1. Respiratory mycobiome and suggestion of inter-kingdom network during acute pulmonary exacerbation in cystic fibrosis.
- Author
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Soret, Perrine, Vandenborght, Louise-Eva, Francis, Florence, Coron, Noémie, Enaud, Raphael, Avalos, Marta, Schaeverbeke, Thierry, Berger, Patrick, Fayon, Michael, Thiebaut, Rodolphe, Delhaes, Laurence, The Mucofong Investigation Group, Chabe, Magali, Audebert, Christophe, Durand-Joly, Isabelle, Boldron, Amale, Pin, Isabelle, Cognet, Odile, Pelloux, Herve, and Prevotat, Anne
- Subjects
LUNG infections ,MYCOBIOME ,DISEASE exacerbation ,CYSTIC fibrosis ,RIBOSOMAL RNA - Abstract
Lung infections play a critical role in cystic fibrosis (CF) pathogenesis. CF respiratory tract is now considered to be a polymicrobial niche and advances in high-throughput sequencing allowed to analyze its microbiota and mycobiota. However, no NGS studies until now have characterized both communities during CF pulmonary exacerbation (CFPE). Thirty-three sputa isolated from patients with and without CFPE were used for metagenomic high-throughput sequencing targeting 16S and ITS2 regions of bacterial and fungal rRNA. We built inter-kingdom network and adapted Phy-Lasso method to highlight correlations in compositional data. The decline in respiratory function was associated with a decrease in bacterial diversity. The inter-kingdom network revealed three main clusters organized around Aspergillus, Candida, and Scedosporium genera. Using Phy-Lasso method, we identified Aspergillus and Malassezia as relevantly associated with CFPE, and Scedosporium plus Pseudomonas with a decline in lung function. We corroborated in vitro the cross-domain interactions between Aspergillus and Streptococcus predicted by the correlation network. For the first time, we included documented mycobiome data into a version of the ecological Climax/Attack model that opens new lines of thoughts about the physiopathology of CF lung disease and future perspectives to improve its therapeutic management. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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