komal, Chattopadhyay, Anindita, and Zou, Wei
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- 2020
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10. Opioid drug use in emergency and adverse outcomes among patients with chronic obstructive pulmonary disease: a multicenter observational study
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Viglino, Damien, Daoust, Raoul, Bailly, Sebastien, Faivre-Pierret, Caroline, Charif, Isma, Roustit, Matthieu, Paquet, Jean, Debaty, Guillaume, Pépin, Jean-Louis, Maignan, Maxime, and Chauny, Jean-Marc
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- 2020
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11. New protein-DNA complexes in archaea: a small monomeric protein induces a sharp V-turn DNA structure
- Author
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Loth, Karine, Largillière, Justine, Coste, Franck, Culard, Françoise, Landon, Céline, Castaing, Bertrand, Delmas, Agnès F., and Paquet, Françoise
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- 2019
- Full Text
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12. Long-distance migratory shorebirds travel faster towards their breeding grounds, but fly faster post-breeding
- Author
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Duijns, Sjoerd, Anderson, Alexandra M., Aubry, Yves, Dey, Amanda, Flemming, Scott A., Francis, Charles M., Friis, Christian, Gratto-Trevor, Cheri, Hamilton, Diana J., Holberton, Rebecca, Koch, Stephanie, McKellar, Ann E., Mizrahi, David, Morrissey, Christy A., Neima, Sarah G., Newstead, David, Niles, Larry, Nol, Erica, Paquet, Julie, Rausch, Jennie, Tudor, Lindsay, Turcotte, Yves, and Smith, Paul A.
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- 2019
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13. A retinal model of cerebral malaria
- Author
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Paquet-Durand, François, Beck, Susanne C., Das, Soumyaparna, Huber, Gesine, Le Chang, Schubert, Timm, Tanimoto, Naoyuki, Garcia-Garrido, Marina, Mühlfriedel, Regine, Bolz, Sylvia, Hoffmann, Wolfgang, Schraermeyer, Ulrich, Mordmüller, Benjamin, and Seeliger, Mathias W.
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- 2019
- Full Text
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14. The paracrine effects of human induced pluripotent stem cells promote bone-like structures via the upregulation of BMP expression in a mouse ectopic model
- Author
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Oudina, Karim, Paquet, Joseph, Moya, Adrien, Massourides, Emmanuelle, Bensidhoum, Morad, Larochette, Nathanaël, Deschepper, Mickael, Pinset, Christian, and Petite, Hervé
- Published
- 2018
- Full Text
- View/download PDF
15. Modeling and optimization of bakery production scheduling to minimize makespan and oven idle time
- Author
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Majharulislam Babor, Olivier Paquet-Durand, Reinhard Kohlus, and Bernd Hitzmann
- Subjects
Multidisciplinary ,Commerce ,Algorithms - Abstract
Makespan dominates the manufacturing expenses in bakery production. The high energy consumption of ovens also has a substantial impact, which bakers may overlook. Bakers leave ovens running until the final product is baked, allowing them to consume energy even when not in use. It results in energy waste, increased manufacturing costs, and CO2 emissions. This paper investigates three manufacturing lines from small and medium-sized bakeries to find optimum makespan and ovens’ idle time (OIDT). A hybrid no-wait flow shop scheduling model considering the constraints that are most common in bakeries is proposed. To find optimal solutions, non-dominated sorting genetic algorithm (NSGA-II), strength Pareto evolutionary algorithm (SPEA2), generalized differential evolution (GDE3), improved multi-objective particle swarm optimization (OMOPSO), and speed-constrained multi-objective particle swarm optimization (SMPSO) were used. The experimental results show that the shortest makespan does not always imply the lowest OIDT. Even the optimized solutions have up to 231 min of excess OIDT, while the makespan is the shortest. Pareto solutions provide promising trade-offs between makespan and OIDT, with the best-case scenario reducing OIDT by 1348 min while increasing makespan only by 61 min from the minimum possible makespan. NSGA-II outperforms all other algorithms in obtaining a high number of good-quality solutions and a small number of poor-quality solutions, followed by SPEA2 and GDE3. In contrast, OMOPSO and SMPSO deliver the worst solutions, which become pronounced as the problem complexity grows.
- Published
- 2022
16. Whimbrel populations differ in trans-atlantic pathways and cyclone encounters
- Author
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F M Smith, Bryan D. Watts, Paul F. Woodard, Jennie Rausch, Diana J. Hamilton, Julie Paquet, Laura Duval, Lisa Pirie-Dominix, Tim Keyes, Chance Hines, Barry R. Truitt, and Brad Winn
- Subjects
0106 biological sciences ,Delta ,Caribbean island ,Multidisciplinary ,010504 meteorology & atmospheric sciences ,Ecology ,Intertropical Convergence Zone ,Science ,Storm ,010603 evolutionary biology ,01 natural sciences ,Article ,Oceanography ,Geography ,Caribbean Basin ,Cyclone ,Medicine ,Tropical cyclone ,Bay ,Climate sciences ,0105 earth and related environmental sciences - Abstract
Each year hundreds of millions of birds cross the Atlantic Ocean during the peak of tropical cyclone activity. The extent and consequences of migrant-storm interactions remain unknown. We tracked whimbrels from two populations (Mackenzie Delta; Hudson Bay) to examine overlap between migration routes and storm activity and both the frequency and consequence of storm encounters. Here we show that Mackenzie Delta and Hudson Bay whimbrels follow different routes across the ocean and experience dramatically different rates of storm encounters. Mackenzie Delta whimbrels departed North America from Atlantic Canada, made long ($$\bar{x}$$ x ¯ = 5440 ± 120.3 km) nonstop flights far out to sea that took several days ($$\bar{x}$$ x ¯ = 6.1 ± 0.18) to complete and encountered storms during 3 of 22 crossings. Hudson Bay whimbrels departed North America from the south Atlantic Coast, made shorter ($$\bar{x}$$ x ¯ = 3643 ± 196.2 km) nonstop flights across the Caribbean Basin that took less time ($$\bar{x}$$ x ¯ = 4.5 ± 0.29) to complete and encountered storms during 13 of 18 crossings. More than half of Hudson Bay storm encounters resulted in groundings on Caribbean islands. Grounded birds required longer ($$\bar{x}$$ x ¯ = 30.4 ± 5.32 days) to complete trans-Atlantic crossings and three were lost including 2 to hunters and 1 to a predator. One of the Mackenzie Delta whimbrels was lost at sea while crossing the Intertropical Convergence Zone. Whimbrels use two contrasting strategies to cross the Atlantic including (1) a long nonstop flight around the core of storm activity with a low likelihood of encountering storms but no safety net and (2) a shorter flight through the heart of Hurricane Alley with a high likelihood of encountering storms and a safety network of islands to use in the event of an encounter. Demographic consequences of storm encounters will likely play a role in the ongoing evolution of trans-Atlantic migration pathways as global temperatures continue to rise.
- Published
- 2021
17. Masking terminal neo-epitopes of linear peptides through glycosylation favours immune responses towards core epitopes producing parental protein bound antibodies
- Author
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Amalia Ponce, Christine Gadoury, Anindita Chattopadhyay, Hongyan Zhou, Wei Zou, Dean Williams, Robert Pon, Anne Marcil, Eric Paquet, Komal Gurnani, Wangxue Chen, and Kenneth Chan
- Subjects
0301 basic medicine ,Immunogen ,Glycosylation ,Receptor, ErbB-2 ,Immunology ,Carbohydrates ,lcsh:Medicine ,Hemagglutinin (influenza) ,Neuraminidase ,Peptide ,Enzyme-Linked Immunosorbent Assay ,Hemagglutinin Glycoproteins, Influenza Virus ,Antibodies, Viral ,Epitope ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Epitopes ,Mice ,Viral Proteins ,0302 clinical medicine ,Animals ,Humans ,Biotinylation ,lcsh:Science ,chemistry.chemical_classification ,Mice, Inbred BALB C ,Multidisciplinary ,biology ,lcsh:R ,Biological techniques ,Glycopeptides ,Chemistry ,030104 developmental biology ,chemistry ,Biochemistry ,Influenza Vaccines ,030220 oncology & carcinogenesis ,Immune System ,Antibody Formation ,biology.protein ,lcsh:Q ,Female ,Antibody ,Dimerization - Abstract
Glycosylation of hydrophobic peptides at one terminus effectively increases their water-solubility, and conjugation through the opposing end to a carrier protein, renders them more immunogenic. Moreover, the glycosylation minimizes antibody responses to potentially deleterious, non-productive terminal neo-epitope regions of the peptides, and consequently shifts peptide immunogenicity towards the core amino acid residues. As proof of concept, glycopeptide-protein conjugates related to influenza hemagglutinin (HA), neuraminidase (NA), and the dimerization loop region of human epidermal growth factor receptor 2 (Her2), demonstrated a favorable production of core peptide specific antibodies as determined by ELISA studies. Furthermore, glycosylated Her2 peptide conjugate antisera were also shown to recognize full length Her2 protein by ELISA and at the cell surface through flow cytometry analysis. In contrast, unmasked peptide conjugates generated significant antibody populations that were specific to the terminal neo-epitope of the peptide immunogen that are notably absent in parental proteins. Antibodies generated in this manner to peptides in the dimerization loop of Her2 are also functional as demonstrated by the growth inhibition of Her2 expressing SKBR3 carcinoma cells. This method provides a technique to tailor-make epitope-specific antibodies that may facilitate vaccine, therapeutic and diagnostic antibody development.
- Published
- 2020
18. Spectroscopic analysis of chia seeds
- Author
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V. Zettel, Bernd Hitzmann, Monica Wanjiku Mburu, and Olivier Paquet-Durand
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Science ,01 natural sciences ,Characterization and analytical techniques ,Fluorescence spectroscopy ,Article ,Protein content ,0404 agricultural biotechnology ,Near-infrared spectroscopy ,Food science ,Spectroscopy ,Multidisciplinary ,Chemistry ,010401 analytical chemistry ,04 agricultural and veterinary sciences ,Nutritional information ,Standard normal variate ,040401 food science ,0104 chemical sciences ,South american ,Raman spectroscopy ,Medicine ,Nir spectra - Abstract
Chia seeds are becoming more and more popular in modern diets. In this contribution NIR and 2D-fluorescence spectroscopy were used to determine their nutritional values, mainly fat and protein content. 25 samples of chia seeds were analysed, whereof 9 samples were obtained from different regions in Kenya, 16 samples were purchased in stores in Germany and originated mostly from South America. For the purchased samples the nutritional information of the package was taken in addition to the values obtained for fat and protein, which were determined at the Hohenheim Core Facility. For the first time the NIR and fluorescence spectroscopy were used for the analysis of chia. For the spectral evaluation two different pre-processing methods were tested. Baseline correction with subsequent mean-centring lead to the best results for NIR spectra whereas SNV (standard normal variate transformation) was sufficient for the evaluation of fluorescence spectra. When combining NIR and fluorescence spectra, the fluorescence spectra were also multiplied with a factor to adjust the intensity levels. The best prediction results for the evaluation of the combined spectra were obtained for Kenyan samples with prediction errors below 0.2 g/100 g. For all other samples the absolute prediction error was 0.51 g/100 g for fat and 0.62 g/100 g for protein. It is possible to determine the amount of protein and fat of chia seeds by fluorescence and NIR spectroscopy. The combination of both methods is beneficial for the predictions. Chia seeds from Kenya had similar protein and lipid contents as South American seeds.
- Published
- 2020
19. Characterization and diversity of phages infecting Aeromonas salmonicida subsp. salmonicida
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Steve J. Charette, Antony T. Vincent, Alex Bernatchez, Sylvain Moineau, Valérie E. Paquet, and Denise M. Tremblay
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0301 basic medicine ,viruses ,animal diseases ,Science ,India ,Virulence ,Aeromonas salmonicida ,Genome, Viral ,Subspecies ,Genome ,Host Specificity ,Article ,Microbiology ,03 medical and health sciences ,Bacteriophages ,Selection, Genetic ,Gene ,Base Composition ,Multidisciplinary ,biology ,Genetic Variation ,biology.organism_classification ,030104 developmental biology ,Lytic cycle ,GenBank ,Codon usage bias ,Medicine - Abstract
Phages infecting Aeromonas salmonicida subsp. salmonicida, the causative agent of the fish disease furunculosis, have been isolated for decades but very few of them have been characterized. Here, the host range of 12 virulent phages, including three isolated in the present study, was evaluated against a panel of 65 A. salmonicida isolates, including representatives of the psychrophilic subspecies salmonicida, smithia, masoucida, and the mesophilic subspecies pectinolytica. This bacterial set also included three isolates from India suspected of being members of a new subspecies. Our results allowed to elucidate a lytic dichotomy based on the lifestyle of A. salmonicida (mesophilic or psychrophilic) and more generally, on phage types (lysotypes) for the subspecies salmonicida. The genomic analyses of the 12 phages from this study with those available in GenBank led us to propose an A. salmonicida phage pan-virome. Our comparative genomic analyses also suggest that some phage genes were under positive selection and A. salmonicida phage genomes having a discrepancy in GC% compared to the host genome encode tRNA genes to likely overpass the bias in codon usage. Finally, we propose a new classification scheme for A. salmonicida phages.
- Published
- 2017
20. Long-distance migratory shorebirds travel faster towards their breeding grounds, but fly faster post-breeding
- Author
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Stephanie Koch, Yves Aubry, David J. Newstead, Scott A. Flemming, Erica Nol, Cheri L. Gratto-Trevor, Rebecca L. Holberton, Christian Friis, Julie Paquet, Lawrence J. Niles, Paul Smith, Sjoerd Duijns, Ann E. McKellar, Lindsay Tudor, Charles M. Francis, Sarah G. Neima, Amanda Dey, Diana J. Hamilton, David S. Mizrahi, Alexandra M. Anderson, Jennie Rausch, Yves Turcotte, and Christy A. Morrissey
- Subjects
0301 basic medicine ,Multidisciplinary ,Reproductive success ,lcsh:R ,lcsh:Medicine ,Breeding ,Article ,Predation ,Birds ,Fishery ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Geography ,Flight, Animal ,Animals ,Telemetry ,lcsh:Q ,Animal Migration ,Seasons ,lcsh:Science ,Macroecology ,030217 neurology & neurosurgery ,Body condition - Abstract
Long-distance migrants are assumed to be more time-limited during the pre-breeding season compared to the post-breeding season. Although breeding-related time constraints may be absent post-breeding, additional factors such as predation risk could lead to time constraints that were previously underestimated. By using an automated radio telemetry system, we compared pre- and post-breeding movements of long-distance migrant shorebirds on a continent-wide scale. From 2014 to 2016, we deployed radio transmitters on 1,937 individuals of 4 shorebird species at 13 sites distributed across North America. Following theoretical predictions, all species migrated faster during the pre-breeding season, compared to the post-breeding season. These differences in migration speed between seasons were attributable primarily to longer stopover durations in the post-breeding season. In contrast, and counter to our expectations, all species had higher airspeeds during the post-breeding season, even after accounting for seasonal differences in wind. Arriving at the breeding grounds in good body condition is beneficial for survival and reproductive success and this energetic constraint might explain why airspeeds are not maximised in the pre-breeding season. We show that the higher airspeeds in the post-breeding season precede a wave of avian predators, which could suggest that migrant shorebirds show predation-minimizing behaviour during the post-breeding season. Our results reaffirm the important role of time constraints during northward migration and suggest that both energy and predation-risk constrain migratory behaviour during the post-breeding season.
- Published
- 2019
21. New protein-DNA complexes in archaea: a small monomeric protein induces a sharp V-turn DNA structure
- Author
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Karine Loth, Céline Landon, Agnès F. Delmas, Justine Largillière, Françoise Paquet, Bertrand Castaing, Franck Coste, Françoise Culard, Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Loth, Karine, Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), LARGILLIERE, Justine, Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université d'Orléans (UO)
- Subjects
0301 basic medicine ,Models, Molecular ,Cell division ,Protein Conformation ,[SDV]Life Sciences [q-bio] ,lcsh:Medicine ,chemistry.chemical_compound ,Transcription (biology) ,lcsh:Science ,ComputingMilieux_MISCELLANEOUS ,Genomic organization ,Multidisciplinary ,biology ,[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM] ,Monomer ,[SDV] Life Sciences [q-bio] ,DNA-Binding Proteins ,DNA, Archaeal ,Ribonucleoproteins ,Methanosarcina ,Protein Binding ,[CHIM.ANAL] Chemical Sciences/Analytical chemistry ,[SDV.BBM.BS] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM] ,Base pair ,Archaeal Proteins ,030106 microbiology ,Biophysics ,Dihedral angle ,Article ,03 medical and health sciences ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Nuclear Magnetic Resonance, Biomolecular ,lcsh:R ,DNA ,biology.organism_classification ,Archaea ,030104 developmental biology ,chemistry ,Nucleic Acid Conformation ,lcsh:Q ,Solution-state NMR - Abstract
MC1, a monomeric nucleoid-associated protein (NAP), is structurally unrelated to other DNA-binding proteins. The protein participates in the genome organization of several Euryarchaea species through an atypical compaction mechanism. It is also involved in DNA transcription and cellular division through unknown mechanisms. We determined the 3D solution structure of a new DNA-protein complex formed by MC1 and a strongly distorted 15 base pairs DNA. While the protein just needs to adapt its conformation slightly, the DNA undergoes a dramatic curvature (the first two bend angles of 55° and 70°, respectively) and an impressive torsional stress (dihedral angle of 106°) due to several kinks upon binding of MC1 to its concave side. Thus, it adopts a V-turn structure. For longer DNAs, MC1 stabilizes multiple V-turn conformations in a flexible and dynamic manner. The existence of such V-turn conformations of the MC1-DNA complexes leads us to propose two binding modes of the protein, as a bender (primary binding mode) and as a wrapper (secondary binding mode). Moreover, it opens up new opportunities for studying and understanding the repair, replication and transcription molecular machineries of Archaea.
- Published
- 2019
22. The paracrine effects of human induced pluripotent stem cells promote bone-like structures via the upregulation of BMP expression in a mouse ectopic model
- Author
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Hervé Petite, Karim Oudina, Nathanael Larochette, Christian Pinset, Mickael Deschepper, Emmanuelle Massouridès, Morad Bensidhoum, Joseph Paquet, and Adrien Moya
- Subjects
0301 basic medicine ,Cellular differentiation ,Induced Pluripotent Stem Cells ,Paracrine Communication ,lcsh:Medicine ,Regenerative Medicine ,Regenerative medicine ,Article ,Extracellular matrix ,03 medical and health sciences ,Paracrine signalling ,Mice ,0302 clinical medicine ,Tissue engineering ,Osteogenesis ,Animals ,Humans ,lcsh:Science ,Cells, Cultured ,Multidisciplinary ,biology ,Tissue Engineering ,Chemistry ,lcsh:R ,Mesenchymal stem cell ,Cell Differentiation ,Mesenchymal Stem Cells ,Cell biology ,Up-Regulation ,030104 developmental biology ,Gene Expression Regulation ,Culture Media, Conditioned ,Bone Morphogenetic Proteins ,Osteocalcin ,biology.protein ,lcsh:Q ,Female ,030217 neurology & neurosurgery - Abstract
Use of human induced pluripotent stem cells (h-iPSCs) for bone tissue engineering is most appealing, because h-iPSCs are an inexhaustible source of osteocompetent cells. The present study investigated the contribution of undifferentiated h-iPSCs and elucidated aspects of the underlying mechanism(s) of the involvement of these cells to new bone formation. Implantation of undifferentiated h-iPSCs seeded on coral particles in ectopic sites of mice resulted in expression of osteocalcin and DMP-1, and in mineral content similar to that of the murine bone. The number of the implanted h-iPSCs decreased with time and disappeared by 30 days post-implantation. In contrast, expression of the murine osteogenic genes at day 15 and 30 post-implantation provided, for the first time, evidence that the implanted h-iPSCs affected the observed outcomes via paracrine mechanisms. Supporting evidence was provided because supernatant conditioned media from h-iPSCs (h-iPSC CM), promoted the osteogenic differentiation of human mesenchymal stem cells (h-MSCs) in vitro. Specifically, h-iPSC CM induced upregulation of the BMP-2, BMP-4 and BMP-6 genes, and promoted mineralization of the extracellular matrix. Given the current interest in the use of h-iPSCs for regenerative medicine applications, our study contributes new insights into aspects of the mechanism underlying the bone promoting capability of h-iPSCs.
- Published
- 2018
23. Evaluating anthropogenic threats to endangered killer whales to inform effective recovery plans
- Author
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Lauren J. N. Brent, Deborah A. Giles, Christopher W. Clark, Erin Ashe, Kenneth C. Balcomb, Paul C. Paquet, Darren P. Croft, Robert C. Lacy, Misty MacDuffee, and Rob Williams
- Subjects
0106 biological sciences ,Conservation of Natural Resources ,Population ,Endangered species ,lcsh:Medicine ,010603 evolutionary biology ,01 natural sciences ,Article ,Critically endangered ,Animals ,Population growth ,Human Activities ,lcsh:Science ,education ,education.field_of_study ,Multidisciplinary ,Ecology ,010604 marine biology & hydrobiology ,Endangered Species ,lcsh:R ,Cumulative effects ,Population viability analysis ,Disturbance (ecology) ,Environmental science ,lcsh:Q ,Whale, Killer ,Conservation biology - Abstract
Understanding cumulative effects of multiple threats is key to guiding effective management to conserve endangered species. The critically endangered, Southern Resident killer whale population of the northeastern Pacific Ocean provides a data-rich case to explore anthropogenic threats on population viability. Primary threats include: limitation of preferred prey, Chinook salmon; anthropogenic noise and disturbance, which reduce foraging efficiency; and high levels of stored contaminants, including PCBs. We constructed a population viability analysis to explore possible demographic trajectories and the relative importance of anthropogenic stressors. The population is fragile, with no growth projected under current conditions, and decline expected if new or increased threats are imposed. Improvements in fecundity and calf survival are needed to reach a conservation objective of 2.3% annual population growth. Prey limitation is the most important factor affecting population growth. However, to meet recovery targets through prey management alone, Chinook abundance would have to be sustained near the highest levels since the 1970s. The most optimistic mitigation of noise and contaminants would make the difference between a declining and increasing population, but would be insufficient to reach recovery targets. Reducing acoustic disturbance by 50% combined with increasing Chinook by 15% would allow the population to reach 2.3% growth.
- Published
- 2017
24. A retinal model of cerebral malaria
- Author
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François Paquet-Durand, Sylvia Bolz, Benjamin Mordmüller, Marina Garcia-Garrido, Naoyuki Tanimoto, Susanne C. Beck, Wolfgang Hoffmann, Regine Mühlfriedel, Ulrich Schraermeyer, Mathias W. Seeliger, Soumyaparna Das, Le Chang, Timm Schubert, and Gesine Huber
- Subjects
0301 basic medicine ,Plasmodium berghei ,medicine.medical_treatment ,Malaria, Cerebral ,lcsh:Medicine ,Dihydroartemisinin ,Gene Expression ,Mice, Transgenic ,Disease ,Retina ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Genes, Reporter ,parasitic diseases ,medicine ,Electroretinography ,Animals ,lcsh:Science ,Cellular localization ,Multidisciplinary ,biology ,business.industry ,lcsh:R ,Retinal ,medicine.disease ,biology.organism_classification ,Ophthalmoscopy ,Disease Models, Animal ,030104 developmental biology ,Phenotype ,chemistry ,Cerebral Malaria ,Immunology ,lcsh:Q ,business ,030217 neurology & neurosurgery ,Malaria ,Biomarkers ,Tomography, Optical Coherence ,Retinopathy - Abstract
Malaria is a causative factor in about 500.000 deaths each year world-wide. Cerebral malaria is a particularly severe complication of this disease and thus associated with an exceedingly high mortality. Malaria retinopathy is an ocular manifestation often associated with cerebral malaria, and presumably shares a substantial part of its pathophysiology. Here, we describe that indeed murine malaria retinopathy reproduced the main hallmarks of the corresponding human disease. In the living animal, we were able to follow the circulation and cellular localization of malaria parasites transgenically labelled with GFP via non-invasive in vivo retinal imaging. We found that malaria parasites cross the blood-retinal-barrier and infiltrate the neuroretina, concomitant with an extensive, irreversible, and long-lasting retinal neurodegeneration. Furthermore, anti-malarial treatment with dihydroartemisinin strongly diminished the load of circulating parasites but resolved the symptoms of the retinopathy only in part. In summary, we introduce here a novel preclinical model for human cerebral malaria that is much more directly accessible for studies into disease pathophysiology and development of novel treatment approaches. In vivo retinal imaging may furthermore serve as a valuable tool for the early diagnosis of the human disease.
- Published
- 2017
25. Olaparib significantly delays photoreceptor loss in a model for hereditary retinal degeneration
- Author
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Sahaboglu, Ayse, Barth, Melanie, Secer, Enver, del Amo, Eva M., Urtti, Arto, Arsenijevic, Yvan, Zrenner, Eberhart, Paquet-Durand, Francois, Faculty of Pharmacy, Division of Pharmaceutical Biosciences, Drug Delivery, Drug Research Program, and Drug Delivery Unit
- Subjects
ROD CGMP-PHOSPHODIESTERASE ,Cell Survival ,Quantitative Structure-Activity Relationship ,POLY(ADP-RIBOSE) POLYMERASE ,Poly(ADP-ribose) Polymerase Inhibitors ,PIGMENTOSA ,Article ,Piperazines ,ACTIVATION ,Mice ,Neoplasms ,Animals ,BETA-SUBUNIT ,Cyclic GMP ,MOUSE RETINA ,Mice, Inbred C3H ,Retinal Degeneration ,PARP INHIBITORS ,3112 Neurosciences ,1184 Genetics, developmental biology, physiology ,THERAPEUTIC OPPORTUNITIES ,Immunohistochemistry ,Chromatin ,eye diseases ,Neuroprotective Agents ,DNA-DAMAGE ,CELL-DEATH ,317 Pharmacy ,Phthalazines ,Rabbits ,sense organs ,Poly(ADP-ribose) Polymerases ,Photoreceptor Cells, Vertebrate ,Protein Binding - Abstract
The enzyme poly-ADP-ribose-polymerase (PARP) mediates DNA-repair and rearrangements of the nuclear chromatin. Generally, PARP activity is thought to promote cell survival and in recent years a number of PARP inhibitors have been clinically developed for cancer treatment. Paradoxically, PARP activity is also connected to many diseases including the untreatable blinding disease Retinitis Pigmentosa (RP), where PARP activity appears to drive the pathogenesis of photoreceptor loss. We tested the efficacy of three different PARP inhibitors to prevent photoreceptor loss in the rd1 mouse model for RP. In retinal explant cultures in vitro, olaparib had strong and long-lasting photoreceptor neuroprotective capacities. We demonstrated target engagement by showing that olaparib reduced photoreceptor accumulation of poly-ADP-ribosylated proteins. Remarkably, olaparib also reduced accumulation of cyclic-guanosine-monophosphate (cGMP), a characteristic marker for photoreceptor degeneration. Moreover, intravitreal injection of olaparib in rd1 animals diminished PARP activity and increased photoreceptor survival, confirming in vivo neuroprotection. This study affirms the role of PARP in inherited retinal degeneration and for the first time shows that a clinically approved PARP inhibitor can prevent photoreceptor degeneration in an RP model. The wealth of human clinical data available for olaparib highlights its strong potential for a rapid clinical translation into a novel RP treatment.
- Published
- 2016
- Full Text
- View/download PDF
26. Loss of mesenchymal bone morphogenetic protein signaling leads to development of reactive stroma and initiation of the gastric neoplastic cascade
- Author
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Véronique Pomerleau, Camille Ouellet, Sébastien A. B. Roy, Jasmin Rousseau, Perrine Garde-Granger, Etienne Lemieux, François Boudreau, Jean-Philippe Babeu, Nathalie Perreault, Marilène Paquet, Faiza Maloum-Rami, and Joannie M. Allaire
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,animal structures ,Stromal cell ,Cellular differentiation ,Mesenchyme ,Population ,Biology ,Bone morphogenetic protein ,Article ,Adenomatous Polyps ,Mice ,03 medical and health sciences ,Stomach Neoplasms ,medicine ,Animals ,education ,Bone Morphogenetic Protein Receptors, Type I ,Cell Proliferation ,education.field_of_study ,Multidisciplinary ,digestive, oral, and skin physiology ,Transdifferentiation ,Cell Differentiation ,digestive system diseases ,BMPR1A ,BMPR2 ,Cell biology ,Gene Expression Regulation, Neoplastic ,Cell Transformation, Neoplastic ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,Bone Morphogenetic Proteins ,embryonic structures ,Stromal Cells ,Signal Transduction - Abstract
Bmps are morphogens involved in various gastric cellular functions. Studies in genetically-modified mice have shown that Bmp disruption in gastric epithelial and stromal cell compartments leads to the development of tumorigenesis. Our studies have demonstrated that abrogation of gastric epithelial Bmp signaling alone was not sufficient to recapitulate the neoplastic features associated with total gastric loss of Bmp signaling. Thus, epithelial Bmp signaling does not appear to be a key player in gastric tumorigenesis initiation. These observations suggest a greater role for stromal Bmp signaling in gastric polyposis initiation. In order to identify the specific roles played by mesenchymal Bmp signaling in gastric homeostasis, we generated a mouse model with abrogation of Bmp signaling exclusively in the gastro-intestinal mesenchyme (Bmpr1aΔMES). We were able to expose an unsuspected role for Bmp loss of signaling in leading normal gastric mesenchyme to adapt into reactive mesenchyme. An increase in the population of activated-fibroblasts, suggesting mesenchymal transdifferentiation, was observed in mutant stomach. Bmpr1aΔMES stomachs exhibited spontaneous benign polyps with presence of both intestinal metaplasia and spasmolytic-polypeptide-expressing metaplasia as early as 90 days postnatal. These results support the novel concept that loss of mesenchymal Bmp signaling cascade acts as a trigger in gastric polyposis initiation.
- Published
- 2016
27. hSSB1 (NABP2/OBFC2B) is regulated by oxidative stress
- Author
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Emma Bolderson, Derek J. Richard, Catherine H. Botting, Liza Cubeddu, Nicolas Paquet, Sam Beard, Vincent Leong, Mark N. Adams, Kenneth J. O'Byrne, Christine Touma, Nicholas W. Ashton, Roland Gamsjaeger, University of St Andrews. School of Biology, University of St Andrews. School of Chemistry, University of St Andrews. EaSTCHEM, and University of St Andrews. Biomedical Sciences Research Complex
- Subjects
0301 basic medicine ,DNA Repair ,DNA repair ,DNA damage ,QH301 Biology ,NDAS ,Biology ,Article ,Mitochondrial Proteins ,QH301 ,03 medical and health sciences ,Biopolymers ,SDG 3 - Good Health and Well-being ,Humans ,QD ,Amino Acid Sequence ,Replication protein A ,Multidisciplinary ,Sequence Homology, Amino Acid ,030102 biochemistry & molecular biology ,Base excision repair ,DNA repair protein XRCC4 ,QD Chemistry ,Cell biology ,DNA-Binding Proteins ,Oxidative Stress ,030104 developmental biology ,Biochemistry ,DNA glycosylase ,Homologous recombination ,Dimerization ,DNA Damage ,Nucleotide excision repair - Abstract
The maintenance of genome stability is an essential cellular process to prevent the development of diseases including cancer. hSSB1 (NABP2/ OBFC2A) is a critical component of the DNA damage response where it participates in the repair of double-strand DNA breaks and in base excision repair of oxidized guanine residues (8-oxoguanine) by aiding the localization of the human 8-oxoguanine glycosylase (hOGG1) to damaged DNA. Here we demonstrate that following oxidative stress, hSSB1 is stabilized as an oligomer which is required for hSSB1 to function in the removal of 8-oxoguanine. Monomeric hSSB1 shows a decreased affinity for oxidized DNA resulting in a cellular 8-oxoguanine-repair defect and in the absence of ATM signaling initiation. While hSSB1 oligomerization is important for the removal of 8-oxoguanine from the genome, it is not required for the repair of double-strand DNA-breaks by homologous recombination. These findings demonstrate a novel hSSB1 regulatory mechanism for the repair of damaged DNA.
- Published
- 2016
28. Ecology of conflict: marine food supply affects human-wildlife interactions on land
- Author
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Paul C. Paquet, Andrew B. Cooper, Chris T. Darimont, Sean C. Anderson, Kyle A. Artelle, and John D. Reynolds
- Subjects
0106 biological sciences ,Resource (biology) ,Population ,Wildlife ,Animals, Wild ,010603 evolutionary biology ,01 natural sciences ,Article ,Food Supply ,Salmon ,Animals ,Humans ,Ecosystem ,14. Life underwater ,education ,education.field_of_study ,Biomass (ecology) ,Multidisciplinary ,Behavior, Animal ,British Columbia ,biology ,Ecology ,010604 marine biology & hydrobiology ,biology.organism_classification ,Carnivory ,Harm ,13. Climate action ,Oncorhynchus ,Conservation biology ,Ursidae - Abstract
Human-wildlife conflicts impose considerable costs to people and wildlife worldwide. Most research focuses on proximate causes, offering limited generalizable understanding of ultimate drivers. We tested three competing hypotheses (problem individuals, regional population saturation, limited food supply) that relate to underlying processes of human-grizzly bear (Ursus arctos horribilis) conflict, using data from British Columbia, Canada, between 1960–2014. We found most support for the limited food supply hypothesis: in bear populations that feed on spawning salmon (Oncorhynchus spp.), the annual number of bears/km2 killed due to conflicts with humans increased by an average of 20% (6–32% [95% CI]) for each 50% decrease in annual salmon biomass. Furthermore, we found that across all bear populations (with or without access to salmon), 81% of attacks on humans and 82% of conflict kills occurred after the approximate onset of hyperphagia (July 1st), a period of intense caloric demand. Contrary to practices by many management agencies, conflict frequency was not reduced by hunting or removal of problem individuals. Our finding that a marine resource affects terrestrial conflict suggests that evidence-based policy for reducing harm to wildlife and humans requires not only insight into ultimate drivers of conflict, but also management that spans ecosystem and jurisdictional boundaries.
- Published
- 2016
29. Evaluating anthropogenic threats to endangered killer whales to inform effective recovery plans
- Author
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Lacy, Robert C., primary, Williams, Rob, additional, Ashe, Erin, additional, Balcomb III, Kenneth C., additional, Brent, Lauren J. N., additional, Clark, Christopher W., additional, Croft, Darren P., additional, Giles, Deborah A., additional, MacDuffee, Misty, additional, and Paquet, Paul C., additional
- Published
- 2017
- Full Text
- View/download PDF
30. Characterization and diversity of phages infecting Aeromonas salmonicida subsp. salmonicida
- Author
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Vincent, Antony T., primary, Paquet, Valérie E., additional, Bernatchez, Alex, additional, Tremblay, Denise M., additional, Moineau, Sylvain, additional, and Charette, Steve J., additional
- Published
- 2017
- Full Text
- View/download PDF
31. The human decapping scavenger enzyme DcpS modulates microRNA turnover
- Author
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Michael A. Tones, Sandra Piquet, Eric Paquet, Gabriel D. Bossé, Oussama Meziane, Claude Robert, Martin J. Simard, and Dominic Gagné
- Subjects
Exonuclease ,RNA Caps ,DCPS ,Blotting, Western ,Molecular Sequence Data ,Active Transport, Cell Nucleus ,Fluorescent Antibody Technique ,Biology ,medicine.disease_cause ,Article ,RNA interference ,microRNA ,Endoribonucleases ,medicine ,Gene silencing ,Humans ,Amino Acid Sequence ,Peptide sequence ,Oligonucleotide Array Sequence Analysis ,Cell Nucleus ,Mutation ,Multidisciplinary ,Sequence Homology, Amino Acid ,Reverse Transcriptase Polymerase Chain Reaction ,Gene Expression Profiling ,HEK 293 cells ,Blotting, Northern ,Molecular biology ,Cell biology ,MicroRNAs ,HEK293 Cells ,biology.protein ,RNA Interference - Abstract
The decapping scavenger enzyme DcpS is known for its role in hydrolyzing the cap structure following mRNA degradation. Recently, we discovered a new function in miRNA degradation activation for the ortholog of DcpS in C. elegans. Here we show that human DcpS conserves its role in miRNA turnover. In human cells, DcpS is a nucleocytoplasmic shuttling protein that activates miRNA degradation independently of its scavenger decapping activity in the cytoplasmic compartment. We also demonstrate that this new function for DcpS requires the contribution of the 5′-3′ exonuclease Xrn2. Our findings support a conserved role of DcpS as a modulator of miRNA turnover in animals.
- Published
- 2015
32. Drug Delivery by Tattooing to Treat Cutaneous Leishmaniasis
- Author
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Anny Fortin, Martin Olivier, Tom Bosschaerts, Stef Stienstra, Marina Temi Shio, Caroline Martel, and Marilène Paquet
- Subjects
Drug ,Pathology ,medicine.medical_specialty ,media_common.quotation_subject ,Leishmaniasis, Cutaneous ,Article ,Cell Line ,Mice ,Cutaneous leishmaniasis ,In vivo ,medicine ,Animals ,media_common ,Leishmania ,Mice, Inbred BALB C ,Multidisciplinary ,Tattooing ,biology ,business.industry ,Macrophages ,Leishmaniasis ,medicine.disease ,biology.organism_classification ,Regimen ,Liposomes ,Immunology ,Drug delivery ,Phosphatidylcholines ,Female ,Histopathology ,business - Abstract
This study establishes a proof-of-concept that a tattoo device can target intra-dermal drug delivery against cutaneous leishmaniasis (CL). The selected drug is oleylphosphocholine (OlPC) formulated as liposomes, particles known to be prone to macrophage ingestion. We first show that treatment of cultured Leishmania-infected macrophages with OlPC-liposomes results in a direct dose-dependent killing of intracellular parasites. Based on this, in vivo efficacy is demonstrated using a 10 day tattooing-mediated treatment in mice infected with L. major and L. mexicana. In both models this regimen results in rapid clinical recovery with complete regression of skin lesions by Day 28. Parasite counts and histopathology examination confirm high treatment efficacy at the parasitic level. Low amount of drug required for tattooing combined with fast clinical recovery may have a positive impact on CL patient management. This first example of tattoo-mediated drug delivery could open to new therapeutic interventions in the treatment of skin diseases.
- Published
- 2014
33. A new paradigm for transcription factor TFIIB functionality
- Author
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Janice M. Zabolotny, Dimcho Bachvarov, Sang Wook Yoo, Martin Lange, Makoto Hiromura, Anna S. Kushnir, Vladimir Gelev, Anny Usheva, Eric Paquet, Priscilla A. Schaffer, Nobuo Horikoshi, and Joseph S. Orlando
- Subjects
Gene Expression Regulation, Viral ,Transcription, Genetic ,Datasets as Topic ,Gene Expression ,RNA polymerase II ,Herpesvirus 1, Human ,Article ,Cell Line ,Transcription (biology) ,Gene expression ,Gene Knockdown Techniques ,Gene silencing ,Animals ,Humans ,Gene Silencing ,Genetics ,Regulation of gene expression ,Multidisciplinary ,Binding Sites ,biology ,Genome, Human ,Gene Expression Profiling ,Cell Cycle ,Promoter ,Acetylation ,enzymes and coenzymes (carbohydrates) ,Gene Expression Regulation ,Organ Specificity ,biology.protein ,Transcription Factor TFIIB ,Genes, Lethal ,RNA Polymerase II ,Transcription Initiation Site ,Transcriptome ,Transcription factor II B ,Protein Binding - Abstract
Experimental and bioinformatic studies of transcription initiation by RNA polymerase II (RNAP2) have revealed a mechanism of RNAP2 transcription initiation less uniform across gene promoters than initially thought. However, the general transcription factor TFIIB is presumed to be universally required for RNAP2 transcription initiation. Based on bioinformatic analysis of data and effects of TFIIB knockdown in primary and transformed cell lines on cellular functionality and global gene expression, we report that TFIIB is dispensable for transcription of many human promoters, but is essential for herpes simplex virus-1 (HSV-1) gene transcription and replication. We report a novel cell cycle TFIIB regulation and localization of the acetylated TFIIB variant on the transcriptionally silent mitotic chromatids. Taken together, these results establish a new paradigm for TFIIB functionality in human gene expression, which when downregulated has potent anti-viral effects.
- Published
- 2014
34. Loss of mesenchymal bone morphogenetic protein signaling leads to development of reactive stroma and initiation of the gastric neoplastic cascade
- Author
-
Roy, Sébastien A. B., primary, Allaire, Joannie M., additional, Ouellet, Camille, additional, Maloum-Rami, Faiza, additional, Pomerleau, Véronique, additional, Lemieux, Étienne, additional, Babeu, Jean-Philippe, additional, Rousseau, Jasmin, additional, Paquet, Marilène, additional, Garde-Granger, Perrine, additional, Boudreau, François, additional, and Perreault, Nathalie, additional
- Published
- 2016
- Full Text
- View/download PDF
35. hSSB1 (NABP2/OBFC2B) is regulated by oxidative stress
- Author
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Paquet, Nicolas, primary, Adams, Mark N., additional, Ashton, Nicholas W., additional, Touma, Christine, additional, Gamsjaeger, Roland, additional, Cubeddu, Liza, additional, Leong, Vincent, additional, Beard, Sam, additional, Bolderson, Emma, additional, Botting, Catherine H., additional, O’Byrne, Kenneth J., additional, and Richard, Derek J., additional
- Published
- 2016
- Full Text
- View/download PDF
36. Ecology of conflict: marine food supply affects human-wildlife interactions on land
- Author
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Artelle, Kyle A., primary, Anderson, Sean C., additional, Reynolds, John D., additional, Cooper, Andrew B., additional, Paquet, Paul C., additional, and Darimont, Chris T., additional
- Published
- 2016
- Full Text
- View/download PDF
37. The human decapping scavenger enzyme DcpS modulates microRNA turnover
- Author
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Meziane, Oussama, primary, Piquet, Sandra, additional, Bossé, Gabriel D., additional, Gagné, Dominic, additional, Paquet, Eric, additional, Robert, Claude, additional, Tones, Michael A., additional, and Simard, Martin J., additional
- Published
- 2015
- Full Text
- View/download PDF
38. Neuroinflammation and Aβ Accumulation Linked To Systemic Inflammation Are Decreased By Genetic PKR Down-Regulation
- Author
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Carret-Rebillat, Anne-Sophie, primary, Pace, Clarisse, additional, Gourmaud, Sarah, additional, Ravasi, Laura, additional, Montagne-Stora, Samantha, additional, Longueville, Sophie, additional, Tible, Marion, additional, Sudol, Erika, additional, Chang, Raymond Chuen-Chung, additional, Paquet, Claire, additional, Mouton-Liger, François, additional, and Hugon, Jacques, additional
- Published
- 2015
- Full Text
- View/download PDF
39. Drug Delivery by Tattooing to Treat Cutaneous Leishmaniasis
- Author
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Shio, Marina Temi, primary, Paquet, Marilene, additional, Martel, Caroline, additional, Bosschaerts, Tom, additional, Stienstra, Stef, additional, Olivier, Martin, additional, and Fortin, Anny, additional
- Published
- 2014
- Full Text
- View/download PDF
40. A new paradigm for transcription factor TFIIB functionality
- Author
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Gelev, Vladimir, primary, Zabolotny, Janice M., additional, Lange, Martin, additional, Hiromura, Makoto, additional, Yoo, Sang Wook, additional, Orlando, Joseph S., additional, Kushnir, Anna, additional, Horikoshi, Nobuo, additional, Paquet, Eric, additional, Bachvarov, Dimcho, additional, Schaffer, Priscilla A., additional, and Usheva, Anny, additional
- Published
- 2014
- Full Text
- View/download PDF
41. Whimbrel populations differ in trans-atlantic pathways and cyclone encounters
- Author
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Bryan D. Watts, Fletcher M. Smith, Chance Hines, Laura Duval, Diana J. Hamilton, Tim Keyes, Julie Paquet, Lisa Pirie-Dominix, Jennie Rausch, Barry Truitt, Brad Winn, and Paul Woodard
- Subjects
Medicine ,Science - Abstract
Abstract Each year hundreds of millions of birds cross the Atlantic Ocean during the peak of tropical cyclone activity. The extent and consequences of migrant-storm interactions remain unknown. We tracked whimbrels from two populations (Mackenzie Delta; Hudson Bay) to examine overlap between migration routes and storm activity and both the frequency and consequence of storm encounters. Here we show that Mackenzie Delta and Hudson Bay whimbrels follow different routes across the ocean and experience dramatically different rates of storm encounters. Mackenzie Delta whimbrels departed North America from Atlantic Canada, made long ( $$\bar{x}$$ x ¯ = 5440 ± 120.3 km) nonstop flights far out to sea that took several days ( $$\bar{x}$$ x ¯ = 6.1 ± 0.18) to complete and encountered storms during 3 of 22 crossings. Hudson Bay whimbrels departed North America from the south Atlantic Coast, made shorter ( $$\bar{x}$$ x ¯ = 3643 ± 196.2 km) nonstop flights across the Caribbean Basin that took less time ( $$\bar{x}$$ x ¯ = 4.5 ± 0.29) to complete and encountered storms during 13 of 18 crossings. More than half of Hudson Bay storm encounters resulted in groundings on Caribbean islands. Grounded birds required longer ( $$\bar{x}$$ x ¯ = 30.4 ± 5.32 days) to complete trans-Atlantic crossings and three were lost including 2 to hunters and 1 to a predator. One of the Mackenzie Delta whimbrels was lost at sea while crossing the Intertropical Convergence Zone. Whimbrels use two contrasting strategies to cross the Atlantic including (1) a long nonstop flight around the core of storm activity with a low likelihood of encountering storms but no safety net and (2) a shorter flight through the heart of Hurricane Alley with a high likelihood of encountering storms and a safety network of islands to use in the event of an encounter. Demographic consequences of storm encounters will likely play a role in the ongoing evolution of trans-Atlantic migration pathways as global temperatures continue to rise.
- Published
- 2021
- Full Text
- View/download PDF
42. Spectroscopic analysis of chia seeds
- Author
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Monica Mburu, Olivier Paquet-Durand, Bernd Hitzmann, and Viktoria Zettel
- Subjects
Medicine ,Science - Abstract
Abstract Chia seeds are becoming more and more popular in modern diets. In this contribution NIR and 2D-fluorescence spectroscopy were used to determine their nutritional values, mainly fat and protein content. 25 samples of chia seeds were analysed, whereof 9 samples were obtained from different regions in Kenya, 16 samples were purchased in stores in Germany and originated mostly from South America. For the purchased samples the nutritional information of the package was taken in addition to the values obtained for fat and protein, which were determined at the Hohenheim Core Facility. For the first time the NIR and fluorescence spectroscopy were used for the analysis of chia. For the spectral evaluation two different pre-processing methods were tested. Baseline correction with subsequent mean-centring lead to the best results for NIR spectra whereas SNV (standard normal variate transformation) was sufficient for the evaluation of fluorescence spectra. When combining NIR and fluorescence spectra, the fluorescence spectra were also multiplied with a factor to adjust the intensity levels. The best prediction results for the evaluation of the combined spectra were obtained for Kenyan samples with prediction errors below 0.2 g/100 g. For all other samples the absolute prediction error was 0.51 g/100 g for fat and 0.62 g/100 g for protein. It is possible to determine the amount of protein and fat of chia seeds by fluorescence and NIR spectroscopy. The combination of both methods is beneficial for the predictions. Chia seeds from Kenya had similar protein and lipid contents as South American seeds.
- Published
- 2021
- Full Text
- View/download PDF
43. Characterization and diversity of phages infecting Aeromonas salmonicida subsp. salmonicida
- Author
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Antony T. Vincent, Valérie E. Paquet, Alex Bernatchez, Denise M. Tremblay, Sylvain Moineau, and Steve J. Charette
- Subjects
Medicine ,Science - Abstract
Abstract Phages infecting Aeromonas salmonicida subsp. salmonicida, the causative agent of the fish disease furunculosis, have been isolated for decades but very few of them have been characterized. Here, the host range of 12 virulent phages, including three isolated in the present study, was evaluated against a panel of 65 A. salmonicida isolates, including representatives of the psychrophilic subspecies salmonicida, smithia, masoucida, and the mesophilic subspecies pectinolytica. This bacterial set also included three isolates from India suspected of being members of a new subspecies. Our results allowed to elucidate a lytic dichotomy based on the lifestyle of A. salmonicida (mesophilic or psychrophilic) and more generally, on phage types (lysotypes) for the subspecies salmonicida. The genomic analyses of the 12 phages from this study with those available in GenBank led us to propose an A. salmonicida phage pan-virome. Our comparative genomic analyses also suggest that some phage genes were under positive selection and A. salmonicida phage genomes having a discrepancy in GC% compared to the host genome encode tRNA genes to likely overpass the bias in codon usage. Finally, we propose a new classification scheme for A. salmonicida phages.
- Published
- 2017
- Full Text
- View/download PDF
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