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302 results on '"Institut de Chimie du CNRS (INC)"'

1. A proof of principle study using radiopharmaceuticals to quantify and localize container-content interactions in medical syringes

Author: Dupire, C., Chennell, P., Pereira, B., Courtiol, N., Buj, S., Cueff, R., Crauste-Manciet, S., Sautou, V., Morgat, Clement, Institut de Chimie de Clermont-Ferrand (ICCF), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Micro et Nanomédecines Translationnelles (MINT), Université d'Angers (UA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de Neurosciences cognitives et intégratives d'Aquitaine (INCIA), and Université Bordeaux Segalen - Bordeaux 2-Université Sciences et Technologies - Bordeaux 1 (UB)-SFR Bordeaux Neurosciences-Centre National de la Recherche Scientifique (CNRS)
Subjects: Multidisciplinary, Syringes, Rubber, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, [PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph], Radiopharmaceuticals, [SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences
Abstract: The sorption of drugs onto their contents is a known phenomenon that is difficult to analyse precisely. The purpose of this study was to present a non-invasive method for locating and quantifying sorption phenomena using radiopharmaceuticals. Radiopharmaceutical are medicines armed with a radionuclide enabling quantification and imaging using dedicated scanners. The sorption of nine different radiopharmaceuticals on 2- and 3-part syringes was investigated. These syringes were filled with the studied radiopharmaceutical solutions and stored immobile for 3 h. At different times ranging from 0 to 180 min, 10 µL were taken from the syringes and the radioactivity of these samples was determined by a gamma counter. 5 radiopharmaceuticals exhibited no significant sorption at any time point in both 2 and 3-parts syringes, but 4 radiopharmaceuticals exhibited sorption losses varying from 20 to 33% after 3 h contact with 3-part-syringes, but no sorption on 2-part syringes at any time point. [99mTc]Tc-tetrofosmine Single Photon Emission Computed Tomography/Computed Tomography imaging indicated clearly that the interactions were located on the rubber plunger of the 3-part-syringes. The specific nature of radiopharmaceuticals allowed their use as an innovative method to quantify and localize drug sorption phenomena.
Published: 2023
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2. Proton beam irradiation induces invisible modifications under the surface of painted parchment

Author: Müller, Katharina, Szikszai, Zita, Csepregi, Ákos, Huszánk, Róbert, Kertész, Zsófia, Reiche, Ina, Institut photonique d'analyse non-destructive européen des matériaux anciens (IPANEMA), Muséum national d'Histoire naturelle (MNHN)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture (MC), Institute for Nuclear Research (ATOMKI), University of Debrecen, Institut de Recherche de Chimie Paris (IRCP), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Ministère de la Culture (MC), New AGLAE - CNRS/Ministère de la Culture/ENSCP (new AGLAE), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: material science, physics, Multidisciplinary, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Physics, Science, Medicine, [SHS.MUSEO]Humanities and Social Sciences/Cultural heritage and museology, [CHIM.RADIO]Chemical Sciences/Radiochemistry, Article, Materials science
Abstract: International audience; Ion beam analysis plays an important role in cultural heritage (CH) studies as it offers a combination of simultaneous and complementary analytical techniques (PIXE/PIGE/RBS) and spatially resolved mapping functions. Despite being considered non-destructive, the potential risk of beam-induced modifications during analysis is increasingly discussed. This work focuses on the impact of proton beams on parchment, present in our CH in form of unique historical manuscripts. Parchment is one of the organic, protein-based CH materials believed to be the most susceptible to radiation-induced changes. Various modification patterns, observed on parchment cross-sections by optical and electron microscopy are reported: discoloration (yellowing), formation of cavities and denaturation of collagen fibers. Considerable modifications were detected up to 100 µm deep into the sample for beam fluences of 4 µC/cm 2 and higher. The presence of ultramarine paint on the parchment surface appears to increase the harmful effects of proton radiation. Based on our results, a maximum radiation dose of 0.5 µC/cm 2 can be considered as ‘safe boundary’ for 2.3 MeV PIXE analysis of parchment under the applied conditions.; L'analyse par faisceau d'ions joue un rôle important dans les études du patrimoine culturel (CH) car elle offre une combinaison de techniques analytiques simultanées et complémentaires (PIXE/PIGE/RBS) et de fonctions de cartographie résolues spatialement. Bien qu'il soit considéré comme non destructif, le risque potentiel de modifications induites par le faisceau lors de l'analyse est de plus en plus discuté. Ce travail porte sur l'impact des faisceaux de protons sur le parchemin, présent dans notre CH sous forme de manuscrits historiques uniques. Le parchemin est l'un des matériaux CH organiques à base de protéines que l'on pense être les plus sensibles aux changements induits par les rayonnements. Divers effets de modification, observés sur des coupes transversales de parchemin par microscopie optique et électronique sont rapportés : décoloration (jaunissement), formation de cavités et dénaturation des fibres de collagène. Des modifications considérables ont été détectées jusqu'à 100 µm de profondeur dans l'échantillon pour des doses de 4 µC/cm 2 et plus. La présence d'une couche picturale à base d'outremer sur la surface du parchemin semble augmenter les effets nocifs du rayonnement protonique. Sur la base de nos résultats, une dose de rayonnement maximale de 0,5 µC/cm 2 peut être considérée comme une « limite de sécurité » pour l'analyse PIXE de 2,3 MeV du parchemin dans les conditions appliquées.
Published: 2022
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3. Identification of a human estrogen receptor α tetrapeptidic fragment with dual antiproliferative and anti-nociceptive action

Author: Baptiste Jouffre, Alexandre Acramel, Mathilde Belnou, Maria Francesca Santolla, Marianna Talia, Rosamaria Lappano, Fariba Nemati, Didier Decaudin, Lucie Khemtemourian, Wang-Qing Liu, Marcello Maggiolini, Alain Eschalier, Christophe Mallet, Yves Jacquot, Neuro-Dol (Neuro-Dol), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA), Cibles Thérapeutiques et conception de médicaments (CiTCoM - UMR 8038), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Laboratoire des biomolécules (LBM UMR 7203), Chimie Moléculaire de Paris Centre (FR 2769), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Département de Chimie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Investigation Pré-clinique (LIP), Institut Curie [Paris], Chimie et Biologie des Membranes et des Nanoobjets (CBMN), and Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Multidisciplinary, [SDV]Life Sciences [q-bio]
Abstract: The synthetic peptide ERα17p (sequence: PLMIKRSKKNSLALSLT), which corresponds to the 295–311 region of the human estrogen receptor α (ERα), induces apoptosis in breast cancer cells. In mice and at low doses, it promotes not only the decrease of the size of xenografted triple-negative human breast tumors, but also anti-inflammatory and anti-nociceptive effects. Recently, we have shown that these effects were due to its interaction with the seven-transmembrane G protein-coupled estrogen receptor GPER. Following modeling studies, the C-terminus of this peptide (sequence: NSLALSLT) remains compacted at the entrance of the GPER ligand-binding pocket, whereas its N-terminus (sequence: PLMI) engulfs in the depth of the same pocket. Thus, we have hypothesized that the PLMI motif could support the pharmacological actions of ERα17p. Here, we show that the PLMI peptide is, indeed, responsible for the GPER-dependent antiproliferative and anti-nociceptive effects of ERα17p. By using different biophysical approaches, we demonstrate that the NSLALSLT part of ERα17p is responsible for aggregation. Overall, the tetrapeptide PLMI, which supports the action of the parent peptide ERα17p, should be considered as a hit for the synthesis of new GPER modulators with dual antiproliferative and anti-nociceptive actions. This study highlights also the interest to modulate GPER for the control of pain.
Published: 2023
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4. A proteome scale study reveals how plastic surfaces and agitation promote protein aggregation

Author: Marion Schvartz, Florent Saudrais, Stéphanie Devineau, Jean-Christophe Aude, Stéphane Chédin, Céline Henry, Aarón Millán-Oropeza, Thomas Perrault, Laura Pieri, Serge Pin, Yves Boulard, Guillaume Brotons, Jean-Philippe Renault, Laboratoire Interdisciplinaire sur l'Organisation Nanométrique et Supramoléculaire (LIONS), Nanosciences et Innovation pour les Matériaux, la Biomédecine et l'Energie (ex SIS2M) (NIMBE UMR 3685), Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Rayonnement Matière de Saclay (IRAMIS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut des Molécules et Matériaux du Mans (IMMM), Le Mans Université (UM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and ANR-18-IDEX-0001,Université de Paris,Université de Paris(2018)
Subjects: Multidisciplinary, proteome, [SDV]Life Sciences [q-bio], aggregation, [CHIM]Chemical Sciences
Abstract: Protein aggregation in biotherapeutics can reduce their activity and effectiveness. It may also promote immune reactions responsible for severe adverse effects. The impact of plastic materials on protein destabilization is not totally understood. Here, we propose to deconvolve the effects of material surface, air/liquid interface, and agitation to decipher their respective role in protein destabilization and aggregation. We analyzed the effect of polypropylene, TEFLON, glass and LOBIND surfaces on the stability of purified proteins (bovine serum albumin, hemoglobin and α-synuclein) and on a cell extract composed of 6000 soluble proteins during agitation (P = 0.1–1.2 W/kg). Proteomic analysis revealed that chaperonins, intrinsically disordered proteins and ribosomes were more sensitive to the combined effects of material surfaces and agitation while small metabolic oligomers could be protected in the same conditions. Protein loss observations coupled to Raman microscopy, dynamic light scattering and proteomic allowed us to propose a mechanistic model of protein destabilization by plastics. Our results suggest that protein loss is not primarily due to the nucleation of small aggregates in solution, but to the destabilization of proteins exposed to material surfaces and their subsequent aggregation at the sheared air/liquid interface, an effect that cannot be prevented by using LOBIND tubes. A guidance can be established on how to minimize these adverse effects. Remove one of the components of this combined stress - material, air (even partially), or agitation - and proteins will be preserved.
Published: 2023
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5. Novel markers to early detect degradation on cellulose nitrate-based heritage at the submicrometer level using synchrotron UV–VIS multispectral luminescence

Author: Maria Elvira Callapez, Maria João Melo, Artur Neves, Ana M. Ramos, Mathieu Thoury, Robert Friedel, Matthieu Réfrégiers, LAQV@REQUIMTE, DCR - Departamento de Conservação e Restauro, DQ - Departamento de Química, Refregiers, Matthieu, Laboratorio Associado para a Quimica Verde (LAQV), Requimte, Universidade do Porto = University of Porto-Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Centro Interuniversitário de História das Ciências e da Tecnologia [Caparica] (CIUHCT), University of Maryland [Baltimore], Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Institut photonique d'analyse non-destructive européen des matériaux anciens (IPANEMA), Muséum national d'Histoire naturelle (MNHN)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture (MC), This work was supported by the European Union’s Horizon 2020 Research and Innovation Programme (H2020-NMBP‐35‐2017), Grant agreement no 76081, Project NEMOSINE, 'Innovative packaging solutions for storage and conservation of 20th century cultural heritage of artefacts based on cellulose derivatives, and Fundação para a Ciência e a Tecnologia, Ministério da Ciência Tecnologia e Ensino Superior (FCT/MCTES), Portugal, through doctoral programme CORES-PD/00253/2012, PhD Grant PD/BD/136678/2018 (Artur Neves) and Associate Laboratory for Green Chemistry- LAQV financed by national funds (UIDB/ 50006/2020 and UIDP/50006/2020)., Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), and Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)
Subjects: Materials science, [CHIM.ANAL] Chemical Sciences/Analytical chemistry, Photochemistry, Science, Multispectral image, Nanotechnology, 02 engineering and technology, 01 natural sciences, Article, law.invention, Celluloid, chemistry.chemical_compound, Ultraviolet visible spectroscopy, Nitrate, SDG 3 - Good Health and Well-being, law, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, History of chemistry, Cellulose, General, Multidisciplinary, 010401 analytical chemistry, [SHS.ART]Humanities and Social Sciences/Art and art history, 021001 nanoscience & nanotechnology, Synchrotron, 0104 chemical sciences, Optics and photonics, Physical chemistry, chemistry, visual_art, visual_art.visual_art_medium, Degradation (geology), Medicine, [SHS.ART] Humanities and Social Sciences/Art and art history, 0210 nano-technology, Luminescence
Abstract: 20200316 H2020-NMBP‐35‐2017 CORES-PD/00253/2012 PD/BD/136678/2018 UIDB/ 50006/2020 UIDP/50006/2020 Cellulose nitrate (CN) is an intrinsically unstable material that puts at risk the preservation of a great variety of objects in heritage collections, also posing threats to human health. For this reason, a detailed investigation of its degradation mechanisms is necessary to develop sustainable conservation strategies. To investigate novel probes of degradation, we implemented deep UV photoluminescence micro spectral-imaging, for the first time, to characterize a corpus of historical systems composed of cellulose nitrate. The analysis of cinematographic films and everyday objects dated from the nineteenth c./early twentieth c. (Perlov's collection), as well as of photo-aged CN and celluloid references allowed the identification of novel markers that correlate with different stages of CN degradation in artworks, providing insight into the role played by plasticizers, fillers, and other additives in stability. By comparison with photoaged references of CN and celluloid (70% CN and 30% camphor), it was possible to correlate camphor concentration with a higher rate of degradation of the cinematographic films. Furthermore, the present study investigates, at the sub-microscale, materials heterogeneity that correlates to the artworks' history, associating the different emission profiles of zinc oxide to specific color formulations used in the late nineteenth and early twentieth centuries. publishersversion published
Published: 2021
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6. Combining OSMAC, metabolomic and genomic methods for the production and annotation of halogenated azaphilones and ilicicolins in termite symbiotic fungi

Author: Téo Hebra, Nicolas Pollet, David Touboul, Véronique Eparvier, Institut de Chimie des Substances Naturelles (ICSN), Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Evolution, génomes, comportement et écologie (EGCE), Institut de Recherche pour le Développement (IRD)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire de chimie moléculaire (LCM), and École polytechnique (X)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Multidisciplinary, Tandem Mass Spectrometry, Polyketides, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Flavin-Adenine Dinucleotide, Animals, Benzopyrans, Genomics, Isoptera, Pigments, Biological, [CHIM.CHEM]Chemical Sciences/Cheminformatics, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
Abstract: International audience; AbstractWe gathered a collection of termite mutualistic strains from French Guiana to explore the metabolites of symbiotic microorganisms. Molecular networks reconstructed from a metabolomic analysis using LC–ESI–MS/MS methodology led us to identify two families of chlorinated polyketides, i.e., azaphilones from Penicillium sclerotiorum and ilicicolins from Neonectria discophora. To define the biosynthetic pathways related to these two types of scaffolds, we used a whole genome sequencing approach followed by hybrid assembly from short and long reads. We found two biosynthetic gene clusters, including two FAD-dependent halogenases. To exploit the enzymatic promiscuity of the two identified FAD halogenases, we sought to biosynthesize novel halogenated metabolites. An OSMAC strategy was used and resulted in the production of brominated analogs of ilicicolins and azaphilones as well as iodinated analogs of azaphilones.
Published: 2022
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7. Metallomic profile in non-cirrhotic hepatocellular carcinoma supports a phenomenon of metal metabolism adaptation in tumor cells

Author: Luis Cano, Stéphane Bertani, Marie-Laure Island, Juan Pablo Cerapio, Eloy Ruiz, Pascal Pineau, Valérie Monbet, Karim Boudjema, Luis Taxa, Sandro Casavilca-Zambrano, Martine Ropert, Bruno Turlin, Olivier Loréal, Nutrition, Métabolismes et Cancer (NuMeCan), Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Pharmacochimie et Biologie pour le Développement (PHARMA-DEV), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT), Institut Universitaire du Cancer de Toulouse - Oncopole (IUCT Oncopole - UMR 1037), Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM), Instituto Nacional de Enfermedades Neoplasicas [Lima, Pérou] (INEN), Organisation Nucléaire et Oncogenèse / Nuclear Organization and Oncogenesis, Institut Pasteur [Paris] (IP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Cité (UPCité), Institut de Recherche Mathématique de Rennes (IRMAR), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École normale supérieure - Rennes (ENS Rennes)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centre Hospitalier Universitaire [Rennes], This study was funded by the French National Alliance for Life Sciences and Health (ENV201408), LC was a recipient of a doctoral fellowship from French National Research Institute for Sustainable Development (IRD) (EMHE-ARTS-2016-878573B), JPC was a recipient of a doctoral fellowship from the Peruvian National Coun-cil for Science and Technology (212-2015-FONDECYT), SB has received funding from the European Union’s Horizon 2020 Framework program under the Marie Skłodowska-Curie Actions 823935 and from the Alliance pour les Sciences de la Vie et de la Santé (AVIESAN), ITMO Cancer ENV201408, and LC, ER, and SCZ have received funding from the World Bank Group and FONDECYT-CONCYTEC 016-2018-FONDECYT/BM., European Project: 823935,H2020-MSCA-RISE-2018,COCLICAN(2018), Institut de Recherche pour le Développement (IRD)-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre Hospitalier Universitaire de Toulouse (CHU Toulouse)-Institut National de la Santé et de la Recherche Médicale (INSERM), Bertani, Stéphane, Collaborative Consortium for the early detection of Liver Cancer - COCLICAN - - H2020-MSCA-RISE-20182018-11-01 - 2022-10-31 - 823935 - VALID, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Institut de Recherche pour le Développement (IRD), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-CHU Toulouse [Toulouse]-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris]-Institut National de la Santé et de la Recherche Médicale (INSERM), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Université de Rennes 2 (UR2), Université de Rennes (UNIV-RENNES)-École normale supérieure - Rennes (ENS Rennes)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)
Subjects: Adult, Male, Carcinoma, Hepatocellular, Science, [SDV.CAN]Life Sciences [q-bio]/Cancer, Article, Young Adult, [SDV.CAN] Life Sciences [q-bio]/Cancer, parasitic diseases, Humans, neoplasms, Aged, Retrospective Studies, Liver Neoplasms, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, Middle Aged, Cancer metabolism, [SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, digestive system diseases, 3. Good health, Liver, Metals, Medicine, Female, Liver cancer
Abstract: Our group has previously described a particular form of Hepatocellular carcinoma (HCC) developed in non-cirrhotic liver (HCC-NC) developed in Peruvian patients Our aim is to analyze the HCC-NC from clinical-biological findings in two different cohorts (Peruvian and French) and the link with metallomic profile. Clinical, histopathological and also tumoral (T) and non-tumoral liver (NTL) samples of 38 Peruvian and 45 French patients were studied. Twelve metals were assayed using ICP/MS: Mn, Fe, Cu, Co, Zn, As, Se, Rb, Mo, Cd, Pb, and Sn. Possible associations between metals and survival were also evaluated. Overall, results show clearly differences between both cohorts. Mean age were 41 ± 20 and 68 ± 9 years-old for Peruvians and French, respectively. 80% of Peruvian patients were positive for the HBsAg, versus 9% in French patients. Metals concentrations were higher in the NTL of Peruvians (p
Published: 2021
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8. Dissection of the anti-Candida albicans mannan immune response using synthetic oligomannosides reveals unique properties of β-1,2 mannotriose protective epitopes

Author: Sébastien Damiens, Faustine Dubar, Samir Jawhara, Boualem Sendid, Mayeul Collot, Karine Lecointe, Jean-Maurice Mallet, Anne-Sophie Drucbert, Daniel Poulain, Chantal Fradin, Pierre-Marie Danzé, Jean-Pierre Vilcot, Jérôme de Ruyck, Frédéric Grenouillet, Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS), Laboratoire des biomolécules (LBM UMR 7203), Chimie Moléculaire de Paris Centre (FR 2769), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Département de Chimie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Facteurs de Risque et Déterminants Moléculaires des Maladies liées au Vieillissement - U 1167 (RID-AGE), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), Optoélectronique - IEMN (OPTO - IEMN), INSA Institut National des Sciences Appliquées Hauts-de-France (INSA Hauts-De-France), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), Centre Hospitalier Régional Universitaire de Besançon (CHRU Besançon), We thank the European Union for funding through the INTERREG V France—Wallonie—Vlaanderen program—SmartBioControl-BIOSENS project and the Programme Hospitalier de Recherche Clinique, CandiGene, 2011–1918., Unité de Glycobiologie Structurale et Fonctionnelle (UGSF), Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Chimie Moléculaire de Paris Centre (FR 2769), Institut de Chimie du CNRS (INC)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire des biomolécules [LBM UMR 7203], Institut de Pathologie [CHU Lille], Laboratoire de Bioimagerie et Pathologies [LBP], Médicaments et biomatériaux à libération contrôlée: mécanismes et optimisation - Advanced Drug Delivery Systems - U 1008 [MBLC - ADDS], Lille Inflammation Research International Center - U 995 [LIRIC], Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 [IEMN], Laboratoire Chrono-environnement (UMR 6249) [LCE], Unité de Glycobiologie Structurale et Fonctionnelle - UMR 8576 [UGSF], and Collot, Mayeul
Subjects: Antigens, Fungal, medicine.drug_class, [SDV]Life Sciences [q-bio], Science, Immunology, Oligosaccharides, Monoclonal antibody, Biochemistry, Microbiology, Article, Epitope, Mannans, 03 medical and health sciences, Antigen, Antibody Specificity, Candida albicans, medicine, 030304 developmental biology, Mannan, 0303 health sciences, Multidisciplinary, biology, 030306 microbiology, Chemistry, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Candidiasis, Antibodies, Monoclonal, Surface Plasmon Resonance, [CHIM.ORGA] Chemical Sciences/Organic chemistry, biology.organism_classification, Chemical biology, Corpus albicans, 3. Good health, [SDV] Life Sciences [q-bio], Polyclonal antibodies, biology.protein, Medicine, Antibody, Trisaccharides, Epitope Mapping
Abstract: Candida albicans mannan consists of a large repertoire of oligomannosides with different types of mannose linkages and chain lengths, which act as individual epitopes with more or less overlapping antibody specificities. Although anti-C. albicans mannan antibody levels are monitored for diagnostic purposes nothing is known about the qualitative distribution of these antibodies in terms of epitope specificity. We addressed this question using a bank of previously synthesized biotin sulfone tagged oligomannosides (BSTOs) of α and β anomery complemented with a synthetic β-mannotriose described as a protective epitope. The reactivity of these BSTOs was analyzed with IgM isotype monoclonal antibodies (MAbs) of known specificity, polyclonal sera from patients colonized or infected with C. albicans, and mannose binding lectin (MBL). Surface plasmon resonance (SPR) and multiple analyte profiling (MAP) were used. Both methods confirmed the usual reactivity of MAbs against either α or β linkages, excepted for MAb B6.1 (protective epitope) reacting with β-Man whereas the corresponding BSTO reacted with anti-α-Man. These results were confirmed in western blots with native C. albicans antigens. Using patients’ sera in MAP, a significant correlation was observed between the detection of anti-mannan antibodies recognizing β- and α-Man epitopes and detection of antibodies against β-linked mannotriose suggesting that this epitope also reacts with human polyclonal antibodies of both specificities. By contrast, the reactivity of human sera with other α- and β-linked BSTOs clearly differed according to their colonized or infected status. In these cases, the establishment of an α/β ratio was extremely discriminant. Finally SPR with MBL, an important lectin of innate immunity to C. albicans, classically known to interact with α-mannose, also interacted in an unexpected way with the protective epitope. These cumulative data suggest that structure/activity investigations of the finely tuned C. albicans anti-mannose immune response are worthwhile to increase our basic knowledge and for translation in medicine.
Published: 2021
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9. Influence of intraoperative vasopressor use on indocyanine green fluorescence angiography: first evaluation in an experimental model

Author: Eric Noll, René R. W. J. van der Hulst, Mahdi Al-Taher, Tim Pruimboom, Alexandre Hostettler, Rutger M. Schols, Michele Diana, Nariaki Okamoto, Laurents P. S. Stassen, Michael Kugler, Sophie Diemunsch, Nicole D. Bouvy, Jacques Marescaux, IRCAD, Maastricht University Medical Centre (MUMC), Maastricht University [Maastricht], CHU Strasbourg, Laboratoire des sciences de l'ingénieur, de l'informatique et de l'imagerie (ICube), École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Université de Strasbourg (UNISTRA)-Institut National des Sciences Appliquées - Strasbourg (INSA Strasbourg), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Les Hôpitaux Universitaires de Strasbourg (HUS)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), univOAK, Archive ouverte, MUMC+: MA Heelkunde (9), Surgery, RS: NUTRIM - R2 - Liver and digestive health, Plastische Chirurgie (PLC), MUMC+: MA AIOS Plastische Chirurgie (9), MUMC+: MA AIOS Heelkunde (9), MUMC+: MA Plastische Chirurgie (3), and MUMC+: MA Plastische Chirurgie (9)
Subjects: Indocyanine Green, ANASTOMOTIC LEAKAGE, Swine, [SDV.IB.IMA]Life Sciences [q-bio]/Bioengineering/Imaging, Science, Article, Norepinephrine (medication), 03 medical and health sciences, chemistry.chemical_compound, Intraoperative Period, Norepinephrine, 0302 clinical medicine, COLORECTAL SURGERY, PERFUSION, medicine, Animals, Vasoconstrictor Agents, Gastrointestinal models, Fluorescein Angiography, Infusions, Intravenous, Laparotomy, Multidisciplinary, medicine.diagnostic_test, Experimental model, business.industry, Imaging and sensing, EPINEPHRINE, PHENYLEPHRINE, Intestines, Fluorescence intensity, Disease Models, Animal, Epinephrine, [SDV.IB.IMA] Life Sciences [q-bio]/Bioengineering/Imaging, chemistry, 030220 oncology & carcinogenesis, Angiography, Injections, Intravenous, Medicine, 030211 gastroenterology & hepatology, Female, ENHANCED REALITY, business, Nuclear medicine, Perfusion, Indocyanine green, medicine.drug, Indocyanine green fluorescence
Abstract: Intraoperative indocyanine green (ICG) fluorescence angiography has gained popularity and acceptance in many surgical fields for the real-time assessment of tissue perfusion. Although vasopressors have the potential to preclude an accurate assessment of tissue perfusion, there is a lack of literature with regards to its effect on ICG fluorescence angiography. An experimental porcine model was used to expose the small bowel for quantitative tissue perfusion assessment. Three increasing doses of norepinephrine infusion (0.1, 0.5, and 1.0 µg/kg/min) were administered intravenously over a 25-min interval. Time-to-peak fluorescence intensity (TTP) was the primary outcome. Secondary outcomes included absolute fluorescence intensity and local capillary lactate (LCL) levels. Five large pigs (mean weight: 40.3 ± 4.24 kg) were included. There was no significant difference in mean TTP (in seconds) at baseline (4.23) as compared to the second (3.90), third (4.41), fourth (4.60), and fifth ICG assessment (5.99). As a result of ICG accumulation, the mean and the maximum absolute fluorescence intensity were significantly different as compared to the baseline assessment. There was no significant difference in LCL levels (in mmol/L) at baseline (0.74) as compared to the second (0.82), third (0.64), fourth (0.60), and fifth assessment (0.62). Increasing doses of norepinephrine infusion have no significant influence on bowel perfusion using ICG fluorescence angiography.
Published: 2021
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10. Non-oxide precipitates in additively manufactured austenitic stainless steel

Author: Upadhyay, Manas Vijay, Slama, Meriem Ben Haj, Gaudez, Steve, Mohanan, Nikhil, Yedra, Lluis, Hallais, Simon, Héripré, Eva, Tanguy, Alexandre, Laboratoire de mécanique des solides (LMS), École polytechnique (X)-MINES ParisTech - École nationale supérieure des mines de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de mécanique des sols, structures et matériaux (MSSMat), CentraleSupélec-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institut Jean Lamour (IJL), Université de Lorraine (UL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Structures, Propriétés et Modélisation des solides (SPMS), Institut de Chimie du CNRS (INC)-CentraleSupélec-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), This work has benefited from the support of (i) the Fédération Francielliene de Mécanique (F2M) through the Coup de Pouce 2019 grant and (ii) the European Research Council (ERC) through the European Union’s Horizon 2020 research and innovation programme (Grant agreement No. 946959)., ERC Starting Grant No. 946959, European Project: GAMMA, École polytechnique (X)-Mines Paris - PSL (École nationale supérieure des mines de Paris), and Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)
Subjects: directed energy deposition, precipitate, Three-dimensional printing, Science, Metals and alloys, [CHIM.MATE]Chemical Sciences/Material chemistry, Sulfides, gas-atomization, Article, laser metal deposition, [SPI.MAT]Engineering Sciences [physics]/Materials, Sulfurs, [SPI]Engineering Sciences [physics], Engineering, Microscòpia electrònica, transmission electron microscopy, Electron microscopy, Medicine, Impressió 3D
Abstract: International audience; Precipitates in an austenitic stainless steel fabricated via any Additive Manufacturing (AM), or 3D printing, technique have been widely reported to be only Mn-Si-rich oxides. However, via Transmission Electron Microscopy (TEM) studies on a 316L stainless steel, we show that non-oxide precipitates (intermetallics, sulfides, phosphides and carbides) can also form when the steel is fabricated via Laser Metal Deposition (LMD)-a directed energy deposition-type AM technique. An investigation into their origin is conducted with support from precipitation kinetics and finite element heat transfer simulations. It reveals that non-oxide precipitates form during solidification/cooling at temperatures ≥ 0.75Tm (melting point) and temperature rates ≤ 10 5 K/s, which is the upper end of the maximum rates encountered during LMD but lower than those encountered during Selective Laser Melting (SLM)-a powder-bed type AM technique. Consequently, non-oxide precipitates should form during LMD, as reported in this work, but not during SLM, in consistency with existing literature.
Published: 2021
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11. New insights into structure and function of bis-phosphinic acid derivatives and implications for CFTR modulation

Author: Guillaume Prestat, Jean-Paul Mornon, Danielle Tondelier, Sara Bitam, Nathalie Servel, Agathe Lepissier, Mélanie Faria Da Cunha, Ahmad Elbahnsi, Isabelle Sermet-Gaudelus, Alexandra Pastor, Isabelle Callebaut, Christelle Moquereau, Geordie Creste, A. Hatton, Iwona Pranke, Farouk Berhal, Nesrine Baatalah, Aleksander Edelman, Brice Hoffmann, Christine Gravier-Pelletier, Alexandre Hinzpeter, Benoit Chevalier, Gestionnaire, HAL Sorbonne Université 5, Institut Necker Enfants-Malades (INEM - UM 111 (UMR 8253 / U1151)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut de minéralogie, de physique des matériaux et de cosmochimie (IMPMC), Muséum national d'Histoire naturelle (MNHN)-Institut de recherche pour le développement [IRD] : UR206-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques (LCBPT - UMR 8601), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Université Paris Cité, CNRS, Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques, F-75006 Paris, France, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)
Subjects: 0301 basic medicine, Cell biology, Cystic Fibrosis, [SDV]Life Sciences [q-bio], Science, Cystic Fibrosis Transmembrane Conductance Regulator, Bronchi, Diseases, medicine.disease_cause, 01 natural sciences, Article, 03 medical and health sciences, Medical research, 0103 physical sciences, Side chain, medicine, Humans, Mode of action, Author Correction, Cells, Cultured, Mutation, Multidisciplinary, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, 010304 chemical physics, biology, Molecular medicine, Chemistry, Mutagenesis, Homozygote, Phosphinic Acids, Cystic fibrosis transmembrane conductance regulator, Molecular Docking Simulation, 030104 developmental biology, Biophysics, biology.protein, Medicine, Nasal Cavity, Function (biology), Intracellular, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Binding domain
Abstract: C407 is a compound that corrects the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein carrying the p.Phe508del (F508del) mutation. We investigated the corrector effect of c407 and its derivatives on F508del-CFTR protein. Molecular docking and dynamics simulations combined with site-directed mutagenesis suggested that c407 stabilizes the F508del-Nucleotide Binding Domain 1 (NBD1) during the co-translational folding process by occupying the position of the p.Phe1068 side chain located at the fourth intracellular loop (ICL4). After CFTR domains assembly, c407 occupies the position of the missing p.Phe508 side chain. C407 alone or in combination with the F508del-CFTR corrector VX-809, increased CFTR activity in cell lines but not in primary respiratory cells carrying the F508del mutation. A structure-based approach resulted in the synthesis of an extended c407 analog G1, designed to improve the interaction with ICL4. G1 significantly increased CFTR activity and response to VX-809 in primary nasal cells of F508del homozygous patients. Our data demonstrate that in-silico optimized c407 derivative G1 acts by a mechanism different from the reference VX-809 corrector and provide insights into its possible molecular mode of action. These results pave the way for novel strategies aiming to optimize the flawed ICL4–NBD1 interface.
Published: 2021
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12. Replication of Equine arteritis virus is efficiently suppressed by purine and pyrimidine biosynthesis inhibitors

Author: Patrick Dallemagne, Pierre-Olivier Vidalain, Aymeric Hans, Anthony Madeline, D. Gaudaire, José Carlos Valle-Casuso, Lydie Martin-Faivre, Stéphane Pronost, Hélène Munier-Lehmann, Stéphan Zientara, Physiopathologie et épidémiologie des maladies équines (PhEED), Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Centre d'Etudes et de Recherche sur le Médicament de Normandie (CERMN), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), LABÉO, Pôle d’analyses et de recherche de Normandie (LABÉO), Biologie, génétique et thérapies ostéoarticulaires et respiratoires (BIOTARGEN), Chimie et Biocatalyse, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Virologie UMR1161 (VIRO), École nationale vétérinaire - Alfort (ENVA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie et de Biochimie Pharmacologiques et Toxicologiques (LCBPT - UMR 8601), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Fonds Eperon, AnsesFonds EperonRegion Normandie, IFCE (Institut Francais du Cheval et de l'Equitation), Région Normandie, ANSES, Laboratoire de Santé Animale - site de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Université Paris-Est (UPE), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), This work was supported by Anses, IFCE (Institut Français du Cheval et de l’Equitation), Fonds Eperon and Région Normandie., AnsesIFCE (Institut Francais du Cheval et de l'Equitation)Fonds EperonRegion Normandie, Bodescot, Myriam, École nationale vétérinaire - Alfort (ENVA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Dihydroorotate Dehydrogenase, lcsh:Medicine, Virus Replication, chemistry.chemical_compound, Cytopathogenic Effect, Viral, Coronaviridae, lcsh:Science, [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology, 0303 health sciences, education.field_of_study, Multidisciplinary, Arterivirus Infections, Drug discovery, 3. Good health, [SDV.SP.PHARMA] Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, Pyrimidine metabolism, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Oxidoreductases Acting on CH-CH Group Donors, 030106 microbiology, Population, Biology, Antiviral Agents, Microbiology, Article, Cell Line, 03 medical and health sciences, Equartevirus, Ribavirin, Animals, Horses, education, 030304 developmental biology, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, 030306 microbiology, lcsh:R, [SDV.BA.MVSA] Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, RNA, RNA virus, biology.organism_classification, Virology, 030104 developmental biology, Pyrimidines, chemistry, Viral replication, Purines, Dihydroorotate dehydrogenase, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, Horse Diseases, lcsh:Q
Abstract: RNA viruses are responsible for a large variety of animal infections. Equine Arteritis Virus (EAV) is a positive single-stranded RNA virus member of the family Arteriviridae from the order Nidovirales like the Coronaviridae. EAV causes respiratory and reproductive diseases in equids. Although two vaccines are available, the vaccination coverage of the equine population is largely insufficient to prevent new EAV outbreaks around the world. In this study, we present a high-throughput in vitro assay suitable for testing candidate antiviral molecules on equine dermal cells infected by EAV. Using this assay, we identified three molecules that impair EAV infection in equine cells: the broad-spectrum antiviral and nucleoside analog ribavirin, and two compounds previously described as inhibitors of dihydroorotate dehydrogenase (DHODH), the fourth enzyme of the pyrimidine biosynthesis pathway. These molecules effectively suppressed cytopathic effects associated to EAV infection, and strongly inhibited viral replication and production of infectious particles. Since ribavirin is already approved in human and small animal, and that several DHODH inhibitors are in advanced clinical trials, our results open new perspectives for the management of EAV outbreaks.
Published: 2020
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13. Hepatic stellate cell hypertrophy is associated with metabolic liver fibrosis

Author: Pascal Roux, Virginie Escriou, Judith Aron-Wisnewsky, Nour El Houda Djerir, Christine Charrueau, Isabelle Brocheriou, Céline Hoffmann, Anne Danckaert, Karine Clément, Fabienne Foufelle, Anne-Marie Lachagès, Frédéric Charlotte, Vlad Ratziu, Julien Fernandes, Bernard Hainque, Pascal Bigey, Dominique Bonnefont-Rousselot, Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS - UM 4 (UMR 8258 / U1022)), Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), BioImagerie Photonique – Photonic BioImaging (UTechS PBI), Institut Pasteur [Paris], Service d'Anatomie et cytologie pathologiques [CHU Pitié-Salpêtrière] (ACP), CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service de Nutrition [CHU Pitié-Salpétrière], Institut E3M [CHU Pitié-Salpêtrière], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP], Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Institute of cardiometabolism and nutrition (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service de Biochimie Métabolique [CHU Pitié-Salpêtrière], Université Paris sciences et lettres (PSL), This work was supported by the Agence Nationale de la Recherche (ANR, ANR Fibrother ANR-18-CE18-0005-01 to C.H.). The UtechS PBI (A.D., J.F., and P.R.) is part of the France BioImaging infrastructure supported by the French National Research Agency (ANR-10-INSB-04-01, 'Investments for the future'). Funding for patient’s recruitment (K.C.) was obtained by the Clinical Research Contrat (CRC-Fibrota)., ANR-18-CE18-0005,FIBROTHER,Nanovecteurs polyvalents pour la thérapie de la fibrose hépatique(2018), ANR-10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL (ENSCP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Technologie et Service BioImagerie Photonique – Photonic BioImaging (UTechS PBI), Centre de Ressources et de Recherche Technologique - Center for Technological Resources and Research (C2RT), Institut Pasteur [Paris]-Institut Pasteur [Paris], Service d’anatomie et cytologie pathologiques [CHU Pitié-Salpêtrière], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Pitié-Salpêtrière [APHP], Service de nutrition [CHU Pitié-Salpétrière], École pratique des hautes études (EPHE)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-CHU Pitié-Salpêtrière [APHP]-Sorbonne Université (SU), Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL (ENSCP), ANR-18-CE18-0005,FIBROTHER,MULTIPLEXED NANOVECTORS FOR METABOLIC LIVER FIBROSIS THERAPY(2018), ANR-10-INBS-04-01/10-INBS-0004,France-BioImaging,Développment d'une infrastructure française distribuée coordonnée(2010), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur [Paris] (IP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Nutrition et obésités: approches systémiques (UMR-S 1269) (Nutriomics), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), École Pratique des Hautes Études (EPHE), Unité de Recherche sur les Maladies Cardiovasculaires, du Métabolisme et de la Nutrition = Research Unit on Cardiovascular and Metabolic Diseases (ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Institut de Cardiométabolisme et Nutrition = Institute of Cardiometabolism and Nutrition [CHU Pitié Salpêtrière] (IHU ICAN), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-CHU Pitié-Salpêtrière [AP-HP], Service d'Anatomie et cytologie pathologiques = Service de Pathologie [CHU Pitié-Salpêtrière] (ACP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Bodescot, Myriam, APPEL À PROJETS GÉNÉRIQUE 2018 - Nanovecteurs polyvalents pour la thérapie de la fibrose hépatique - - FIBROTHER2018 - ANR-18-CE18-0005 - AAPG2018 - VALID, Développment d'une infrastructure française distribuée coordonnée - - France-BioImaging2010 - ANR-10-INBS-0004 - INBS - VALID, and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Pitié-Salpêtrière [AP-HP]
Subjects: 0301 basic medicine, Liver Cirrhosis, Male, Pathology, medicine.medical_specialty, Liver fibrosis, lcsh:Medicine, Context (language use), [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Chronic liver disease, Article, Muscle hypertrophy, 03 medical and health sciences, Liver disease, Mice, 0302 clinical medicine, Fibrosis, Hepatic stellate cells, medicine, Animals, Humans, lcsh:Science, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Non-alcoholic steatohepatitis, Multidisciplinary, business.industry, Carbon Tetrachloride Poisoning, lcsh:R, [SDV.MHEP.HEG]Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, medicine.disease, Dietary Fats, [SDV.MHEP.HEG] Life Sciences [q-bio]/Human health and pathology/Hépatology and Gastroenterology, 3. Good health, 030104 developmental biology, 030220 oncology & carcinogenesis, Hepatic stellate cell, lcsh:Q, Steatohepatitis, business, Hepatic fibrosis
Abstract: Hepatic fibrosis is a major consequence of chronic liver disease such as non-alcoholic steatohepatitis which is undergoing a dramatic evolution given the obesity progression worldwide, and has no treatment to date. Hepatic stellate cells (HSCs) play a key role in the fibrosis process, because in chronic liver damage, they transdifferentiate from a “quiescent” to an “activated” phenotype responsible for most the collagen deposition in liver tissue. Here, using a diet-induced liver fibrosis murine model (choline-deficient amino acid-defined, high fat diet), we characterized a specific population of HSCs organized as clusters presenting simultaneously hypertrophy of retinoid droplets, quiescent and activated HSC markers. We showed that hypertrophied HSCs co-localized with fibrosis areas in space and time. Importantly, we reported the existence of this phenotype and its association with collagen deposition in three other mouse fibrosis models, including CCl4-induced fibrosis model. Moreover, we have also shown its relevance in human liver fibrosis associated with different etiologies (obesity, non-alcoholic steatohepatitis, viral hepatitis C and alcoholism). In particular, we have demonstrated a significant positive correlation between the stage of liver fibrosis and HSC hypertrophy in a cohort of obese patients with hepatic fibrosis. These results lead us to conclude that hypertrophied HSCs are closely associated with hepatic fibrosis in a metabolic disease context and may represent a new marker of metabolic liver disease progression.
Published: 2020
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14. Structural features of the interaction of MapZ with FtsZ and membranes in Streptococcus pneumoniae

Author: Denis Martinez, Jean-Pierre Simorre, Catherine M. Bougault, Birgit Habenstein, Jean-Pierre Lavergne, Isabel Ayala, Daphna Fenel, Marine Restelli, Christophe Grangeasse, Tomas Hosek, Cécile Morlot, Institut de biologie structurale (IBS - UMR 5075), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Grenoble Alpes (UGA), Microbiologie moléculaire et biochimie structurale / Molecular Microbiology and Structural Biochemistry (MMSB), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Chimie et Biologie des Membranes et des Nanoobjets (CBMN), Université de Bordeaux (UB)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), iR-RMN-THC Fr3050 CNRS, ISBG, UMS 3518 CNRS-CEA-UGA-EMBL, Plateforme RMN, Microscopie électronique, ANR-15-CE32-0001,Map-CellDiv,MapZ: caractérisation d'un nouveau mécanisme de régulation de la division cellulaire bactérienne(2015), ANR-10-INBS-0005,FRISBI,Infrastructure Française pour la Biologie Structurale Intégrée(2010), ANR-17-EURE-0003,CBH-EUR-GS,CBH-EUR-GS(2017), Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Sciences et Technologies - Bordeaux 1-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Centre National de la Recherche Scientifique (CNRS), ANR-10-INBS-0005-02,FRISBI,Infrastructure Française pour la Biologie Structurale Intégrée(2010), and École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Cell division, 030106 microbiology, lcsh:Medicine, Article, Cell membrane, 03 medical and health sciences, NMR spectroscopy, Bacterial Proteins, Bacterial development, Extracellular, medicine, Electron microscopy, FtsZ, lcsh:Science, Bacterial structural biology, Multidisciplinary, biology, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Chemistry, Cell Membrane, lcsh:R, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Transmembrane domain, Cytoskeletal Proteins, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biomolecules [q-bio.BM], 030104 developmental biology, Membrane, medicine.anatomical_structure, Streptococcus pneumoniae, Structural biology, Cytoplasm, Biophysics, biology.protein, lcsh:Q, Pathogens
Abstract: MapZ localizes at midcell and acts as a molecular beacon for the positioning of the cell division machinery in the bacterium Streptococcus pneumoniae. MapZ contains a single transmembrane helix that separates the C-terminal extracellular domain from the N-terminal cytoplasmic domain. Only the structure and function of the extracellular domain is known. Here, we demonstrate that large parts of the cytoplasmic domain is intrinsically disordered and that there are two regions (from residues 45 to 68 and 79 to 95) with a tendency to fold into amphipathic helices. We further reveal that these regions interact with the surface of liposomes that mimic the Streptococcus pneumoniae cell membrane. The highly conserved and unfolded N-terminal region (from residues 17 to 43) specifically interacts with FtsZ independently of FtsZ polymerization state. Moreover, we show that MapZ phosphorylation at positions Thr67 and Thr68 does not impact the interaction with FtsZ or liposomes. Altogether, we propose a model in which the MapZ-mediated recruitment of FtsZ to mid-cell is modulated through competition of MapZ binding to the cell membrane. The molecular interplay between the components of this tripartite complex could represent a key step toward the complete assembly of the divisome.
Published: 2020
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15. A new versatile MR-guided high-intensity focused ultrasound (HIFU) device for the treatment of musculoskeletal tumors

Author: Paolo Cabras, Pierre Auloge, Fabrice Bing, Pramod Prabhakar Rao, Stéphanie Hoarau, Erik Dumont, Alexandre Durand, Benjamin Maurin, Benoit Wach, Loïc Cuvillon, Elodie Breton, Afshin Gangi, Jonathan Vappou, Laboratoire des sciences de l'ingénieur, de l'informatique et de l'imagerie (ICube), École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Université de Strasbourg (UNISTRA)-Institut National des Sciences Appliquées - Strasbourg (INSA Strasbourg), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Les Hôpitaux Universitaires de Strasbourg (HUS)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, and Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Multidisciplinary, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, High-Intensity Focused Ultrasound Ablation, Humans, Pain, Thermometry, Magnetic Resonance Imaging, Neoplasms, Connective and Soft Tissue
Abstract: Magnetic Resonance (MR) Imaging-guided High Intensity focused Ultrasound (MRgHIFU) is a non-invasive, non-ionizing thermal ablation therapy that is particularly interesting for the palliative or curative treatment of musculoskeletal tumors. We introduce a new modular MRgHIFU device that allows the ultrasound transducer to be positioned precisely and interactively over the body part to be treated. A flexible, MR-compatible supporting structure allows free positioning of the transducer under MRI/optical fusion imaging guidance. The same structure can be rigidified using pneumatic depression, holding the transducer rigidly in place. Targeting accuracy was first evaluated in vitro. The average targeting error of the complete process was found to be equal to 5.4 ± 2.2 mm in terms of focus position, and 4.7° ± 2° in terms of transducer orientation. First-in-man feasibility is demonstrated on a patient suffering from important, uncontrolled pain from a bone metastasis located in the forearm. The 81 × 47 × 34 mm3 lesion was successfully treated using five successive positions of the transducer, under real-time monitoring by MR Thermometry. Significant pain palliation was observed 3 days after the intervention. The system described and characterized in this study is a particularly interesting modular, low-cost MRgHIFU device for musculoskeletal tumor therapy.
Published: 2022
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16. Crucial role of oxygen on the bulk and surface electronic properties of stable β phase of tungsten

Author: Ananya Chattaraj, Sebastien Joulie, Virginie Serin, Alain Claverie, Vijay Kumar, Aloke Kanjilal, Shiv Nadar University, Centre d'élaboration de matériaux et d'études structurales (CEMES), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Matériaux et dispositifs pour l'Electronique et le Magnétisme (CEMES-MEM), and Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse)
Subjects: Multidisciplinary, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci]
Abstract: The A15 β phase of tungsten has recently attracted great interest for spintronic applications due to the finding of giant spin-Hall effect. As β phase is stabilized by oxygen, we have studied the electronic structure of O-doped β-W from first principles calculations. It is found that 20 at.% O-doping makes β phase lower in energy than α-W. These results are in good agreement with energy dispersive X-ray spectroscopy which also shows ~ 16.84 at.% O in 60 nm thick W films. The latter has predominantly β phase as confirmed by grazing incidence X-ray diffraction (XRD). The simulated XRD of bulk β having 15.79 at.% O also agrees with XRD results. Oxygen binds strongly on the surface and affects the Dirac fermion behavior in pure β-W. There is structural disorder, O-inhomogeneity, and higher density-of-states in O-doped β-W at EF compared with pure α. These results are promising to understand the properties of β-W.
Published: 2022
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17. Modular microfluidic system for on-chip extraction, preconcentration and detection of the cytokine biomarker IL-6 in biofluid

Author: Lucile Alexandre, Amel Bendali, Iago Pereiro, Madad Azimani, Simon Dumas, Laurent Malaquin, Thanh Duc Mai, Stéphanie Descroix, Institut Galien Paris-Saclay (IGPS), Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire Physico-Chimie Curie [Institut Curie] (PCC), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC), Institut Pierre-Gilles de Gennes pour la Microfluidique, Macromolécules et Microsystèmes en Biologie et en Médecine [Insitut Curie] (MMBM), Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut Curie [Paris]-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Équipe Ingénierie pour les sciences du vivant (LAAS-ELIA), Laboratoire d'analyse et d'architecture des systèmes (LAAS), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT), and ANR-10-IDEX-0001-02 PSLANR-10-EQPX-34ANR-10-LABX-31CATCH-UDNAINDEX
Subjects: [SPI]Engineering Sciences [physics], Multidisciplinary, Interleukin-6, Lab-On-A-Chip Devices, Microfluidics, Cytokines, Microfluidic Analytical Techniques, Biomarkers
Abstract: The cytokine interleukin 6 (IL-6) is involved in the pathogenesis of different inflammatory diseases, including cancer, and its monitoring could help diagnosis, prognosis of relapse-free survival and recurrence. Here, we report an innovative microfluidic approach that uses the fluidization of magnetic beads to specifically extract, preconcentrate and fluorescently detect IL-6 directly on-chip. We assess how the physical properties of the beads can be tuned to improve assay performance by enhancing mass transport, reduce non-specific binding and multiply the detection signal threefold by transitioning between packed and fluidization states. With the integration of a full ELISA protocol in a single microfluidic chamber, we show a twofold reduction in LOD compared to conventional methods along with a large dynamic range (10 pg/mL to 2 ng/mL). We additionally demonstrate its application to IL-6 detection in undiluted serum samples.
Published: 2022
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18. Depolarization imaging for fast and non-invasive monitoring of cervical microstructure remodeling in vivo during pregnancy

Author: Rehbinder, Jean, Vizet, Jérémy, Park, Junha, Ossikovski, Razvigor, Vanel, Jean-Charles, Nazac, André, Pierangelo, Angelo, Laboratoire des sciences de l'ingénieur, de l'informatique et de l'imagerie (ICube), École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Université de Strasbourg (UNISTRA)-Institut National des Sciences Appliquées - Strasbourg (INSA Strasbourg), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Les Hôpitaux Universitaires de Strasbourg (HUS)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de physique des interfaces et des couches minces [Palaiseau] (LPICM), École polytechnique (X)-Centre National de la Recherche Scientifique (CNRS), and ANR-18-CE19-0007,DigitMC-PB,Colposcopie polarimétrique de Mueller numérique pour la détection à haute performance de l'accouchement prématuré(2018)
Subjects: Diagnostic Imaging, Multidisciplinary, Pregnancy, [INFO.INFO-IM]Computer Science [cs]/Medical Imaging, Infant, Newborn, Parturition, Humans, Premature Birth, Female, Cervix Uteri, Perinatal Mortality
Abstract: The cervix plays a crucial role in conception, maintenance of pregnancy, and childbirth. The mechanical properties of a pregnant woman's cervix change dramatically during gestation due to a remodeling of its microstructure, necessary for delivery. However, external factors can accelerate this process and lead to prematurity, the primary cause of perinatal mortality worldwide, due to the inefficiency of existing diagnostic methods. This study shows that polarized light is a powerful tool to probe the cervical microstructure during pregnancy. A wide-field multispectral polarimetric imaging system was fabricated to explore in vivo the cervix of full-term pregnant women. The polarimetric properties of the cervix change significantly with pregnancy progression. In particular, a set of several depolarization parameters (intrinsic and extrinsic) showed a strong linear correlation with gestational age in the red part of the visible spectral range. This trend can be attributed, among other things, to a decrease in collagen density and an increase in hydration of cervical connective tissue. Wide field depolarization imaging is a very promising tool for rapid and non-invasive analysis of cervical tissue in vivo to monitor the steady progression of pregnancy, providing the practitioner with useful information to improve the detection of preterm birth.
Published: 2022
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19. Molecular principles of recruitment and dynamics of guest proteins in liquid droplets

Author: Virginie Gaudon, Bertrand Castaing, Hiroto Takahashi, Nanako Iwaki, Yaakov Levy, Kiyoto Kamagata, Satoshi Takahashi, Milan K. Hazra, Hiroyuki Oikawa, Trishit Banerjee, Rika Chiba, Saori Kanbayashi, Michiko Kimura, Seiji Sakomoto, Tohoku University [Sendai], Weizmann Institute of Science [Rehovot, Israël], Kyoto University, Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Frapart, Isabelle, Kyoto University [Kyoto], and Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)
Subjects: Protein Folding, Science, [SDV]Life Sciences [q-bio], macromolecular substances, Molecular Dynamics Simulation, Phase Transition, Article, Computational biophysics, 03 medical and health sciences, Molecular dynamics, 0302 clinical medicine, Single-molecule biophysics, Organelle, Fluorescence microscope, Host protein, 030304 developmental biology, Biomolecular Condensates, Organelles, 0303 health sciences, Intrinsically disordered proteins, Multidisciplinary, Chemistry, Dynamics (mechanics), Single Molecule Imaging, [SDV] Life Sciences [q-bio], Microscopy, Fluorescence, Mutation, Biophysics, Medicine, Protein Multimerization, Tumor Suppressor Protein p53, 030217 neurology & neurosurgery
Abstract: Despite the continuous discovery of host and guest proteins in membraneless organelles, complex host–guest interactions hinder the understanding of the molecular grammar governing liquid–liquid phase separation. In this study, we characterized the localization and dynamic properties of guest proteins in liquid droplets using single-molecule fluorescence microscopy. Eighteen guest proteins of different sizes, structures, and oligomeric states were examined in host p53 liquid droplets. Recruitment did not significantly depend on the structural properties of the guest proteins, but was moderately correlated with their length, total charge, and number of R and Y residues. In contrast, the diffusion of disordered guest proteins was comparable to that of host p53, whereas that of folded proteins varied widely. Molecular dynamics simulations suggest that folded proteins diffuse within the voids of the liquid droplet while interacting weakly with neighboring host proteins, whereas disordered proteins adapt their structures to form tight interactions with the host proteins. Our study provides insights into the key molecular principles of the localization and dynamics of guest proteins in liquid droplets.
Published: 2021
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20. Discrimination of rosé wines using shotgun metabolomics with a genetic algorithm and MS ion intensity ratios

Author: Cédric Saucier, Guillaume Cazals, Christine Enjalbal, Mélodie Gil, Robert Sabatier, Christelle Reynes, Sciences Pour l'Oenologie (SPO), Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Biolaffort Company, Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Astruc, Suzette, Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Subjects: 0301 basic medicine, [SDV]Life Sciences [q-bio], lcsh:Medicine, Wine, Mass spectrometry, 01 natural sciences, Mass Spectrometry, Article, Ion, 03 medical and health sciences, Metabolomics, lcsh:Science, Ions, Rose (mathematics), Multidisciplinary, Chromatography, Chemistry, 010401 analytical chemistry, lcsh:R, Polyphenols, Linear discriminant analysis, 0104 chemical sciences, [STAT]Statistics [stat], [SDV] Life Sciences [q-bio], 030104 developmental biology, Mass spectrum, lcsh:Q, Time-of-flight mass spectrometry, Analytical chemistry, Algorithms, Chromatography, Liquid
Abstract: International audience; A rapid Ultra performance Liquid chromatography coupled with Quadrupole/time of flight Mass Spectrometry (UpLc-Qtof-MS) method was designed to quickly acquire high-resolution mass spectra metabolomics fingerprints for rosé wines. An original statistical analysis involving ion ratios, discriminant analysis, and genetic algorithm (GA) was then applied to study the discrimination of rosé wines according to their origins. After noise reduction and ion peak alignments on the mass spectra, about 14 000 different signals were detected. The use of an in-house mass spectrometry database allowed us to assign 72 molecules. Then, a genetic algorithm was applied on two series of samples (learning and validation sets), each composed of 30 commercial wines from three different wine producing regions of France. Excellent results were obtained with only four diagnostic peaks and two ion ratios. This new approach could be applied to other aspects of wine production but also to other metabolomics studies. Wine is a widely consumed alcoholic beverage with a high commercial value. More specifically, the worldwide consumption of rosé wine has increased by 20% since 2002 1. Because of its high commercial value, it can become a subject of fraud, and authenticity control is required in order to maintain wine quality and to detect any adulteration 2. Thousands of molecules can be found in wines, including polyphenols 3. Recently, more than one hundred polyphenols have been quantified in various rosé wines 4. They are key components involved in color, taste and quality of wines. Their amount and composition depend on many different factors such as grape variety, geographic origin, winemaking, age. Several methods have already been developed for wine authentication purpose 5. They can be divided into two categories: metabolite profiling 6-8 or metabolomic fingerprinting 9,10. The first one is a targeted analysis focusing on a limited number of representative components while the second one is a non-targeted approach. Both methods were applied to red or white wines. In a previous work 11 , a very fast UPLC-QTOF-MS method was developed to characterize red wines from different grape varieties. One specific ion ratio was used to discriminate commercial red wines from three grape varieties. In this paper, we focused on the influence of the geographic origin of some rosé French wines. The chemical composition of grapes depends on the sum of different environmental conditions, which can be defined as a "terroir" that should influence the grape and wine composition. The goals of this paper were to develop: • A new and very fast UPLC-QTOF-MS wine metabolomics method with a focus on wine pigments. • An original statistical method and workflow that allow the robust discrimination of rosés wines according to their origins by using mass spectrometry ion ratio fingerprints. Results and Discussions UPLC-QTOF-MS analysis. First, a fast UPLC-QTOF-MS method was developed to rapidly acquire high-resolution mass spectra. In accordance with previous work and conclusions, we have used a short gradient instead of isocratic elution conditions or direct injections 11. It was shown that the last two methods gave limited results probably due to ionization suppression effect. In this work, we chose to work on the positive ionization
Published: 2020
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21. The small molecule inhibitor anle145c thermodynamically traps human islet amyloid peptide in the form of non-cytotoxic oligomers

Author: Saravanan, Manikam S, Ryazanov, Sergey, Leonov, Andrei, Nicolai, Janine, Praest, Patrique, Giese, Armin, Winter, Roland, Khemtemourian, Lucie, Griesinger, Christian, Killian, J Antoinette, Sub Membrane Biochemistry & Biophysics, Membrane Biochemistry and Biophysics, Sub Membrane Biochemistry & Biophysics, Membrane Biochemistry and Biophysics, Utrecht University [Utrecht], Max Planck Institute for Biophysical Chemistry (MPI-BPC), Max-Planck-Gesellschaft, Georg-August-University [Göttingen], Technische Universität Dortmund [Dortmund] (TU), University Medical Center [Utrecht], University-Hospital Munich-Großhadern [München], Laboratoire des biomolécules (LBM UMR 7203), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Chimie Moléculaire de Paris Centre (FR 2769), Institut de Chimie du CNRS (INC)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Chimie et Biologie des Membranes et des Nanoobjets (CBMN), École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut de Chimie du CNRS (INC)-Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), and Université Sciences et Technologies - Bordeaux 1-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Prions, lcsh:Medicine, Peptide, Protein aggregation, 010402 general chemistry, Fibril, 01 natural sciences, Biophysical Phenomena, Article, Cell Line, Small Molecule Libraries, Protein Aggregates, 03 medical and health sciences, Animals, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, oligomers, anle145c, Amino Acid Sequence, Cytotoxicity, Mode of action, General, lcsh:Science, Peptide sequence, chemistry.chemical_classification, geography, Multidisciplinary, geography.geographical_feature_category, Intrinsically disordered proteins, Cell Death, lcsh:R, [CHIM.MATE]Chemical Sciences/Material chemistry, Islet, Small molecule, Islet Amyloid Polypeptide, Rats, 0104 chemical sciences, Kinetics, 030104 developmental biology, chemistry, Biophysics, Thermodynamics, lcsh:Q, Protein Multimerization
Abstract: Type 2 diabetes (T2DM) is associated with aggregation of the human islet amyloid polypeptide (hIAPP) into cytotoxic amyloid species. Here we tested the effect of a diphenylpyrazole (DPP)-derived small molecule inhibitor, anle145c, on cytotoxicity and on aggregation properties of hIAPP. We demonstrate that incubation of hIAPP with the inhibitor yields ~10 nm-sized non-toxic oligomers, independent of the initial aggregation state of hIAPP. This suggests that anle145c has a special mode of action in which anle145c-stabilized oligomers act as a thermodynamic sink for the preferred aggregation state of hIAPP and anle145c. We also demonstrate that the inhibitor acts in a very efficient manner, with sub-stoichiometric concentrations of anle145c being sufficient to (i) inhibit hIAPP-induced death of INS-1E cells, (ii) prevent hIAPP fibril formation in solution, and (iii) convert preformed hIAPP fibrils into non-toxic oligomers. Together, these results indicate that anle145c is a promising candidate for inhibition of amyloid formation in T2DM.
Published: 2019
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22. Three cooperative mechanisms required for recovery after brain damage

Author: Lucilla de Arcangelis, Damian L. Berger, Laurens Michiels van Kessenich, Hans J. Herrmann, Emanuele Varriale, AIR:EAU+JMH, Institut de recherches sur la catalyse et l'environnement de Lyon (IRCELYON), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Berger, D., Varriale, E., Michiels von Kessenich, L., Herrmann, H. J., and de Arcangelis, L.
Subjects: 0301 basic medicine, System recovery, Models, Neurological, Synaptogenesis, lcsh:Medicine, Brain damage, Biology, Article, 03 medical and health sciences, 0302 clinical medicine, medicine, Humans, Statistical physics, thermodynamics and nonlinear dynamics, lcsh:Science, Stroke, ComputingMilieux_MISCELLANEOUS, [PHYS]Physics [physics], Multidisciplinary, Neuronal Plasticity, Mechanism (biology), lcsh:R, Brain, Recovery of Function, medicine.disease, 030104 developmental biology, FOS: Biological sciences, Quantitative Biology - Neurons and Cognition, Brain Injuries, Functional activity, Neurons and Cognition (q-bio.NC), lcsh:Q, medicine.symptom, Neuroscience, Biological physics, 030217 neurology & neurosurgery
Abstract: Stroke is one of the main causes of human disabilities. Experimental observations indicate that several mechanisms are activated during the recovery of functional activity after a stroke. Here we unveil how the brain recovers by explaining the role played by three mechanisms: Plastic adaptation, hyperexcitability and synaptogenesis. We consider two different damages in a neural network: A diffuse damage that simply causes the reduction of the effective system size and a localized damage, a stroke, that strongly alters the spontaneous activity of the system. Recovery mechanisms observed experimentally are implemented both separately and in a combined way. Interestingly, each mechanism contributes to the recovery to a limited extent. Only the combined application of all three together is able to recover the spontaneous activity of the undamaged system. This explains why the brain triggers independent mechanisms, whose cooperation is the fundamental ingredient for the system’s recovery., Scientific Reports, 9 (1), ISSN:2045-2322
Published: 2019
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23. Reprogramming Escherichia coli for the production of prenylated indole diketopiperazine alkaloids

Author: Nicolas Canu, Muriel Gondry, Isabelle Correia, Pascal Belin, Robert Thai, Olivier Lequin, Isabelle Jacques, Pavlina Dubois, Steven G. DuBois, Fabien Le Chevalier, Mireille Moutiez, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire des biomolécules (LBM UMR 7203), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Chimie Moléculaire de Paris Centre (FR 2769), Institut de Chimie du CNRS (INC)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO) (SIMoS), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP), This work was supported by the French Alternative Energies and Atomic Energy Commission (CEA), the French National Centre for Scientific Research (CNRS), the Sorbonne University and the French National Research Agency (grant ANR-16-CE29-0026)., We thank Dr Taek Soon Lee and Melanya Gudzeva from the Lawrence Berkeley National Laboratory for providing us with plasmids pJBEI-3085 and pJBEI-3122., ANR-16-CE29-0026,DKP-COMBIBIO,L'utilisation des voies de biosynthèse dépendant des synthases de cyclodipeptides pour obtenir de nouvelles 2,5-dicétopipérazines bioactives(2016), Chimie Moléculaire de Paris Centre (FR 2769), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Département de Chimie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC), Institute of Neurosciences and Psychology [Glasgow], University of Glasgow, Institut de Neurosciences de la Timone (INT), and Aix Marseille Université (AMU)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Stereochemistry, [SDV]Life Sciences [q-bio], lcsh:Medicine, Context (language use), Diketopiperazines, medicine.disease_cause, Article, Indole Alkaloids, 03 medical and health sciences, 0302 clinical medicine, medicine, Escherichia coli, Moiety, [CHIM]Chemical Sciences, Author Correction, lcsh:Science, 2. Zero hunger, chemistry.chemical_classification, Indole test, Prenylation, Multidisciplinary, Chemistry, Alkaloid, lcsh:R, Bioproduction, 030104 developmental biology, Enzyme, Biocatalysis, lcsh:Q, Mevalonate pathway, Genetic Engineering, Metabolic engineering, 030217 neurology & neurosurgery
Abstract: Prenylated indole diketopiperazine (DKP) alkaloids are important bioactive molecules or their precursors. In the context of synthetic biology, efficient means for their biological production would increase their chemical diversification and the discovery of novel bioactive compounds. Here, we prove the suitability of the Escherichia coli chassis for the production of prenylated indole DKP alkaloids. We used enzyme combinations not found in nature by co-expressing bacterial cyclodipeptide synthases (CDPSs) that assemble the DKP ring and fungal prenyltransferases (PTs) that transfer the allylic moiety from the dimethylallyl diphosphate (DMAPP) to the indole ring of tryptophanyl-containing cyclodipeptides. Of the 11 tested combinations, seven resulted in the production of eight different prenylated indole DKP alkaloids as determined by LC-MS/MS and NMR characterization. Two were previously undescribed. Engineering E. coli by introducing a hybrid mevalonate pathway for increasing intracellular DMAPP levels improved prenylated indole DKP alkaloid production. Purified product yields of 2–26 mg/L per culture were obtained from culture supernatants. Our study paves the way for the bioproduction of novel prenylated indole DKP alkaloids in a tractable chassis that can exploit the cyclodipeptide diversity achievable with CDPSs and the numerous described PT activities.
Published: 2019
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24. Ligand induced switching of the band alignment in aqueous synthesized CdTe/CdS core/shell nanocrystals

Author: Jefferson Luis Ferrari, Rafael S. Mourão, Brener R. C. Vale, Dmitry Aldakov, Marco Antônio Schiavon, J. C. L. Sousa, Peter Reiss, Jefferson Bettini, Universidade Federal de São João del-Rei (UFSJ), Brazilian Nanotechnol Natl Lab, Campinas, SP, Brazil, Department of Engenharias [Vila Real], Universidade de Trás-os-Montes e Alto Douro (UTAD), Laboratoire d'Electronique Moléculaire Organique et Hybride (LEMOH), SYstèmes Moléculaires et nanoMatériaux pour l’Energie et la Santé (SYMMES), Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Synthèse, Structure et Propriétés de Matériaux Fonctionnels (STEP), Department of Botany and Plant Sciences, University of California, Centro Nacional de Pesquisa em Energia e Materiais = Brazilian Center for Research in Energy and Materials (CNPEM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG)
Subjects: 0301 basic medicine, Photoluminescence, Materials science, lcsh:Medicine, [CHIM.INOR]Chemical Sciences/Inorganic chemistry, Article, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, X-ray photoelectron spectroscopy, Thioglycolic acid, Carboxylate, Fluorescence spectroscopy, lcsh:Science, ComputingMilieux_MISCELLANEOUS, Multidisciplinary, Aqueous solution, Ligand, Quantum dots, lcsh:R, [CHIM.MATE]Chemical Sciences/Material chemistry, Crystallography, 030104 developmental biology, Sulfonate, chemistry, Scanning probe microscopy, Quantum dot, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Nanoparticles, lcsh:Q, [CHIM.OTHE]Chemical Sciences/Other, 030217 neurology & neurosurgery
Abstract: CdTe/CdS core/shell quantum dots (QDs) are formed in aqueous synthesis via the partial decomposition of hydrophilic thiols, used as surface ligands. In this work, we investigate the influence of the chemical nature (functional group and chain length) of the used surface ligands on the shell formation. Four different surface ligands are compared: 3-mercaptopropionic acid, MPA, thioglycolic acid, TGA, sodium 3-mercaptopropanesulfonate, MPS, and sodium 2-mercaptoethanesulfonate, MES. The QD growth rate increases when the ligand aliphatic chain length decreases due to steric reasons. At the same time, the QDs stabilized with carboxylate ligands grow faster and achieve higher photoluminescence quantum yields compared to those containing sulfonate ligands. The average PL lifetime of TGA and MPA capped QDs is similar (≈20 ns) while in the case of MPS shorter (≈15 ns) and for MES significantly longer (≈30 ns) values are measured. A detailed structural analysis combining powder X-ray diffraction, and X-ray photoelectron spectroscopy (XPS) indicates the existence of two novel regimes of band alignment: in the case of the mercaptocarboxylate ligands the classic type I band alignment between the core and shell materials is predominant, while the mercaptosulfonate ligands induce a quasi-type II alignment (MES) or an inverted type I alignment (MPS). Finally, the effect of the pH value on the optical properties was evaluated: using a ligand excess in solution allows achieving better stability of the QDs while maintaining high photoluminescence intensity at low pH.
Published: 2019
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25. Photonic jet lens

Author: Lecler, Sylvain, Perrin, Stéphane, Leong-Hoi, Audrey, Montgomery, Paul, Laboratoire des sciences de l'ingénieur, de l'informatique et de l'imagerie (ICube), École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Université de Strasbourg (UNISTRA)-Institut National des Sciences Appliquées - Strasbourg (INSA Strasbourg), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Les Hôpitaux Universitaires de Strasbourg (HUS)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174) (FEMTO-ST), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Institut d'Electronique du Solide et des Systèmes (InESS), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées - Strasbourg (INSA Strasbourg), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université de Technologie de Belfort-Montbeliard (UTBM)-Ecole Nationale Supérieure de Mécanique et des Microtechniques (ENSMM)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), and PERRIN, Stéphane
Subjects: [PHYS.PHYS.PHYS-OPTICS] Physics [physics]/Physics [physics]/Optics [physics.optics], [PHYS.PHYS.PHYS-OPTICS]Physics [physics]/Physics [physics]/Optics [physics.optics], [SPI.OPTI] Engineering Sciences [physics]/Optics / Photonic, lcsh:R, lcsh:Medicine, Article, [SPI]Engineering Sciences [physics], [PHYS.PHYS.PHYS-INS-DET] Physics [physics]/Physics [physics]/Instrumentation and Detectors [physics.ins-det], [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, lcsh:Q, [PHYS.PHYS.PHYS-INS-DET]Physics [physics]/Physics [physics]/Instrumentation and Detectors [physics.ins-det], lcsh:Science, [SPI.SIGNAL]Engineering Sciences [physics]/Signal and Image processing, ComputingMilieux_MISCELLANEOUS, [SPI.SIGNAL] Engineering Sciences [physics]/Signal and Image processing
Abstract: Microsphere-assisted microscopy currently benefits from a considerable interest in the microscope-research community. Indeed, this new imaging technique enables the lateral resolution of optical microscopes to reach around λ/5 through a full-field and a far-field acquisition while being label-free. Despite the photonic jet clearly not being a relevant concept to justify the super-resolution phenomenon, we show here how it can be used to predict imaging formation and performance such as the image position and the microsphere magnification. This study allows a better understanding of the experimental measurements that have been observed over the last decade and that will be observed in coming years, through numerical simulations using different optical and geometrical parameters.
Published: 2019
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26. IR GRIN lenses prepared by ionic exchange in chalcohalide glasses

Author: Xianghua Zhang, Mathieu Rozé, Thierry Pain, Enora Lavanant, Laurent Calvez, Francis Gouttefangeas, Yann Guimond, Claire Fourmentin, Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), Umicore IR Glass, Synthèse Caractérisation Analyse de la Matière (ScanMAT), Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut de Chimie du CNRS (INC), The authors are grateful to the French DGA, Direction Générale de l’Armement (project ANR-18-ASTR-0014) for supporting this work. Authors would like to acknowledge the IUF (Institut Universitaire de France). This publication is supported by the European Union through the European Regional Development Fund (ERDF), the Ministry of Higher Education and Research, the french region of Brittany and Rennes Métropole., ANR-18-ASTR-0014,IRGRIN,Optiques InfraRouges moulable à GRadient d'INdice de réfraction à base de verres de chalcogénures(2018), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université de Rennes (UR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Materials for devices, Materials science, Infrared, Chalcogenide, Science, Diffusion, Analytical chemistry, Halide, Ionic bonding, 02 engineering and technology, 01 natural sciences, Article, 010309 optics, chemistry.chemical_compound, 0103 physical sciences, [CHIM]Chemical Sciences, Multidisciplinary, Ion exchange, Glasses, 021001 nanoscience & nanotechnology, Alkali metal, chemistry, Medicine, 0210 nano-technology, Refractive index, Materials for optics
Abstract: In order to decrease the number of lenses and the weight of thermal imaging devices, specific optical design are required by using gradient refractive index (GRIN) elements transparent in the infrared waveband. While widely used for making visible GRIN lenses with silicate glasses, the ion exchange process is very limited when applied to chalcogenide glasses due to their low Tg and relatively weak mechanical properties. In this paper, we develop chalco-halide glasses based on alkali halide (NaI) addition in a highly covalent GeSe2–Ga2Se3 matrix, efficient for tailoring a significant and permanent change of refractive by ion exchange process between K+ and Na+. Optical and structural properties of the glass samples were measured showing a diffusion length reaching more than 2 mm and a Gaussian gradient of refractive index Δn of 4.5.10–2. The obtained GRIN lenses maintain an excellent transmission in the second (3–5 µm) and third (8–12 µm) atmospheric windows.
Published: 2021
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27. Prediction of coating thickness for polyelectrolyte multilayers via machine learning

Author: Gribova, Varvara, Navalikhina, Anastasiia, Lysenko, Oleksandr, Calligaro, Cynthia, Lebaudy, Eloïse, Deiber, Lucie, Senger, Bernard, Lavalle, Philippe, Vrana, Nihal Engin, Biomatériaux et Bioingénierie (BB), Université de Strasbourg (UNISTRA)-Matériaux et nanosciences d'Alsace (FMNGE), and Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Biomaterials, [CHIM.POLY]Chemical Sciences/Polymers, [CHIM.GENI]Chemical Sciences/Chemical engineering, [INFO.INFO-LG]Computer Science [cs]/Machine Learning [cs.LG], Science, Medicine, Nanobiotechnology, Article
Abstract: Layer-by-layer (LbL) deposition method of polyelectrolytes is a versatile way of developing functional nanoscale coatings. Even though the mechanisms of LbL film development are well-established, currently there are no predictive models that can link film components with their final properties. The current health crisis has shown the importance of accelerated development of biomedical solutions such as antiviral coatings, and the implementation of machine learning methodologies for coating development can enable achieving this. In this work, using literature data and newly generated experimental results, we first analyzed the relative impact of 23 coating parameters on the coating thickness. Next, a predictive model has been developed using aforementioned parameters and molecular descriptors of polymers from the DeepChem library. Model performance was limited because of insufficient number of data points in the training set, due to the scarce availability of data in the literature. Despite this limitation, we demonstrate, for the first time, utilization of machine learning for prediction of LbL coating properties. It can decrease the time necessary to obtain functional coating with desired properties, as well as decrease experimental costs and enable the fast first response to crisis situations (such as pandemics) where coatings can positively contribute. Besides coating thickness, which was selected as an output value in this study, machine learning approach can be potentially used to predict functional properties of multilayer coatings, e.g. biocompatibility, cell adhesive, antibacterial, antiviral or anti-inflammatory properties.
Published: 2021
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28. Long-term evolution of the epithelial cell secretome in preclinical 3D models of the human bronchial epithelium

Author: Sanchez-Guzman, Daniel, Boland, Sonja, Brookes, Oliver, Mc Cord, Claire, Lai Kuen, René, Sirri, Valentina, Baeza Squiban, Armelle, Devineau, Stéphanie, Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPC), Plates-formes mutualisées du centre de recherche pharmaceutique de Paris (P-MIM - UMS 3612), Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPC), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), Institut de Recherche pour le Développement (IRD)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP), and Devineau, Stéphanie
Subjects: Proteomics, Cell biology, Proteome, Science, [SDV]Life Sciences [q-bio], Cell Culture Techniques, Bronchi, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, Models, Biological, Article, Mass Spectrometry, Cell Line, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Humans, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Principal Component Analysis, SARS-CoV-2, COVID-19, Epithelial Cells, Culture Media, Respiratory system models, Medicine, [SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Angiotensin-Converting Enzyme 2
Abstract: International audience; Abstract The human bronchial epithelium is the first line of defense against atmospheric particles, pollutants, and respiratory pathogens such as the novel SARS-CoV-2. The epithelial cells form a tight barrier and secrete proteins that are major components of the mucosal immune response. Functional in vitro models of the human lung are essential for screening the epithelial response and assessing the toxicity and barrier crossing of drugs, inhaled particles, and pollutants. However, there is a lack of models to investigate the effect of chronic exposure without resorting to animal testing. Here, we developed a 3D model of the human bronchial epithelium using Calu-3 cell line and demonstrated its viability and functionality for 21 days without subculturing. We investigated the effect of reduced Fetal Bovine Serum supplementation in the basal medium and defined the minimal supplementation needed to maintain a functional epithelium, so that the amount of exogenous serum proteins could be reduced during drug testing. The long-term evolution of the epithelial cell secretome was fully characterized by quantitative mass spectrometry in two preclinical models using Calu-3 or primary NHBE cells. 408 common secreted proteins were identified while significant differences in protein abundance were observed with time, suggesting that 7–10 days are necessary to establish a mature secretome in the Calu-3 model. The associated Reactome pathways highlight the role of the secreted proteins in the immune response of the bronchial epithelium. We suggest this preclinical 3D model can be used to evaluate the long-term toxicity of drugs or particles on the human bronchial epithelium, and subsequently to investigate their effect on the epithelial cell secretions.
Published: 2021
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29. Synthesis of silver nanostructure on gold nanoparticle using near field assisted second harmonic generation

Author: Benjamin Leuschel, Olivier Soppera, Takashi Yatsui, Felix Julian Brandenburg, Toyohashi University of Technology (TUT ), Institut de Science des Matériaux de Mulhouse (IS2M), Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Soppera, Olivier, Centre National de la Recherche Scientifique (CNRS)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)
Subjects: Nanostructure, Materials science, [SPI.OPTI] Engineering Sciences [physics]/Optics / Photonic, Science, Nanoparticle, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, 7. Clean energy, Article, Plasmon, [CHIM.MATE] Chemical Sciences/Material chemistry, Nanophotonics and plasmonics, Multidisciplinary, business.industry, Second-harmonic generation, [CHIM.MATE]Chemical Sciences/Material chemistry, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Wavelength, Absorption edge, Transmission electron microscopy, [SPI.OPTI]Engineering Sciences [physics]/Optics / Photonic, Medicine, Optoelectronics, Nanoparticles, 0210 nano-technology, business, Photoinitiator
Abstract: By using gold (Au) nanoparticles (NPs) as an optical near-field source under far-field illumination in combination with a silver (Ag) ion solution containing a photoinitiator, we coated Ag on Au NPs using a near-field (NF)-assisted process. We evaluated the change in the size of the NPs using transmission electron microscopy. Evaluation of the synthesized Ag volume over illumination power confirmed the squared power dependence of the NP volume with illumination using 808 nm light, i.e., a wavelength longer than the absorption edge wavelength of the photoinitiator molecules. The rate of volume increase was much lower than the plasmonic field enhancement effect. Therefore, the squared power dependency of the volume increase using a wavelength longer than the absorption edge wavelength originated from NF-assisted second-harmonic generation and the resulting excitation.
Published: 2021
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30. IgG acquisition against PfEMP1 PF11_0521 domain cassette DC13, DBLβ3_D4 domain, and peptides located within these constructs in children with cerebral malaria

Author: Badaut, Cyril, Visitdesotrakul, Pimnitah, Chabry, Aurelie, Bigey, Pascal, Tornyigah, Bernard, Roman, Jocelyne, Maroufou, Jules Alao, Amoussou, Annick, Ayivi, Blaise Serge, Sagbo, Gratien, Ndam, Nicaise Tuikue, Oleinikov, Andrew V., Tahar, Rachida, Institut de Recherche Biomédicale des Armées [Antenne Marseille] (IRBA), Florida Atlantic University [Boca Raton], Mère et enfant en milieu tropical : pathogènes, système de santé et transition épidémiologique (MERIT - UMR_D 216), Institut de Recherche pour le Développement (IRD)-Université de Paris (UP), Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS - UM 4 (UMR 8258 / U1022)), Institut de Chimie du CNRS (INC)-Université de Paris (UP)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Universitaire de la Mère et de l'Enfant Lagune (CHU-MEL), Centre National Hospitalier Universitaire Hubert K. Maga de Cotonou (CNHU-HKM), R01AI092120, National Institutes of Health, ANR-11-IDEX-0005-02, National French Agency of Research, Mère et enfant en milieu tropical : pathogènes, système de santé et transition épidémiologique (MERIT - UMR_D 261), Institut de Recherche pour le Développement (IRD)-Université Paris Cité (UPCité), Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), and ORANGE, Colette
Subjects: BLOOD-STAGE ANTIGENS, PLASMODIUM-FALCIPARUM, IDENTIFICATION, RECEPTOR, Molecular biology, Science, ERYTHROCYTE-MEMBRANE PROTEIN-1, INFECTED ERYTHROCYTES, Immunology, Article, ANTIBODIES, BINDING, Medicine, NATURALLY ACQUIRED-IMMUNITY, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, PROTECTION, [SDV.MP.PAR] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology
Abstract: International audience; The Plasmodium falciparum erythrocyte-membrane-protein-1 (PF3D7_1150400/PF11_0521) contains both domain cassette DC13 and DBL beta 3 domain binding to EPCR and ICAM-1 receptors, respectively. This type of PfEMP1 proteins with dual binding specificity mediate specific interactions with brain micro-vessels endothelium leading to the development of cerebral malaria (CM). Using plasma collected from children at time of hospital admission and after 30 days, we study an acquisition of IgG response to PF3D7_1150400/PF11_0521 DC13 and DBL beta 3_D4 recombinant constructs, and five peptides located within these constructs, specifically in DBL alpha 1.7_D2 and DBL beta 3_D4 domains. We found significant IgG responses against the entire DC13, PF11_0521_DBL beta 3_D4 domain, and peptides. The responses varied against different peptides and depended on the clinical status of children. The response was stronger at day 30, and mostly did not differ between CM and uncomplicated malaria (UM) groups. Specifically, the DBL beta 3 B3-34 peptide that contains essential residues involved in the interaction between PF11_0521 DBL beta 3_D4 domain and ICAM-1 receptor demonstrated significant increase in reactivity to IgG1 and IgG3 antibodies at convalescence. Further, IgG reactivity in CM group at time of admission against functionally active (ICAM-1-binding) PF11_0521 DBL beta 3_D4 domain was associated with protection against severe anemia. These results support development of vaccine based on the PF3D7_1150400/PF11_0521 structures to prevent CM.
Published: 2021
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31. More than redox, biological organic ligands control iron isotope fractionation in the riparian wetland

Author: Martine Bouhnik-Le Coz, Anne-Catherine Pierson-Wickmann, Olivier Rouxel, Rémi Marsac, Elaheh Lotfi-Kalahroodi, Mélanie Davranche, Khalil Hanna, Géosciences Rennes (GR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), C-FACTOR (ANR-18-CE01-0008), Agence Nationale de la Recherche, ISO-TRANS-FER, INSU EC2CO, ANR-18-CE01-0008,C-FACTOR,Le devenir des contaminants est contrôlé par la spéciation de la matière organique colloïdale(2018), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: chemistry.chemical_classification, Multidisciplinary, Isotope, Chemistry, Science, Fractionation, Biogeochemistry, 010501 environmental sciences, Sedimentation, 010502 geochemistry & geophysics, 01 natural sciences, Redox, Article, Environmental sciences, Isotopic signature, Isotope fractionation, [SDU.STU.GC]Sciences of the Universe [physics]/Earth Sciences/Geochemistry, Environmental chemistry, Medicine, Organic matter, Riparian wetland, 0105 earth and related environmental sciences
Abstract: Although redox reactions are recognized to fractionate iron (Fe) isotopes, the dominant mechanisms controlling the Fe isotope fractionation and notably the role of organic matter (OM) are still debated. Here, we demonstrate how binding to organic ligands governs Fe isotope fractionation beyond that arising from redox reactions. The reductive biodissolution of soil Fe(III) enriched the solution in light Fe isotopes, whereas, with the extended reduction, the preferential binding of heavy Fe isotopes to large biological organic ligands enriched the solution in heavy Fe isotopes. Under oxic conditions, the aggregation/sedimentation of Fe(III) nano-oxides with OM resulted in an initial enrichment of the solution in light Fe isotopes. However, heavy Fe isotopes progressively dominate the solution composition in response to their binding with large biologically-derived organic ligands. Confronted with field data, these results demonstrate that Fe isotope systematics in wetlands are controlled by the OM flux, masking Fe isotope fractionation arising from redox reactions. This work sheds light on an overseen aspect of Fe isotopic fractionation and calls for a reevaluation of the parameters controlling the Fe isotopes fractionation to clarify the interpretation of the Fe isotopic signature.
Published: 2021
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32. Molecular basis of the interaction of the human tyrosine phosphatase PTPN3 with the hepatitis B virus core protein

Author: Genera, Mariano, Quioc-Salomon, Barbara, Nourisson, Antonin, Colcombet-Cazenave, Baptiste, Haouz, Ahmed, Mechaly, Ariel, Matondo, Mariette, Duchateau, Magalie, König, Alexander, Windisch, Marc, Neuveut, Christine, Wolff, Nicolas, Caillet-Saguy, Célia, ED 515 - Complexité du vivant, Sorbonne Université (SU), Récepteurs Canaux - Channel Receptors, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Hépacivirus et Immunité innée, Université Paris Diderot - Paris 7 (UPD7), Cristallographie (Plateforme) - Crystallography (Platform), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Plateforme de Protéomique / Proteomics platform, Spectrométrie de Masse pour la Biologie – Mass Spectrometry for Biology (UTechS MSBio), Institut Pasteur [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris]-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut Pasteur Korea - Institut Pasteur de Corée, Réseau International des Instituts Pasteur (RIIP), MG is part of the Pasteur-Paris University (PPU) International PhD Program. BQS was supported by ANRS. BCC was supported by the Ministère de l'Enseignement Supérieur et de la Recherche., We thank the staff of the crystallography platform at Institut Pasteur for carrying out robot-driven crystallization screening. We thank Sébastien Brûlé and Bertrand Raynal from the Institut Pasteur molecular biophysics platform for the design and analysis of the AUC experiment. We thank Bruno Vitorge from the Institut Pasteur biological NMR technological platform for help with NMR experiments. We acknowledge synchrotron SOLEIL (Saint-Aubin, France) for granting access to their facility and the staff of Proxima1 for helpful assistance during the data collection. We thank Lauriane Lecoq and Anja Böckmann for the pRSF-T7-HBc183opt plasmid and the advices to produce HBc., Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), and Institut Pasteur [Paris] (IP)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris] (IP)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Hepatitis B virus, Molecular biology, Science, viruses, PDZ Domains, Biochemistry, Article, Capsid, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Structure determination, Biophysical methods, Viral Core Proteins, Biological techniques, Protein Tyrosine Phosphatase, Non-Receptor Type 3, High-throughput screening, virus diseases, Proteins, Hepatitis B, Hepatitis B Core Antigens, digestive system diseases, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Medicine, Tyrosine, Protein Tyrosine Phosphatases
Abstract: International audience; Interactions between the hepatitis B virus core protein (HBc) and host cell proteins are poorly understood, although they may be essential for the propagation of the virus and its pathogenicity. HBc has a C-terminal PDZ (PSD-95, Dlg1, ZO-1)-binding motif (PBM) that is responsible for interactions with host PDZ domain-containing proteins. In this work, we focused on the human protein tyrosine phosphatase non-receptor type 3 (PTPN3) and its interaction with HBc. We solved the crystal structure of the PDZ domain of PTPN3 in complex with the PBM of HBc, revealing a network of interactions specific to class I PDZ domains despite the presence of a C-terminal cysteine in this atypical PBM. We further showed that PTPN3 binds the HBc protein within capsids or as a homodimer. We demonstrate that overexpression of PTPN3 significantly affects HBV infection in HepG2 NTCP cells. Finally, we performed proteomics studies on both sides by pull-down assays and screening of a human PDZ domain library. We identified a pool of human PBM-containing proteins that might interact with PTPN3 in cells and that could be in competition with the HBc PBM during infection, and we also identified potential cellular partners of HBc through PDZ-PBM interactions. This study opens up many avenues of future investigations into the pathophysiology of HBV.
Published: 2021
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33. Experimental protection of quantum coherence by using a phase-tunable image drive

Author: Hervé Vezin, Irinel Chiorescu, Sylvain Bertaina, H. De Raedt, Institut des Matériaux, de Microélectronique et des Nanosciences de Provence (IM2NP), Aix Marseille Université (AMU)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 (LASIRe), Ecole Nationale Supérieure de Chimie de Lille (ENSCL)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Florida State University [Tallahassee] (FSU), Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 (LASIRE), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Université de Lille, CNRS, Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 [LASIRE], Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Centrale Lille Institut (CLIL), and Zernike Institute for Advanced Materials
Subjects: Floquet theory, DECOHERENCE, Dynamical decoupling, media_common.quotation_subject, Science, Fidelity, FOS: Physical sciences, 02 engineering and technology, 01 natural sciences, Article, [PHYS.QPHY]Physics [physics]/Quantum Physics [quant-ph], Quantum mechanics, 0103 physical sciences, Mesoscale and Nanoscale Physics (cond-mat.mes-hall), Quantum information, Hardware_ARITHMETICANDLOGICSTRUCTURES, Condensed-matter physics, 010306 general physics, Quantum, Quantum computer, media_common, Physics, Quantum Physics, Multidisciplinary, Condensed Matter - Mesoscale and Nanoscale Physics, 021001 nanoscience & nanotechnology, Qubit, Medicine, [PHYS.COND.CM-SCE]Physics [physics]/Condensed Matter [cond-mat]/Strongly Correlated Electrons [cond-mat.str-el], 0210 nano-technology, Quantum Physics (quant-ph), Qubits, Coherence (physics)
Abstract: The protection of qubit coherence is an essential task in order to build a practical quantum computer able to manipulate, store and read quantum information with a high degree of fidelity. Recently, it has been proposed to increase the operation time of a qubit by means of strong pulses to achieve a dynamical decoupling of the qubit from its environment. We propose and demonstrate a simple and highly efficient alternative pulse protocol based on Floquet modes, which increases the decoherence time in a number of materials with different spin Hamiltonians and environments. We demonstrate the regime $T_2\approx T_1$, thus providing a route for spin qubits and spin ensembles to be used in quantum information processing and storage., Supplementary informations at the end of the file
Published: 2020
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34. Effect of electrolyte flow on a gas evolution electrode

Author: Alexis Grimaud, Nicolas Dubouis, Gwenaëlle Toussaint, Philippe Stevens, Soufiane Abdelghani-Idrissi, Annie Colin, Chimie-Biologie-Innovation (UMR 8231) (CBI), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Laboratoire des Matériels Électriques (EDF R&D LME), EDF R&D (EDF R&D), EDF (EDF)-EDF (EDF), Chimie du solide et de l'énergie (CSE), Collège de France (CdF (institution))-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Réseau sur le stockage électrochimique de l'énergie (RS2E), Université de Picardie Jules Verne (UPJV)-Institut de Chimie du CNRS (INC)-Aix Marseille Université (AMU)-Université de Pau et des Pays de l'Adour (UPPA)-Université de Nantes (UN)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Collège de France (CdF (institution))-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Université Grenoble Alpes (UGA)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Nantes (UN)-Aix Marseille Université (AMU)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Collège de France (CdF (institution))-Université de Picardie Jules Verne (UPJV)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-Université Grenoble Alpes (UGA), and ANR-17-CE07-0040,FLUIDIDENSE,Suspensions concentrées : vers des matériaux plus fluides et plus denses(2017)
Subjects: Materials science, Energy storage, Electrolytic cell, Science, Energy science and technology, Flow (psychology), 02 engineering and technology, Electrolyte, 010402 general chemistry, 7. Clean energy, 01 natural sciences, Article, Batteries, [CHIM]Chemical Sciences, Multidisciplinary, Gas evolution reaction, Mechanics, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Power (physics), Scientific method, Electrode, Medicine, Electric power, 0210 nano-technology
Abstract: In this study, the effect of flow of the electrolyte on an electrolysis cell and a zinc cell is investigated. The gain of energy brought by the flow is discussed and compared to the viscous losses in the cells. We point out that the balance between the gained electrical power and the viscous loss power is positive only if the hydrodynamic resistance of the circuit is correctly designed and further comment on the economical viability of the whole process. A model of the studied phenomena is proposed in the last section. This analytical model captures the dynamics of the process, gives the optimal flowing conditions and the limits of the energetical rentability of the process. This study shows that the use of flowing electrolyte in zinc–air batteries can be energetically profitable with the appropriate flowing conditions.
Published: 2020
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35. Physical properties of epilithic river biofilm as a new lead to perform pollution bioassessments in overseas territories

Author: Estelle Lefrancois, Alexandra Jestin, Cédric Hubas, Jean-Louis Mansot, Théo Risser, Pascal-Jean Lopez, Hélène Budzinski, Dominique Monti, Laurence Romana, Philippe Keith, Olivier Gros, Béatrice Lauga, Xavier Lourenço, Nathalie Tapie, Alexandre Haouisée, Farid Begarin, Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Station de Biologie Marine de Concarneau, Direction générale déléguée à la Recherche, à l’Expertise, à la Valorisation et à l’Enseignement-Formation (DGD.REVE), Muséum national d'Histoire naturelle (MNHN)-Muséum national d'Histoire naturelle (MNHN), Centre Commun de Caractérisation des Matériaux des Antilles et de la Guyane (C3MAG), Université des Antilles (UA), Groupe de Technologie des Surfaces et Interfaces (GTSI), ECO in-EAU, Fonctionnement agroécologique et performances des systèmes de cultures horticoles (UPR HORTSYS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Environnements et Paléoenvironnements OCéaniques (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Université de Pau et des Pays de l'Adour (UPPA), Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Pau et des Pays de l'Adour (UPPA), Centre National de la Recherche Scientifique (CNRS), Muséum national d'Histoire naturelle (MNHN), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE), Groupe de Technologie des Surfaces et Interfaces [URp2_2] (GTSI), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and ANR-16-IDEX-0002,E2S,E2S(2016)
Subjects: 0106 biological sciences, 0301 basic medicine, Pollution, Science, media_common.quotation_subject, 030106 microbiology, 01 natural sciences, Freshwater ecosystem, Article, Environmental impact, 03 medical and health sciences, Lead (geology), Environmental impact assessment, 14. Life underwater, Environmental quality, media_common, Multidisciplinary, Ecology, 010604 marine biology & hydrobiology, Biofilm, 15. Life on land, Contamination, Tropical ecology, 13. Climate action, [SDE]Environmental Sciences, Medicine, Environmental science
Abstract: International audience; Chlordecone (CLD) levels measured in the rivers of the French West Indies were among the highest values detected worldwide in freshwater ecosystems, and its contamination is recognised as a severe health, environmental, agricultural, economic, and social issue. In these tropical volcanic islands, rivers show strong originalities as simplified food webs, or numerous amphidromous migrating species, making the bioindication of contaminations a difficult issue. The objective of this study was to search for biological responses to CLD pollution in a spatially fixed and long-lasting component of the rivers in the West Indies: the epilithic biofilm. Physical properties were investigated through complementary analyses: friction, viscosity as well as surface adhesion were analyzed and coupled with measures of biofilm carbon content and exopolymeric substance (EPS) production. Our results have pointed out a mesoscale chemical and physical reactivity of the biofilm that can be correlated with CLD contamination. We were able to demonstrate that epilithic biofilm physical properties can effectively be used to infer freshwater environmental quality of French Antilles rivers. The friction coefficient is reactive to contamination and well correlated to carbon content and EPS production. Monitoring biofilm physical properties could offer many advantages to potential users in terms of effectiveness and ease of use, rather than more complex or time-consuming analyses. Out of the 11,435 French water bodies analysed for quality compliance according to regulations imposed by European legislation, 10% are located between the tropics and the equator, thousands of kilometres away from European shores 1. In the outermost tropical territories, climatic characteristics often result in highly turbulent tropical streams 2 that support simplified ecosystems with very few primary producers. Due to high river flow, perennial phytoplankton, zooplankton and macroalgae are scarce or missing and the epilithic biofilm deserves here, even more than elsewhere, the comparison with a real productive and contributive "microbial skin" 3. The epilithic biofilm is the only endogenous long-lasting primary producer that grows on submersed river stones and is largely exploited as a food source by all diadromous fish or crustaceans 4,5. In these countries, the simplified food webs and the massive flows of post-larvae and juveniles regularly re-entering the rivers and migrating
Published: 2020
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36. Deficiency of the SMOC2 matricellular protein impairs bone healing and produces age-dependent bone loss

Author: Supawich Morkmued, Karen Niederreither, François Clauss, Bon-Kyoung Koo, Agnès Bloch-Zupan, Joseph Hemmerlé, Hans Clevers, Pascal Dollé, Eric Mathieu, Valérie Fraulob, Brigitte Schuhbaur, Hubrecht Institute for Developmental Biology and Stem Cell Research, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Khon Kaen University [Thailand] (KKU), Nanomédecine Régénérative (NanoRegMed), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Fédération de Médecine Translationnelle de Strasbourg (FMTS), Université de Strasbourg (UNISTRA), Les Hôpitaux Universitaires de Strasbourg (HUS), Biomatériaux et Bioingénierie (BB), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Utrecht University [Utrecht], University College of London [London] (UCL), and univOAK, Archive ouverte
Subjects: Male, 0301 basic medicine, medicine.medical_specialty, Dentin Matrix Acidic Phosphoprotein 1, lcsh:Medicine, Dental diseases, Bone healing, Development, Real-Time Polymerase Chain Reaction, Article, Bone resorption, Mice, 03 medical and health sciences, 0302 clinical medicine, stomatognathic system, Osteoclast, Internal medicine, [SDV.BDD] Life Sciences [q-bio]/Development Biology, medicine, Animals, Humans, RNA, Messenger, Bone Resorption, lcsh:Science, Bone, [SDV.BDD]Life Sciences [q-bio]/Development Biology, Dental alveolus, Multidisciplinary, Chemistry, Dentin dysplasia, Calcium-Binding Proteins, lcsh:R, Matricellular protein, Gene Expression Regulation, Developmental, medicine.disease, stomatognathic diseases, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Matrix Metalloproteinase 9, Cyclooxygenase 2, 030220 oncology & carcinogenesis, Microscopy, Electron, Scanning, lcsh:Q, Tooth Avulsion, Tooth
Abstract: Secreted extracellular matrix components which regulate craniofacial development could be reactivated and play roles in adult wound healing. We report a patient with a loss-of-function of the secreted matricellular protein SMOC2 (SPARC related modular calcium binding 2) presenting severe oligodontia, microdontia, tooth root deficiencies, alveolar bone hypoplasia, and a range of skeletal malformations. Turning to a mouse model, Smoc2-GFP reporter expression indicates SMOC2 dynamically marks a range of dental and bone progenitors. While germline Smoc2 homozygous mutants are viable, tooth number anomalies, reduced tooth size, altered enamel prism patterning, and spontaneous age-induced periodontal bone and root loss are observed in this mouse model. Whole-genome RNA-sequencing analysis of embryonic day (E) 14.5 cap stage molars revealed reductions in early expressed enamel matrix components (Odontogenic ameloblast-associated protein) and dentin dysplasia targets (Dentin matrix acidic phosphoprotein 1). We tested if like other matricellular proteins SMOC2 was required for regenerative repair. We found that the Smoc2-GFP reporter was reactivated in adjacent periodontal tissues 4 days after tooth avulsion injury. Following maxillary tooth injury, Smoc2−/− mutants had increased osteoclast activity and bone resorption surrounding the extracted molar. Interestingly, a 10-day treatment with the cyclooxygenase 2 (COX2) inhibitor ibuprofen (30 mg/kg body weight) blocked tooth injury-induced bone loss in Smoc2−/− mutants, reducing matrix metalloprotease (Mmp)9. Collectively, our results indicate that endogenous SMOC2 blocks injury-induced jaw bone osteonecrosis and offsets age-induced periodontal decay.
Published: 2020
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37. New results with regard to the Flora bust controversy: radiocarbon dating suggests nineteenth century origin

Author: Ingrid Caffy, Ina Reiche, Lucile Beck, Institut de Recherche de Chimie Paris (IRCP), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Ministère de la Culture (MC), Centre de recherche et de restauration des musées de France (C2RMF), Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de mesure du carbone 14 (LMC14 - UMS 2572), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut de Radioprotection et de Sûreté Nucléaire (IRSN)-Ministère de la Culture et de la Communication (MCC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences du Climat et de l'Environnement [Gif-sur-Yvette] (LSCE), Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ), Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture (MC), and Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, 010506 paleontology, Flora, media_common.quotation_subject, Science, Art history, Italian Renaissance, [SHS.MUSEO]Humanities and Social Sciences/Cultural heritage and museology, 01 natural sciences, Article, law.invention, 03 medical and health sciences, Portrait, Reservoir effect, law, 14. Life underwater, Radiocarbon dating, 0105 earth and related environmental sciences, media_common, Multidisciplinary, Soft materials, The Renaissance, Art, [CHIM.MATE]Chemical Sciences/Material chemistry, [SHS.ART]Humanities and Social Sciences/Art and art history, Biogeochemistry, 030104 developmental biology, Absolute (philosophy), Bust, Medicine
Abstract: Many works of art have been attributed to Leonardo da Vinci (1452–1519), the great artist-scientist-engineer of the Italian Renaissance; however, art historians have struggled to find definitive proof to connect Leonardo to these art pieces. The Flora wax bust in the Bode Museum, Berlin, was attributed to Leonardo because her face resembles several Leonardo portraits, but this attribution has the subject of intense debate since the bust’s acquisition in 1909. Using new chemical analyses and absolute 14C dating, we are able to resolve the question of authenticity. We show that the Flora wax bust is made primarily of spermaceti which was extracted from sperm whales. Therefore, 14C dating must consider the Marine Reservoir Effect. We have generated a new calibration method and dated the bust to the 19th c. This proves that the bust was not produced during the Renaissance, and thus cannot be attributed to da Vinci, and illustrates that 14C dating can be applied to unusual materials.
Published: 2020
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38. Neurostimulation stabilizes spiking neural networks by disrupting seizure-like oscillatory transitions

Author: Taufik A. Valiante, Jérémie Lefebvre, Frances K. Skinner, Scott Rich, Axel Hutt, Krembil Research Institute, University Health Network, Computational Anatomy and Simulation for Medicine (MIMESIS), Inria Nancy - Grand Est, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), University of Toronto, University of Ottawa [Ottawa], We thank the National Research Council of Canada (NSERC grants RGPIN-2017-06662 and RGPIN-2016-06182) and the Krembil Foundation (Krembil Seed Grant and Krembil Postdoctoral Fellowship) for support of this research., Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Laboratoire des sciences de l'ingénieur, de l'informatique et de l'imagerie (ICube), École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Université de Strasbourg (UNISTRA)-Institut National des Sciences Appliquées - Strasbourg (INSA Strasbourg), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Les Hôpitaux Universitaires de Strasbourg (HUS)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Université de Strasbourg (UNISTRA)-Institut National des Sciences Appliquées - Strasbourg (INSA Strasbourg), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Les Hôpitaux Universitaires de Strasbourg (HUS)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), and Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Computer science, medicine.medical_treatment, Models, Neurological, lcsh:Medicine, Action Potentials, Electric Stimulation Therapy, Inhibitory postsynaptic potential, Article, 03 medical and health sciences, Seizure onset, Neural activity, Epilepsy, 0302 clinical medicine, Seizures, Dynamical systems, medicine, Humans, lcsh:Science, Neurostimulation, 030304 developmental biology, Spiking neural network, Neurons, 0303 health sciences, Multidisciplinary, Network models, High frequency stimulation, lcsh:R, Models, Theoretical, medicine.disease, [INFO.INFO-MO]Computer Science [cs]/Modeling and Simulation, Computational neuroscience, Excitatory postsynaptic potential, lcsh:Q, Neural Networks, Computer, Neuroscience, 030217 neurology & neurosurgery
Abstract: An improved understanding of the mechanisms underlying neuromodulatory approaches to mitigate seizure onset is needed to identify clinical targets for the treatment of epilepsy. Using a Wilson–Cowan-motivated network of inhibitory and excitatory populations, we examined the role played by intrinsic and extrinsic stimuli on the network’s predisposition to sudden transitions into oscillatory dynamics, similar to the transition to the seizure state. Our joint computational and mathematical analyses revealed that such stimuli, be they noisy or periodic in nature, exert a stabilizing influence on network responses, disrupting the development of such oscillations. Based on a combination of numerical simulations and mean-field analyses, our results suggest that high variance and/or high frequency stimulation waveforms can prevent multi-stability, a mathematical harbinger of sudden changes in network dynamics. By tuning the neurons’ responses to input, stimuli stabilize network dynamics away from these transitions. Furthermore, our research shows that such stabilization of neural activity occurs through a selective recruitment of inhibitory cells, providing a theoretical undergird for the known key role these cells play in both the healthy and diseased brain. Taken together, these findings provide new vistas on neuromodulatory approaches to stabilize neural microcircuit activity.
Published: 2020
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39. Chemical evidence of rare porphyrins in purple shells of Crassostrea gigas oyster

Author: Bruno Boury, Sonia Cantel, Michel Bonnard, Isabelle Parrot, Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)
Subjects: 0106 biological sciences, 0301 basic medicine, Chlorophyll, Oyster, animal structures, Magnetic Resonance Spectroscopy, Porphyrins, Evolution, lcsh:Medicine, Color, 010603 evolutionary biology, 01 natural sciences, Oyster shells, Article, 03 medical and health sciences, Animal Shells, biology.animal, [CHIM]Chemical Sciences, Animals, Crassostrea, lcsh:Science, ComputingMilieux_MISCELLANEOUS, Chromatography, High Pressure Liquid, Chromatography, Reverse-Phase, Multidisciplinary, biology, Chemistry, lcsh:R, fungi, biology.organism_classification, Chemical biology, Materials science, Environmental sciences, 030104 developmental biology, Ocean sciences, Biochemistry, Tetrapyrroles, Turacin, Luminescent Measurements, Microscopy, Electron, Scanning, lcsh:Q
Abstract: The colour of oyster shells is a very diverse characteristic morphotype, forming intriguing vivid patterns both on the inside and outside of the shell. In the present study, we have identified for the first time, the presence of several porphyrins as constituents of the shell pigmentation of the Crassostrea gigas oyster consumed worldwide. The precise molecular structures of halochromic, fluorescent and acid-soluble porphyrins, such as uroporphyrin and turacin, are unambiguously determined by reverse phase liquid chromatography combined with high resolution mass spectrometry. Their presence account for the purple colouration of shells but also for the dark colouration of adductor muscle scars. We have also defined the endogenous origin of these porphyrins, specifically secreted or accumulated by the shell forming tissue. These findings are pioneering analytical proofs of the existence of the haem pathway in the edible oyster Crassostrea gigas, evidenced by the chemical identification of haem side-products and supported by the recent publication of the corresponding oyster genome.
Published: 2020
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40. Nanoscale 3D quantitative imaging of 1.88 Ga Gunflint microfossils reveals novel insights into taphonomic and biogenic characters

Author: Manuel Guizar-Sicairos, Felisa Berenguer, Loïc Bertrand, Plinio Jaqueto, Pierre Gueriau, Keyron Hickman-Lewis, Frances Westall, Ricardo I.F. Trindade, Douglas Galante, André L. Rossi, Mariana Verezhak, Lara Maldanis, Laboratório Nacional de Luz Sìncrotron (LNLS), Centro Nacional de Pesquisa em Energia e Materiais = Brazilian Center for Research in Energy and Materials (CNPEM), Instituto de Física de São Carlos (IFSC-USP), Universidade de São Paulo = University of São Paulo (USP), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO), Paul Scherrer Institute (PSI), Institut photonique d'analyse non-destructive européen des matériaux anciens (IPANEMA), Muséum national d'Histoire naturelle (MNHN)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture (MC), Institute of Earth Sciences [Lausanne], Université de Lausanne = University of Lausanne (UNIL), Centro Brasileiro de Pesquisas Físicas (CBPF), Ministério da Ciência e Tecnologia, Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Photophysique et Photochimie Supramoléculaires et Macromoléculaires (PPSM), Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Ecole Normale Supérieure Paris-Saclay (ENS Paris Saclay), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, CNPq: 301263/2017–5, 424367/2016–5 Fundaçao de Amparo Ã Pesquisa do Estado de Sao Paulo, FAPESP H2020 Marie Skłodowska-Curie Actions, MSCA: 701647 Fundaçao de Amparo Ã Pesquisa do Estado de Sao Paulo, FAPESP: 2015/21810–6, 2016/25681–9 European Commission, EC: 654028 Coordenaçao de Aperfeiçoamento de Pessoal de NÃvel Superior, CAPES Instituto Serrapilheira: Serra-1709–20205 Centre National d'Etudes Spatiales, CNES Coordenaçao de Aperfeiçoamento de Pessoal de NÃvel Superior, CAPES Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung, SNSF: 200021L_169753 Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, CNPq 607297 Centre National de la Recherche Scientifique, CNRS, We acknowledge the Paul Scherrer Institut, Villigen, Switzerland for provision of synchrotron radiation beamtime at cSAXS beamline of the Swiss Light Source (SLS), Joakim Reuteler (ScopeM) for the preparation of the samples using FIB, the Centro Brasileiro de Pesquisas Físicas (CBPF) for support in sample preparation and SEM analysis, and Sylvain Janiec (ISTO, Orléans) for preparing the thin sections. LM was funded by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001 and by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP grants 2015/21810–6 and 2016/25681–9), which included being hosted at the IPANEMA laboratory. DG acknowledges FAPESP (2016/06114–6), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, 424367/2016–5 and 301263/2017–5). This work was supported by the Serrapilheira Institute (grant number Serra-1709–20205). KHL and FW acknowledge the support of the French National Centre for Scientific Research (CNRS), the French Space Agency (CNES) and the MASE (Mars Analogues for Space Exploration) Project (EU-FP7 Grant no. 607297). IPANEMA and the SOLEIL synchrotron are supported by the Research Infrastructures activity IPERION CH of the Horizon2020 program of the European Commission (Grant Agreement No. 654028). LB acknowledges support from Région Île-de-France / DIM Matériaux anciens et patrimoniaux. MV is supported by European Union’s Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No 701647, as well as SNSF grant No 200021L_169753. We also acknowledge the constructive comments of three anonymous reviewers., European Project: 654028,H2020 Pilier Excellent Science,H2020-INFRAIA-2014-2015,IPERION CH(2015), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), Universidade de São Paulo (USP), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), HAL UVSQ, Équipe, and Integrated Platform for the European Research Infrastructure ON Cultural Heritage - IPERION CH - - H2020 Pilier Excellent Science2015-05-01 - 2019-04-30 - 654028 - VALID
Subjects: [CHIM.ANAL] Chemical Sciences/Analytical chemistry, Taphonomy, Science, Geochemistry, Context (language use), 02 engineering and technology, 010502 geochemistry & geophysics, 01 natural sciences, Article, Precambrian, chemistry.chemical_compound, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, synchrotron, ptychography, Kerogen, 0105 earth and related environmental sciences, fossil, Multidisciplinary, Proterozoic, Physics, Biota, Biogeochemistry, 15. Life on land, Hematite, 021001 nanoscience & nanotechnology, [PHYS.COND.CM-MS] Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], ESPECTROSCOPIA, Diagenesis, chemistry, visual_art, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], visual_art.visual_art_medium, Medicine, [SDU.STU.PG] Sciences of the Universe [physics]/Earth Sciences/Paleontology, [SDU.STU.PG]Sciences of the Universe [physics]/Earth Sciences/Paleontology, 0210 nano-technology, paleontology, diagenesis, Geology
Abstract: Precambrian cellular remains frequently have simple morphologies, micrometric dimensions and are poorly preserved, imposing severe analytical and interpretational challenges, especially for irrefutable attestations of biogenicity. The 1.88 Ga Gunflint biota is a Precambrian microfossil assemblage with different types and qualities of preservation across its numerous geological localities and provides important insights into the Proterozoic biosphere and taphonomic processes. Here we use synchrotron-based ptychographic X-ray computed tomography to investigate well-preserved carbonaceous microfossils from the Schreiber Beach locality as well as poorly-preserved, iron-replaced fossil filaments from the Mink Mountain locality, Gunflint Formation. 3D nanoscale imaging with contrast based on electron density allowed us to assess the morphology and carbonaceous composition of different specimens and identify the minerals associated with their preservation based on retrieved mass densities. In the Mink Mountain filaments, the identification of mature kerogen and maghemite rather than the ubiquitously described hematite indicates an influence from biogenic organics on the local maturation of iron oxides through diagenesis. This non-destructive 3D approach to microfossil composition at the nanoscale within their geological context represents a powerful approach to assess the taphonomy and biogenicity of challenging or poorly preserved traces of early microbial life, and may be applied effectively to extraterrestrial samples returned from upcoming space missions.
Published: 2020
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41. Topographical curvature is sufficient to control epithelium elongation

Author: Rafaela Silva dos Santos, Karine Anselme, Laurent Pieuchot, Julie Marteau, Maxence Bigerelle, Marcos Farina, P.-F. Chauvy, Pablo Rougerie, Laboratoire d'Automatique, de Mécanique et d'Informatique industrielles et Humaines - UMR 8201 (LAMIH), Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-INSA Institut National des Sciences Appliquées Hauts-de-France (INSA Hauts-De-France), Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Institut de Science des Matériaux de Mulhouse (IS2M), Centre National de la Recherche Scientifique (CNRS)-Matériaux et nanosciences d'Alsace (FMNGE), Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Micropat SA, Anselme, Karine, Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), and Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)
Subjects: 0301 basic medicine, Cell biology, Biomaterial design, [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, Surface Properties, Morphogenesis, lcsh:Medicine, 02 engineering and technology, Cell Growth Processes, [SPI.MAT] Engineering Sciences [physics]/Materials, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Curvature, Kidney, Article, [SPI.MAT]Engineering Sciences [physics]/Materials, Madin Darby Canine Kidney Cells, Biomaterials, [PHYS.MECA.MEMA]Physics [physics]/Mechanics [physics]/Mechanics of materials [physics.class-ph], 03 medical and health sciences, Dogs, Tissue engineering, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], medicine, Animals, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, lcsh:Science, Groove (engineering), ComputingMilieux_MISCELLANEOUS, geography, Multidisciplinary, geography.geographical_feature_category, lcsh:R, Cell Differentiation, Epithelial Cells, 021001 nanoscience & nanotechnology, Epithelium, 030104 developmental biology, medicine.anatomical_structure, Ridge, Biophysics, lcsh:Q, Elongation, 0210 nano-technology
Abstract: How biophysical cues can control tissue morphogenesis is a central question in biology and for the development of efficient tissue engineering strategies. Recent data suggest that specific topographies such as grooves and ridges can trigger anisotropic tissue growth. However, the specific contribution of biologically relevant topographical features such as cell-scale curvature is still unclear. Here we engineer a series of grooves and ridges model topographies exhibiting specific curvature at the ridge/groove junctions and monitored the growth of epithelial colonies on these surfaces. We observe a striking proportionality between the maximum convex curvature of the ridges and the elongation of the epithelium. This is accompanied by the anisotropic distribution of F-actin and nuclei with partial exclusion of both in convex regions as well as the curvature-dependent reorientation of pluricellular protrusions and mitotic spindles. This demonstrates that curvature itself is sufficient to trigger and modulate the oriented growth of epithelia through the formation of convex “topographical barriers” and establishes curvature as a powerful tuning parameter for tissue engineering and biomimetic biomaterial design.
Published: 2020
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42. Synthesis and Kinetic evaluation of an azido analogue of methylerythritol phosphate: a Novel Inhibitor of E. coli YgbP/IspD

Author: Jean-Luc Ferrer, Philippe Chaignon, Alain Wagner, Franck Borel, Zoljargal Baatarkhuu, Myriam Seemann, Institut de Chimie de Strasbourg, Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie structurale (IBS - UMR 5075 ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université de Strasbourg - Faculté de Médecine [Strabourg] (FMTS), Université Louis Pasteur - Strasbourg I-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Université Louis Pasteur - Strasbourg I-Institut de Chimie du CNRS (INC)
Subjects: 0301 basic medicine, Models, Molecular, Plasmodium falciparum, lcsh:Medicine, Drug resistance, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, medicine.disease_cause, 01 natural sciences, Article, Phosphates, 03 medical and health sciences, chemistry.chemical_compound, medicine, Escherichia coli, Humans, Enzyme Inhibitors, lcsh:Science, chemistry.chemical_classification, Multidisciplinary, biology, 010405 organic chemistry, Escherichia coli Proteins, lcsh:R, Phosphate, biology.organism_classification, Antimicrobial, Drug Resistance, Multiple, 3. Good health, 0104 chemical sciences, Multiple drug resistance, Kinetics, 030104 developmental biology, Enzyme, Erythritol, chemistry, Biochemistry, Sugar Phosphates, lcsh:Q, Bacteria
Abstract: As multidrug resistant pathogenic microorganisms are a serious health menace, it is crucial to continuously develop novel medicines in order to overcome the emerging resistance. The methylerythritol phosphate pathway (MEP) is an ideal target for antimicrobial development as it is absent in humans but present in most bacteria and in the parasite Plasmodium falciparum. Here, we report the synthesis and the steady-state kinetics of a novel potent inhibitor (MEPN3) of Escherichia coli YgbP/IspD, the third enzyme of the MEP pathway. MEPN3 inhibits E. coli YgbP/IspD in mixed type mode regarding both substrates. Interestingly, MEPN3 shows the highest inhibitory activity when compared to known inhibitors of E. coli YgbP/IspD. The mechanism of this enzyme was also studied by steady-state kinetic analysis and it was found that the substrates add to the enzyme in sequential manner.
Published: 2018
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43. Deciphering the molecular basis of mycobacteria and lipoglycan recognition by the C-type lectin Dectin-2

Author: Decout, Alexiane, Silva-Gomes, Sandro, Drocourt, Daniel, Blattes, Emilyne, Rivière, Michel, Prandi, Jacques, Larrouy-Maumus, Gérald, Caminade, Anne-Marie, Hamasur, Beston, Källenius, Gunilla, Kaur, Devinder, Dobos, Karen M., Lucas, Megan, Sutcliffe, Iain C., Besra, Gurdyal S., Appelmelk, Ben J., Gilleron, Martine, Jackson, Mary, Vercellone, Alain, Tiraby, Gérard, Nigou, Jérôme, Institut de pharmacologie et de biologie structurale (IPBS), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, InvivoGen Europe, Laboratoire de chimie de coordination (LCC), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Karolinska Institutet [Stockholm], Biopromic AB, Department of Microbiology, Immunology and Pathology, University of Colorado [Boulder], Faculty of Health and Life Sciences, University of Northumbria at Newcastle [United Kingdom], Institute of Microbiology and Infection, University of Birmingham [Birmingham], Department of Medical Microbiology and Infection Control, VU University Medical Center [Amsterdam], Medical Microbiology and Infection Prevention, AII - Infectious diseases, Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), and Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Lipopolysaccharides, BACILLUS-CALMETTE-GUERIN, PHOSPHATIDYLINOSITOL MANNOSIDE, lcsh:Medicine, Ligands, DC-SIGN, [SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity, Article, INNATE IMMUNE-SYSTEM, Animals, Humans, Lectins, C-Type, lcsh:Science, PRO-INFLAMMATORY ACTIVITY, TUBERCULOSIS H37RV, CANDIDA-ALBICANS, Science & Technology, Cell Membrane, MYO-INOSITOL ANCHOR, lcsh:R, C500, Mycobacterium tuberculosis, CARBOHYDRATE-RECOGNITION, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Mice, Inbred C57BL, Multidisciplinary Sciences, Science & Technology - Other Topics, LIPOARABINOMANNAN, lcsh:Q, Signal Transduction
Abstract: International audience; Dectin-2 is a C-type lectin involved in the recognition of several pathogens such as Aspergillus fumigatus, Candida albicans, Schistosoma mansonii, and Mycobacterium tuberculosis that triggers Th17 immune responses. Identifying pathogen ligands and understanding the molecular basis of their recognition is one of the current challenges. Purified M. tuberculosis mannose-capped lipoarabinomannan (ManLAM) was shown to induce signaling via Dectin-2, an activity that requires the (α1 → 2)-linked mannosides forming the caps. Here, using isogenic M. tuberculosis mutant strains, we demonstrate that ManLAM is a bona fide and actually the sole ligand mediating bacilli recognition by Dectin-2, although M. tuberculosis produces a variety of cell envelope mannoconjugates, such as phosphatidyl-myo-inositol hexamannosides, lipomannan or manno(lipo)proteins, that bear (α1 → 2)-linked mannosides. In addition, we found that Dectin-2 can recognize lipoglycans from other bacterial species, such as Saccharotrix aerocolonigenes or the human opportunistic pathogen Tsukamurella paurometabola, suggesting that lipoglycans are prototypical Dectin-2 ligands. Finally, from a structure/function relationship perspective, we show, using lipoglycan variants and synthetic mannodendrimers, that dimannoside caps and multivalent interaction are required for ligand binding to and signaling via Dectin-2. Better understanding of the molecular basis of ligand recognition by Dectin-2 will pave the way for the rational design of potent adjuvants targeting this receptor.
Published: 2018
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44. The absence of SigX results in impaired carbon metabolism and membrane fluidity in Pseudomonas aeruginosa

Author: Ali Tahrioui, Maud Flechard, Sylvie Chevalier, Cecil Onyedikachi Azuama, Eric Déziel, Gwendoline Gicquel, Cécile Duclairoir-Poc, Rachel Duchesne, Manuella Catel-Ferreira, Emeline Bouffartigues, Thomas Clamens, Marie-Christine Groleau, Damien Tortuel, Coralie Lagy, Ségolène Depayras, Marc G. J. Feuilloley, Hermann J. Heipieper, Pierre Cornelis, Olivier Maillot, Olivier Lesouhaitier, Julie Hardouin, Jonchère, Laurent, Interactions cellulaires et moléculaires (ICM), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Microbiologie Signaux et Microenvironnement (LMSM), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Laboratoire de Microbiologie du Froid – Signaux et Micro-Environnement (LMDF-SME), Microbiologie de l'Eau (MDE), Ecologie et biologie des interactions (EBI), Université de Poitiers-Centre National de la Recherche Scientifique (CNRS)-Université de Poitiers-Centre National de la Recherche Scientifique (CNRS), Polymères Biopolymères Surfaces (PBS), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Helmholtz Zentrum für Umweltforschung = Helmholtz Centre for Environmental Research (UFZ), Institut Armand Frappier (INRS-IAF), Institut National de la Recherche Scientifique [Québec] (INRS)-Réseau International des Instituts Pasteur (RIIP), Laboratory of Microbial Interactions, Université de Brussel, Vriendenkring VUB, Microbiology, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), and Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Catabolite Repression, 0301 basic medicine, Proteome, Membrane Fluidity, Mutant, Catabolite repression, lcsh:Medicine, Sigma Factor, [SDV.TOX.TCA]Life Sciences [q-bio]/Toxicology/Toxicology and food chain, [SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Article, Cell wall, 03 medical and health sciences, chemistry.chemical_compound, Bacterial Proteins, Lysogeny broth, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Membrane fluidity, lcsh:Science, ComputingMilieux_MISCELLANEOUS, Regulation of gene expression, Multidisciplinary, Chemistry, Gene Expression Profiling, lcsh:R, Biological Transport, Gene Expression Regulation, Bacterial, Metabolism, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Phenotype, Carbon, Cell biology, 030104 developmental biology, [SDV.TOX.TCA] Life Sciences [q-bio]/Toxicology/Toxicology and food chain, general, Pseudomonas aeruginosa, lcsh:Q, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Energy Metabolism
Abstract: In Pseudomonas aeruginosa, SigX is an extra-cytoplasmic function σ factor that belongs to the cell wall stress response network. In previous studies, we made the puzzling observation that sigX mutant growth was severely affected in rich lysogeny broth (LB) but not in minimal medium. Here, through comparative transcriptomic and proteomic analysis, we show that the absence of SigX results in dysregulation of genes, whose products are mainly involved in transport, carbon and energy metabolisms. Production of most of these genes is controlled by carbon catabolite repression (CCR), a key regulatory system than ensures preferential carbon source uptake and utilization, substrate prioritization and metabolism. The strong CCR response elicited in LB was lowered in a sigX mutant, suggesting altered nutrient uptake. Since the absence of SigX affects membrane composition and fluidity, we suspected membrane changes to cause such phenotype. The detergent polysorbate 80 (PS80) can moderately destabilize the envelope resulting in non-specific increased nutrient intake. Remarkably, growth, membrane fluidity and expression of dysregulated genes in the sigX mutant strain were restored in LB supplemented with PS80. Altogether, these data suggest that SigX is indirectly involved in CCR regulation, possibly via its effects on membrane integrity and fluidity.
Published: 2018
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45. Clerodane furanoditerpenoids as the probable cause of toxic hepatitis induced by Tinospora crispa

Author: Sylvie Michel, Chouaha Bouzidi, Denis Boucaud-Maitre, Cyril Colas, Xavier Cachet, Ludivine Riffault-Valois, Jérôme Langrand, Annabelle Dugay, Equipe Pharmacognosie (UMR 8638), Chimie Organique, Médicinale et Extractive et Toxicologie Expérimentale (COMETE - UMR 8638), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS), Centre antipoison de Paris, Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Université Paris Descartes - Paris 5 (UPD5)-Centre National de la Recherche Scientifique (CNRS), Centre de biophysique moléculaire (CBM), Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Direction de la recherche clinique et de l'innovation CHU Guadeloupe, Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Centre antipoison et de toxicovigilance (Paris) (CAPTV Paris), Université Paris Diderot - Paris 7 (UPD7)-Hôpitaux Universitaires Saint-Louis, Lariboisière, Fernand-Widal, Université Paris Descartes - Paris 5 (UPD5)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université d'Orléans (UO)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)
Subjects: 0301 basic medicine, Toxic hepatitis, Male, Tinospora, Dioscorea bulbifera, Electrospray ionization, lcsh:Medicine, Context (language use), Article, Diterpenes, Clerodane, Teucrium, 03 medical and health sciences, 0302 clinical medicine, Liver Function Tests, Borapetoside C, medicine, Humans, lcsh:Science, ComputingMilieux_MISCELLANEOUS, Multidisciplinary, biology, Traditional medicine, Plant Stems, Chemistry, Plant Extracts, lcsh:R, Middle Aged, biology.organism_classification, 3. Good health, 030104 developmental biology, Phytochemical, Liver, 030220 oncology & carcinogenesis, [SHS.ENVIR]Humanities and Social Sciences/Environmental studies, lcsh:Q, medicine.symptom, Chemical and Drug Induced Liver Injury, Tinospora crispa
Abstract: Tinospora crispa is a popular traditional herbal plant commonly used throughout the world for treatment of various diseases, in particular type 2 diabetes mellitus. We report here a new case of toxic hepatitis in a 57-year old male patient in the French West Indies following the consumption of two aqueous extracts of fresh Tinospora crispa stems. It thus differs from two previously reported cases that concerned the chronic intake of powdered dry stems delivered in solid oral dosage forms (i.e. pellets and tablets). Liquid Chromatography-Diode Array Detection-Mass Spectrometry (LC/DAD/MS) analyses were performed on an aqueous extract of the offending sample that mimics the swallowed preparation. They revealed the presence of species-specific molecular marker borapetoside C (1) and thus enabled an unambiguous phytochemical identification. The exploration of tandem MS/MS data obtained by ultra-high performance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC-ESI-QTOF-HRMS) allowed the identification of 17 additional cis-clerodane-type furanoditerpenoid lactones, analogues of 1. These results support the hypothesis that the mechanisms underlying hepatotoxicity of Tinospora crispa are the same as those encountered with furanoditerpenoids-containing plants such as Teucrium chamaedrys or Dioscorea bulbifera. In the context of type 2 diabetes treatment, we recommend that Tinospora crispa intake should be more closely monitored for signs of hepatotoxicity.
Published: 2018
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46. The inducible chemical-genetic fluorescent marker FAST outperforms classical fluorescent proteins in the quantitative reporting of bacterial biofilm dynamics

Author: Monmeyran, Amaury, Thomen, Philippe, Jonquière, Hugo, Sureau, Franck, Li, Chenge, Plamont, Marie-Aude, Douarche, Carine, Casella, Jean-François, Gautier, Arnaud, Henry, Nelly, Laboratoire Jean Perrin (LJP), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Processus d'Activation Sélective par Transfert d'Energie Uni-électronique ou Radiatif (UMR 8640) (PASTEUR), Université Pierre et Marie Curie - Paris 6 (UPMC)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Département de Chimie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physique des Solides (LPS), Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Biologie Paris Seine (IBPS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Val de Loire Recherche en Management (VALLOREM), and Université d'Orléans (UO)-Université de Tours
Subjects: [PHYS.PHYS.PHYS-BIO-PH]Physics [physics]/Physics [physics]/Biological Physics [physics.bio-ph], Green Fluorescent Proteins, lcsh:R, lcsh:Medicine, Article, Luminescent Proteins, Microscopy, Fluorescence, Genes, Reporter, Biofilms, [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB], Escherichia coli, lcsh:Q, lcsh:Science, ComputingMilieux_MISCELLANEOUS
Abstract: International audience; To increase our understanding of bacterial biofilm complexity, real-time quantitative analyses of the living community functions are required. To reach this goal, accurate fluorescent reporters are needed. In this paper, we used the classical fluorescent genetic reporters of the GFP family and demonstrated their limits in the context of a living biofilm. We showed that fluorescence signal saturated after only a few hours of growth and related this saturation to the reduction of oxygen concentration induced by bacterial consumption. This behaviour prevents the use of GFP-like fluorescent proteins for quantitative measurement in living biofilms. To overcome this limitation, we propose the use of a recently introduced small protein tag, FAST, which is fluorescent in the presence of an exogenously applied fluorogenic dye, enabling to avoid the oxygen sensitivity issue. We compared the ability of FAST to report on biofilm growth with that of GFP and mCherry, and demonstrated the superiority of the FAST:fluorogen probes for investigating dynamics in the complex environment of a living biofilm.
Published: 2018
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47. Magnetic-field induced rotation of magnetosome chains in silicified magnetotactic bacteria

Author: Blondeau, Marine, Guyodo, Yohan, Guyot, François, Gatel, Christophe, Menguy, Nicolas, Chebbi, Imène, Haye, Bernard, Durand-Dubief, Mickaël, Alphandery, Edouard, Brayner, Roberta, Coradin, Thibaud, Institut de minéralogie, de physique des matériaux et de cosmochimie (IMPMC), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de recherche pour le développement [IRD] : UR206-Centre National de la Recherche Scientifique (CNRS), Matériaux et Biologie (LCMCP-MATBIO), Laboratoire de Chimie de la Matière Condensée de Paris (LCMCP), Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Institut de minéralogie et de physique des milieux condensés (IMPMC), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Diderot - Paris 7 (UPD7)-Institut de Physique du Globe de Paris (IPG Paris)-Centre National de la Recherche Scientifique (CNRS), Interférométrie, In situ et Instrumentation pour la Microscopie Electronique (CEMES-I3EM), Centre d'élaboration de matériaux et d'études structurales (CEMES), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Institut National des Sciences Appliquées (INSA)-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie de Toulouse (ICT-FR 2599), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Nanobactérie SARL, Acides nucléiques : dynamique, ciblage, et fonctions biologiques - Régulation et dynamique des génomes (ANDCFB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS (UMR_7086)), Université Paris Diderot - Paris 7 (UPD7)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Trichet, Lea, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de recherche pour le développement [IRD] : UR206-Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Université Pierre et Marie Curie - Paris 6 (UPMC)-IPG PARIS-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut de Chimie du CNRS (INC)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse III - Paul Sabatier (UT3), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut de Chimie de Toulouse (ICT), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), and Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Magnetic Fields, [CHIM] Chemical Sciences, lcsh:R, [CHIM]Chemical Sciences, lcsh:Medicine, lcsh:Q, Magnetosomes, Magnetospirillum, Silicon Dioxide, lcsh:Science, Article
Abstract: International audience; Understanding the biological processes enabling magnetotactic bacteria to maintain oriented chains of magnetic iron-bearing nanoparticles called magnetosomes is a major challenge. The study aimed to constrain the role of an external applied magnetic field on the alignment of magnetosome chains in Magnetospirillum magneticum AMB-1 magnetotactic bacteria immobilized within a hydrated silica matrix. A deviation of the chain orientation was evidenced, without significant impact on cell viability, which was preserved after the field was turned-off. Transmission electron microscopy showed that the crystallographic orientation of the nanoparticles within the chains were preserved. Off-axis electron holography evidenced that the change in magnetosome orientation was accompanied by a shift from parallel to anti-parallel interactions between individual nanocrystals. The field-induced destructuration of the chain occurs according to two possible mechanisms: (i) each magnetosome responds individually and reorients in the magnetic field direction and/or (ii) short magnetosome chains deviate in the magnetic field direction. This work enlightens the strong dynamic character of the magnetosome assembly and widens the potentialities of magnetotactic bacteria in bionanotechnology. Since their discovery more than 30 years ago, magnetotactic bacteria have received much attention in the fields of geological, chemical, physical and biological sciences 1-5. However, the processes by which they synthesize iron oxide (or sulphide) magnetic nanoparticles and organize them into chains allowing the cell to orient itself along the geomagnetic field are still far from being fully understood 6-10. It is well-admitted that production of the nano-crystals occurs in specialized organelles, called magnetosomes, formed by a membrane invagination at multiple sites in the cell. However, identifying the proteins controlling nucleation, size, shape and arrangement in chains of the nanocrystals, as well as the mechanisms of action of these proteins, remains a challenging task 11-15. Phenotypic divergences between different species were evidenced 16. In some Magnetospirillum sp. strains, the chain is stabilized along the longitudinal axis of the cell by an organic filament, MamK protein, while the connection of magnetosomes to this structure is provided by a protein MamJ 17,18. The deletion of MamK gene in Magnetospirillum gryphiswaldense MSR-1 leads to short chains separated by gaps devoid of magnetosomes, while the deletion of MamJ induces aggregation of magnetosomes within the cell with observable filaments not attached to the biogenic particles 19,20. In Magnetospirillum magneticum AMB-1, additional MamJ-like and MamK-like proteins were identified that are involved in the dynamic of the filament and the magnetosome organization in chain 21-23. The capacity of the magnetosome chain to act as a compass is not only related to the spatial arrangement of nanocrystals, but also to their crystallographic and magnetic alignment. In some magnetotactic bacteria (e.g., Magnetospirillum sp.), the biogenic magnetite nanoparticles have a cubo-octahedral morphology with a magnetic
Published: 2018
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48. Gas and seismicity within the Istanbul seismic gap

Author: Geli L., Henry P., Grall C., Tary J.-B., Lomax A., Batsi E., Riboulot V., Cros E., Gurbuz C., Islk S.E., Sengor A.M.C., Le Pichon X., Ruffine L., Dupre S., Thomas Y., Kalafat D., Bayrakci G., Coutellier Q., Regnier T., Westbrook G., Saritas H., Cifci G., Cagatay M.N., Ozeren M.S., Gorur N., Tryon M., Bohnhoff M., Gasperini L., Klingelhoefer F., Scalabrin C., Augustin J.-M., Embriaco D., Marinaro G., Frugoni F., Monna S., Etiope G., Favali P., Becel A., Institut Français de Recherche pour l'Exploitation de la Mer - Brest ( IFREMER ), Institut Français de Recherche pour l'Exploitation de la Mer ( IFREMER ), Centre européen de recherche et d'enseignement de géosciences de l'environnement ( CEREGE ), Centre National de la Recherche Scientifique ( CNRS ) -Institut de Recherche pour le Développement ( IRD ) -Aix Marseille Université ( AMU ) -Collège de France ( CdF ) -Institut National de la Recherche Agronomique ( INRA ) -Institut national des sciences de l'Univers ( INSU - CNRS ), Lamont-Doherty Earth Observatory ( LDEO ), Columbia University [New York], University of Alberta [Edmonton], Unité de recherche Géosciences Marines (Ifremer) ( GM ), IFREMER, Institut des Sciences Chimiques de Rennes ( ISCR ), Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ) -Université de Rennes ( UNIV-RENNES ) -Ecole Nationale Supérieure de Chimie de Rennes-Institut National des Sciences Appliquées ( INSA ) -Centre National de la Recherche Scientifique ( CNRS ), Department of Earth and Environmental Sciences, University of Birmingham [Birmingham], Dokuz Eylul University, Institute of Marine Sciences and Technology, Dokuz Eylül University, Istanbul Technical University, GeoForschungsZentrum - Helmholtz-Zentrum Potsdam ( GFZ ), Istituto di Science Marine - ISMAR (Italy), Istituto Nazionale di Geofisica e Vulcanologia, Institut de Physique du Globe de Paris ( IPGP ), Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -IPG PARIS-Université Paris Diderot - Paris 7 ( UPD7 ) -Université de la Réunion ( UR ) -Centre National de la Recherche Scientifique ( CNRS ), Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Centre européen de recherche et d'enseignement des géosciences de l'environnement (CEREGE), Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Collège de France (CdF (institution))-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA), Lamont-Doherty Earth Observatory (LDEO), University of Alberta, Collège de France (CdF (institution)), Unité de recherche Géosciences Marines (Ifremer) (GM), Institut des Sciences Chimiques de Rennes (ISCR), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA), School of Geography, Earth and Environmental Sciences [Birmingham], Dokuz Eylül Üniversitesi = Dokuz Eylül University [Izmir] (DEÜ), GeoForschungsZentrum - Helmholtz-Zentrum Potsdam (GFZ), Istituto di Science Marine (ISMAR ), Consiglio Nazionale delle Ricerche (CNR), Istituto Nazionale di Geofisica e Vulcanologia - Sezione di Roma (INGV), Institut de Physique du Globe de Paris (IPGP), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut national des sciences de l'Univers (INSU - CNRS)-IPG PARIS-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Centre National de la Recherche Scientifique (CNRS), EU [036851], MARSITE Integrated Project [308417], CNR, Ifremer, CNRS [MA201301A], Institut Carnot Ifremer-Edrome [06/11/2013], Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Collège de France (CdF)-Institut national des sciences de l'Univers (INSU - CNRS)-Aix Marseille Université (AMU)-Institut National de la Recherche Agronomique (INRA), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Institut National de la Recherche Agronomique (INRA)-Aix Marseille Université (AMU)-Collège de France (CdF (institution))-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Géosciences Marines (GM), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes (ENSCR)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Institut de Physique du Globe de Paris (IPG Paris)-Centre National de la Recherche Scientifique (CNRS), and ANR-16-CE03-0010,MAREGAMI2016,Caractérisation de la lacune sismique dans la région d'Istanbul(2016)
Subjects: [SDU.STU.TE]Sciences of the Universe [physics]/Earth Sciences/Tectonics, architecture, north anatolian fault, central basin, sea, [SDU.STU.GP]Sciences of the Universe [physics]/Earth Sciences/Geophysics [physics.geo-ph], Science, [SDU.STU]Sciences of the Universe [physics]/Earth Sciences, [ SDU.STU ] Sciences of the Universe [physics]/Earth Sciences, [ SDU.STU.TE ] Sciences of the Universe [physics]/Earth Sciences/Tectonics, trough, izmit earthquake, Article, [ SDU.STU.GP ] Sciences of the Universe [physics]/Earth Sciences/Geophysics [physics.geo-ph], main marmara fault, evolution, turkey, Medicine, NORTH ANATOLIAN FAULT, MAIN MARMARA FAULT, IZMIT EARTHQUAKE, CENTRAL BASIN, SEA, EVOLUTION, ARCHITECTURE, TURKEY, TROUGH, ZONE, zone, ComputingMilieux_MISCELLANEOUS
Abstract: International audience; Understanding micro-seismicity is a critical question for earthquake hazard assessment. Since the devastating earthquakes of Izmit and Duzce in 1999, the seismicity along the submerged section of North Anatolian Fault within the Sea of Marmara (comprising the "Istanbul seismic gap") has been extensively studied in order to infer its mechanical behaviour (creeping vs locked). So far, the seismicity has been interpreted only in terms of being tectonic-driven, although the Main Marmara Fault (MMF) is known to strike across multiple hydrocarbon gas sources. Here, we show that a large number of the aftershocks that followed the M 5.1 earthquake of July, 25th 2011 in the western Sea of Marmara, occurred within a zone of gas overpressuring in the 1.5-5 km depth range, from where pressurized gas is expected to migrate along the MMF, up to the surface sediment layers. Hence, gas-related processes should also be considered for a complete interpretation of the micro-seismicity (similar to M < 3) within the Istanbul offshore domain.
Published: 2018
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49. Carboxythiazole is a key microbial nutrient currency and critical component of thiamin biosynthesis

Author: Phillippe Schatt, Thomas D. Niehaus, Elden E. Rowland, Andrew D. Hanson, Mohamed Mehiri, Lasse Riemann, Ryan W. Paerl, Erin M. Bertrand, François-Yves Bouget, Department of Marine, Earth, and Atmospheric Sciences [NCSU] (MEAS), North Carolina State University [Raleigh] (NC State), University of North Carolina System (UNC)-University of North Carolina System (UNC), Department of Biology [Copenhagen], Faculty of Science [Copenhagen], University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU), Department of Biology (Dalhousie University), Dalhousie University [Halifax], Laboratoire d'Océanographie Microbienne (LOMIC), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire océanologique de Banyuls (OOB), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de Chimie de Nice (ICN), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Horticultural Sciences Department, University of Florida [Gainesville], ANR: 15-IDEX-0001,UCA JEDI,Idex UCA JEDI(2015), ANR-14-CE02-0018,Photo-Phyto,Effets du réchauffement climatique sur le déclenchement des blooms phytoplanctoniques marins : photoperiodisme, composition et adaptation(2014), European Project: 654008,H2020,H2020-INFRADEV-1-2014-1,EMBRIC(2015), University of North Carolina System (UNC), University of Copenhagen = Københavns Universitet (KU), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), University of Florida [Gainesville] (UF), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA)-Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)
Subjects: 0106 biological sciences, 0301 basic medicine, Water microbiology, Microorganism, Arabidopsis, lcsh:Medicine, Chemical ecology, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, Biology, medicine.disease_cause, 01 natural sciences, Article, Cell Line, Microbial ecology, 03 medical and health sciences, Thiamine biosynthesis, chemistry.chemical_compound, Mice, Nutrient, Biosynthesis, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, medicine, Escherichia coli, Arabidopsis thaliana, Animals, Thiamine, [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM], lcsh:Science, Primary productivity, [SDV.EE]Life Sciences [q-bio]/Ecology, environment, [SDU.OCEAN]Sciences of the Universe [physics]/Ocean, Atmosphere, Multidisciplinary, Arabidopsis Proteins, lcsh:R, Nutrients, biology.organism_classification, Thiazoles, 030104 developmental biology, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Biochemistry, chemistry, Phytoplankton, lcsh:Q, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, 010606 plant biology & botany
Abstract: Almost all cells require thiamin, vitamin B1 (B1), which is synthesized via the coupling of thiazole and pyrimidine precursors. Here we demonstrate that 5-(2-hydroxyethyl)-4-methyl-1,3-thiazole-2-carboxylic acid (cHET) is a useful in vivo B1 precursor for representatives of ubiquitous marine picoeukaryotic phytoplankton and Escherichia coli – drawing attention to cHET as a valuable exogenous micronutrient for microorganisms with ecological, industrial, and biomedical value. Comparative utilization experiments with the terrestrial plant Arabidopsis thaliana revealed that it can also use exogenous cHET, but notably, picoeukaryotic marine phytoplankton and E. coli were adapted to grow on low (picomolar) concentrations of exogenous cHET. Our results call for the modification of the conventional B1 biosynthesis model to incorporate cHET as a key precursor for B1 biosynthesis in two domains of life, and for consideration of cHET as a microbial micronutrient currency modulating marine primary productivity and community interactions in human gut-hosted microbiomes.
Published: 2018
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50. Interaction of Complement Defence Collagens C1q and Mannose-Binding Lectin with BMP-1/Tolloid-like Proteinases

Author: Nicole M. Thielens, Chantal Dumestre-Pérard, Monique Lacroix, Agnès Tessier, Sandrine Vadon-Le Goff, Catherine Moali, Sylvie Ricard-Blum, Leena Bruckner-Tuderman, Alexander Nyström, Dimitra Kiritsi, David J.S. Hulmes, Evelyne Gout, Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biologie Tissulaire et d'ingénierie Thérapeutique UMR 5305 (LBTI), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Immunologie, CHU Grenoble, Freiburg Institute for Advanced Studies, Albert-Ludwigs-Universität Freiburg, Universitätsklinikum Freiburg, Assemblages Supramoléculaires Péricellulaires et Extracellulaires (ASPE), Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), SPR (ISBG -UMS 3518), ANR-09-PIRI-0021,STR-ASS-DEF-COL(2009), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Proteases, Skin Neoplasms, Tolloid-Like Metalloproteinases, lcsh:Medicine, chemical and pharmacologic phenomena, Mannose-Binding Lectin, Bone morphogenetic protein 1, Article, Bone Morphogenetic Protein 1, 03 medical and health sciences, 0302 clinical medicine, Humans, lcsh:Science, Complement C1q, Complement Activation, Melanoma, Mannan-binding lectin, Multidisciplinary, Innate immune system, Binding Sites, [SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM], Chemistry, Extracellular matrix assembly, lcsh:R, Complement system, Cell biology, Epidermolysis Bullosa Dystrophica, 030104 developmental biology, lcsh:Q, Ficolin, 030215 immunology
Abstract: The defence collagens C1q and mannose-binding lectin (MBL) are immune recognition proteins that associate with the serine proteinases C1r/C1s and MBL-associated serine proteases (MASPs) to trigger activation of complement, a major innate immune system. Bone morphogenetic protein-1 (BMP-1)/tolloid-like proteinases (BTPs) are metalloproteinases with major roles in extracellular matrix assembly and growth factor signalling. Despite their different functions, C1r/C1s/MASPs and BTPs share structural similarities, including a specific CUB-EGF-CUB domain arrangement found only in these enzymes that mediates interactions with collagen-like proteins, suggesting a possible functional relationship. Here we investigated the potential interactions between the defence collagens C1q and MBL and the BTPs BMP-1 and mammalian tolloid-like-1 (mTLL-1). C1q and MBL bound to immobilized BMP-1 and mTLL-1 with nanomolar affinities. These interactions involved the collagen-like regions of the defence collagens and were inhibited by pre-incubation of C1q or MBL with their cognate complement proteinases. Soluble BMP-1 and mTLL-1 did not inhibit complement activation and the defence collagens were neither substrates nor inhibitors of BMP-1. Finally, C1q co-localized with BMP-1 in skin biopsies following melanoma excision and from patients with recessive dystrophic epidermolysis bullosa. The observed interactions provide support for a functional link between complement and BTPs during inflammation and tissue repair.
Published: 2017
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