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91 results on '"Holm P"'

1. Activation of glial cells induces proinflammatory properties in brain capillary endothelial cells in vitro

Author

Annette Burkhart, Steinunn Sara Helgudóttir, Yahye A. Mahamed, Mikkel B. Fruergaard, Julie N. Holm-Jacobsen, Hulda Haraldsdóttir, Sara E. Dahl, Freja Pretzmann, Lisa Greve Routhe, Kate Lambertsen, Torben Moos, and Maj Schneider Thomsen

Subjects
Blood-brain barrier, Neuroinflammation, In-vitro BBB model, Glial cells, Lipopolysaccharide, Medicine, Science
Abstract

Abstract Neurodegenerative diseases are often accompanied by neuroinflammation and impairment of the blood-brain barrier (BBB) mediated by activated glial cells through their release of proinflammatory molecules. To study the effects of glial cells on mouse brain endothelial cells (mBECs), we developed an in vitro BBB model with inflammation by preactivating mixed glial cells (MGCs) with lipopolysaccharide (LPS) before co-culturing with mBECs to study the influence of molecules released by activated MGCs. The response of the mBECs to activated MGCs was compared to direct stimulation with LPS. The cytokine profile of activated MGCs was analyzed together with their effects on the mBEC’s integrity, expression of tight junction proteins, adhesion molecules, and BBB-specific transport proteins. Stimulation of MGCs significantly upregulated mRNA expression and secretion of several pro-inflammatory cytokines. Co-culturing mBECs with pre-stimulated MGCs significantly affected the barrier integrity of mBECs similar to direct stimulation with LPS. The gene expression levels of tight junction proteins were unaltered, but tight junction proteins revealed rearrangements with respect to subcellular distribution. Compared to direct stimulation with LPS, the expression of cell-adhesion molecules was significantly increased when mBECs were co-cultured with prestimulated MGCs and thus pre-activating MGCs transforms mBECs into a proinflammatory phenotype.

Published
2024
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7. Genetic predictors of blood pressure traits are associated with preeclampsia

Author

Elizabeth A. Jasper, Jacklyn N. Hellwege, Joseph H. Breeyear, Brenda Xiao, Gail P. Jarvik, Ian B. Stanaway, Kathleen A. Leppig, Geetha Chittoor, M. Geoffrey Hayes, Ozan Dikilitas, Iftikhar J. Kullo, Ingrid A. Holm, Shefali Setia Verma, Todd L. Edwards, and Digna R. Velez Edwards

Subjects
Medicine, Science
Abstract

Abstract Preeclampsia, a pregnancy complication characterized by hypertension after 20 gestational weeks, is a major cause of maternal and neonatal morbidity and mortality. Mechanisms leading to preeclampsia are unclear; however, there is evidence of high heritability. We evaluated the association of polygenic scores (PGS) for blood pressure traits and preeclampsia to assess whether there is shared genetic architecture. Non-Hispanic Black and White reproductive age females with pregnancy indications and genotypes were obtained from Vanderbilt University’s BioVU, Electronic Medical Records and Genomics network, and Penn Medicine Biobank. Preeclampsia was defined by ICD codes. Summary statistics for diastolic blood pressure (DBP), systolic blood pressure (SBP), and pulse pressure (PP) PGS were acquired from Giri et al. Associations between preeclampsia and each PGS were evaluated separately by race and data source before subsequent meta-analysis. Ten-fold cross validation was used for prediction modeling. In 3504 Black and 5009 White included individuals, the rate of preeclampsia was 15.49%. In cross-ancestry meta-analysis, all PGSs were associated with preeclampsia (ORDBP = 1.10, 95% CI 1.02–1.17, p = 7.68 × 10−3; ORSBP = 1.16, 95% CI 1.09–1.23, p = 2.23 × 10−6; ORPP = 1.14, 95% CI 1.07–1.27, p = 9.86 × 10−5). Addition of PGSs to clinical prediction models did not improve predictive performance. Genetic factors contributing to blood pressure regulation in the general population also predispose to preeclampsia.

Published
2024
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8. Circulating C-reactive protein levels as a prognostic biomarker in breast cancer across body mass index groups

Author

J. B. Holm, E. Baggesen, D. Cronin-Fenton, J. Frystyk, J. M. Bruun, P. Christiansen, and S. Borgquist

Subjects
Breast cancer, Obesity, Body mass index, Inflammation, C-reactive protein, Prognosis, Medicine, Science
Abstract

Abstract Obesity and systemic inflammation are associated with breast cancer (BC) outcomes. Systemic inflammation is increased in obesity. We examined the association between C-reactive protein (CRP) and disease-free survival (DFS) and overall survival (OS) overall, and according to body mass index (BMI). We assembled a cohort of women with BC (stage I–III) seen at Aarhus University Hospital between 2010 and 2020 who donated blood at BC diagnosis (N = 2673). CRP levels were measured and divided into quartiles. We followed patients from surgery to recurrence, contralateral BC, other malignancy, death, emigration, or end-of-follow-up. We used Cox regression to estimate hazard ratios (HRs) with 95% confidence intervals (95% CIs) to compare outcomes across CRP quartiles, overall and stratified by BMI (normal-weight (18.5 ≤ BMI

Published
2024
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10. Novel environmental monitoring detector for discriminating fallout and airborne radioactivity

Author

Philip Holm, Sakari Ihantola, Ville Bogdanoff, Kari Peräjärvi, Peter Dendooven, Olof Tengblad, and Maarit Muikku

Subjects
Medicine, Science
Abstract

Abstract Early warning networks are used for detecting abnormal radioactivity levels in the environment. State-of-the-art networks are equipped with both dose rate detectors and spectrometric stations. Current networks don’t automatically discriminate between radioactivity on the ground and in the air. A novel directional sensing gamma radiation detector utilizing a collimated phoswich scintillator was developed. The signals from the two scintillator materials are separated using a pulse shape discrimination. The separated signals are employed to determine the radioactivity concentrations on the ground and in the air assuming specific concentration distributions. Limitations related to imperfect directional sensing and dead time are discussed.

Published
2023
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11. Regionalization of cell types in silk glands of Larinioides sclopetarius suggest that spider silk fibers are complex layered structures

Author

Sumalata Sonavane, Per Westermark, Anna Rising, and Lena Holm

Subjects
Medicine, Science
Abstract

Abstract In order to produce artificial silk fibers with properties that match the native spider silk we likely need to closely mimic the spinning process as well as fiber architecture and composition. To increase our understanding of the structure and function of the different silk glands of the orb weaver Larinioides sclopetarius, we used resin sections for detailed morphology, paraffin embedded sections for a variety of different histological stainings, and a histochemical method for localization of carbonic anhydrase activity. Our results show that all silk glands, except the tubuliform glands, are composed of two or more columnar epithelial cell types, some of which have not been described previously. We observed distinct regionalization of the cell types indicating sequential addition of secretory products during silk formation. This means that the major ampullate, minor ampullate, aciniform type II, and piriform silk fibers most likely are layered and that each layer has a specific composition. Furthermore, a substance that stains positive for polysaccharides may be added to the silk in all glands except in the type I aciniform glands. Active carbonic anhydrase was found in all silk glands and/or ducts except in the type I aciniform and tubuliform glands, with the strongest staining in aggregate glands and their ductal nodules. Carbonic anhydrase plays an important role in the generation of a pH gradient in the major ampullate glands, and our results suggest that some other glands may also harbor pH gradients.

Published
2023
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12. A novel biomarker of MMP-cleaved cartilage intermediate layer protein-1 is elevated in patients with rheumatoid arthritis, ankylosing spondylitis and osteoarthritis

Author

Helena Port, Cecilie Møller Hausgaard, Yi He, Walter P. Maksymowych, Stephanie Wichuk, Dovile Sinkeviciute, Anne-Christine Bay-Jensen, and Signe Holm Nielsen

Subjects
Medicine, Science
Abstract

Abstract Rheumatic joints have an altered cartilage turnover. Cartilage intermediate layer protein 1 (CILP-1) is secreted from articular chondrocytes and deposited into the cartilage extracellular matrix. We developed an immunoassay targeting a Matrix Metalloproteinase (MMP)-generated neo-epitope of CILP-1, named CILP-M. Human articular cartilage was cleaved with proteolytic enzymes and CILP-M levels were measured. We also quantified CILP-M in two studies from patients with rheumatoid arthritis (RA), ankylosing spondylitis (AS) and osteoarthritis (OA) and explored the monitoring and prognostic potential of CILP-M in TNF-α inhibitory treatment and modified Stoke AS Spine Score (mSASSS) progression. CILP-M was generated by MMP-1, -8 and -12. In the discovery study, CILP-M was significantly higher in patients with RA, AS and OA than healthy donors (p 0.95 (p 0.90 (p

Published
2023
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33. SARS-CoV-2 detection using reverse transcription strand invasion based amplification and a portable compact size instrument

Author

Rosenstierne, Maiken W., Joshi, Shreya, Danielsen, E. Thomas, Webb, Helen, Luong, Dac Mui, Bjerring, Julie, Hindkær, Julie, Jørgensen, Lærke, Blauenfeldt, Julie, Bojesen, Ask, Holck, Flemming, Lau, Johnny Weber, Bangsgaard, Lars, Lind, Jakob Broberg, Dragheim, Mette Bjergaard, Jacobsen, Mikkel Rohde, Elkjær, Robert, Clauwaert, Steven, Christensen, Kristina, Polacek, Charlotta, Fomsgaard, Anders, Ojalehto, Tuomas, Tullila, Antti, Brummer, Mirko, Jensen, Claus Juel, Jensen, Frederikke Holm, Schneider, Uffe Vest, Lisby, Jan Gorm, Jørgensen, Rikke Lind, Warthoe, Thomas, Finding, Ebbe, and Warthoe, Peter

Published
2021
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38. Publisher Correction: Automated application of low energy electron irradiation enables inactivation of pathogen- and cell-containing liquids in biomedical research and production facilities

Author

Fertey, Jasmin, Thoma, Martin, Beckmann, Jana, Bayer, Lea, Finkensieper, Julia, Reißhauer, Susann, Berneck, Beatrice Sarah, Issmail, Leila, Schönfelder, Jessy, Casado, Javier Portillo, Poremba, Andre, Rögner, Frank-Holm, Standfest, Bastian, Makert, Gustavo R., Walcher, Lia, Kistenmacher, Ann-Kathrin, Fricke, Stephan, Grunwald, Thomas, and Ulbert, Sebastian

Published
2020
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43. Automated application of low energy electron irradiation enables inactivation of pathogen- and cell-containing liquids in biomedical research and production facilities

Author

Fertey, Jasmin, Thoma, Martin, Beckmann, Jana, Bayer, Lea, Finkensieper, Julia, Reißhauer, Susann, Berneck, Beatrice Sarah, Issmail, Leila, Schönfelder, Jessy, Casado, Javier Portillo, Poremba, Andre, Rögner, Frank-Holm, Standfest, Bastian, Makert, Gustavo R., Walcher, Lia, Kistenmacher, Ann-Kathrin, Fricke, Stephan, Grunwald, Thomas, and Ulbert, Sebastian

Published
2020
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46. Rosuvastatin alters the genetic composition of the human gut microbiome

Author

Kummen, Martin, Solberg, Ole Geir, Storm-Larsen, Christopher, Holm, Kristian, Ragnarsson, Asgrimur, Trøseid, Marius, Vestad, Beate, Skårdal, Rita, Yndestad, Arne, Ueland, Thor, Svardal, Asbjørn, Berge, Rolf K., Seljeflot, Ingebjørg, Gullestad, Lars, Karlsen, Tom H., Aaberge, Lars, Aukrust, Pål, and Hov, Johannes R.

Published
2020
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