1. Synthesis and Antibacterial Activities of Antibacterial Peptides with a Spiropyran Fluorescence Probe.
- Author
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Lei Chen, Yu Zhu, Danling Yang, Rongfeng Zou, Junchen Wu, and He Tian
- Subjects
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ANTIBACTERIAL agents , *PEPTIDES , *SPIROPYRANS , *FLUORESCENCE yield , *MEROCYANINES , *ISOMERS - Abstract
In this report, antibacterial peptides1-3 were prepared with a spiropyran fluorescence probe. The probe exhibits a change in fluorescence when isomerized from a colorless spiro-form (spiropyran, Sp) to a colored open-form (merocyanine, Mc) under different chemical environments, which can be used to study the mechanism of antimicrobial activity. Peptides 1-3 exhibit a marked decrease in antimicrobial activity with increasing alkyl chain length. This is likely due to the Sp-Mc isomers in different polar environments forming different aggregate sizes in TBS, as demonstrated by time-dependent dynamic light scattering (DLS). Moreover, peptides 1-3 exhibited low cytotoxicity and hemolytic activity. These probe-modified peptides may provide a novel approach to study the effect of structural changes on antibacterial activity, thus facilitating the design of new antimicrobial agents to combat bacterial infection. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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