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8 results on '"Castiglioni, Sara"'

5. Increased levels of the oxidative stress biomarker 8-iso-prostaglandin F2α in wastewater associated with tobacco use

Author

Ryu, Yeonsuk, primary, Gracia-Lor, Emma, additional, Bade, Richard, additional, Baz-Lomba, J. A., additional, Bramness, Jørgen G., additional, Castiglioni, Sara, additional, Castrignanò, Erika, additional, Causanilles, Ana, additional, Covaci, Adrian, additional, de Voogt, Pim, additional, Hernandez, Felix, additional, Kasprzyk-Hordern, Barbara, additional, Kinyua, Juliet, additional, McCall, Ann-Kathrin, additional, Ort, Christoph, additional, Plósz, Benedek G., additional, Ramin, Pedram, additional, Rousis, Nikolaos I., additional, Reid, Malcolm J., additional, and Thomas, Kevin V., additional

Published
2016
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6. Increased levels of the oxidative stress biomarker 8-iso-prostaglandin F2α in wastewater associated with tobacco use.

Author

Ryu, Yeonsuk, Gracia-Lor, Emma, Bade, Richard, Baz-Lomba, J. A., Bramness, Jørgen G., Castiglioni, Sara, Castrignanò, Erika, Causanilles, Ana, Covaci, Adrian, de Voogt, Pim, Hernandez, Felix, Kasprzyk-Hordern, Barbara, Kinyua, Juliet, McCall, Ann-Kathrin, Ort, Christoph, Plósz, Benedek G., Ramin, Pedram, Rousis, Nikolaos I., Reid, Malcolm J., and Thomas, Kevin V.

Abstract

Wastewater analysis has been demonstrated to be a complementary approach for assessing the overall patterns of drug use by a population while the full potential of wastewater-based epidemiology has yet to be explored. F2-isoprostanes are a prototype wastewater biomarker to study the cumulative oxidative stress at a community level. In this work, 8-iso-prostaglandin F2α (8-iso-PGF2α) was analysed in raw 24 h-composite wastewater samples collected from 4 Norwegian and 7 other European cities in 2014 and 2015. Using the same samples, biomarkers of alcohol (ethyl sulfate) and tobacco (trans-3′-hydroxycotinine) use were also analysed to investigate any possible correlation between 8-iso-PGF2α and the consumption of the two drugs. The estimated per capita daily loads of 8-iso-PGF2α in the 11 cities ranged between 2.5 and 9.9 mg/day/1000 inhabitants with a population-weighted mean of 4.8 mg/day/1000 inhabitants. There were no temporal trends observed in the levels of 8-iso-PGF2α, however, spatial differences were found at the inter-city level correlating to the degree of urbanisation. The 8-iso-PGF2α mass load was found to be strongly associated with that of trans-3′-hydroxycotinine while it showed no correlation with ethyl sulfate. The present study shows the potential for 8-iso-PGF2α as a wastewater biomarker for the assessment of community public health. [ABSTRACT FROM AUTHOR]

Published
2016
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7. Magnesium homeostasis in colon carcinoma LoVo cells sensitive or resistant to doxorubicin

Author

Lucia Merolle, Giovanna Farruggia, Valentina Trapani, Stefano Iotti, Federica I. Wolf, Concettina Cappadone, Sara Castiglioni, Alessandra Cazzaniga, Jeanette A.M. Maier, Castiglioni, Sara, Cazzaniga, Alessandra, Trapani, Valentina, Cappadone, Concettina, Farruggia, Giovanna, Merolle, Lucia, Wolf, Federica I., Iotti, Stefano, and Maier, Jeanette A. M

Subjects
medicine.medical_specialty, Blotting, Western, Colon carcinoma, TRPM Cation Channels, chemistry.chemical_element, Cancer cell, Cysteine Proteinase Inhibitors, Protein Serine-Threonine Kinases, Biology, Article, multidrug resistance, Settore MED/04 - PATOLOGIA GENERALE, Cell Line, Tumor, Internal medicine, medicine, Homeostasis, Humans, Gene silencing, Magnesium, Doxorubicin, Antibiotics, Antineoplastic, Ion Transport, Multidisciplinary, Calpain, Reverse Transcriptase Polymerase Chain Reaction, ion channels, Dipeptides, Gene Expression Regulation, Neoplastic, Endocrinology, chemistry, Drug Resistance, Neoplasm, Cell culture, Colonic Neoplasms, Cancer research, biology.protein, RNA Interference, Intracellular, medicine.drug
Abstract

Neoplastic cells accumulate magnesium, an event which provides selective advantages and is frequently associated with TRPM7overexpression. Little is known about magnesium homeostasis in drug-resistant cancer cells. Therefore, we used the colon cancer LoVo cell model and compared doxorubicin-resistant to sensitive cells. In resistant cells the concentration of total magnesium is higher while its influx capacity is lower than in sensitive cells. Accordingly, resistant cells express lower amounts of the TRPM6 and 7, both involved in magnesium transport. While decreased TRPM6 levels are due to transcriptional regulation, post-transcriptional events are involved in reducing the amounts of TRPM7. Indeed, the calpain inhibitor calpeptin markedly increases the levels of TRPM7 in resistant cells. In doxorubicin-sensitive cells, silencing TRPM7 shifts the phenotype to one more similar to resistant cells, since in these cells silencing TRPM7 significantly decreases the influx of magnesium, increases its intracellular concentration and increases resistance to doxorubicin. On the other hand, calpain inhibition upregulates TRPM7, decreases intracellular magnesium and enhances the sensitivity to doxorubicin of resistant LoVo cells. We conclude that in LoVo cells drug resistance is associated with alteration of magnesium homeostasis through modulation of TRPM7. Our data suggest that TRPM7 expression may be an additional undisclosed player in chemoresistance.

Published
2015

8. Increased levels of the oxidative stress biomarker 8-iso-prostaglandin F 2α in wastewater associated with tobacco use.

Author

Ryu Y, Gracia-Lor E, Bade R, Baz-Lomba JA, Bramness JG, Castiglioni S, Castrignanò E, Causanilles A, Covaci A, de Voogt P, Hernandez F, Kasprzyk-Hordern B, Kinyua J, McCall AK, Ort C, Plósz BG, Ramin P, Rousis NI, Reid MJ, and Thomas KV

Subjects
Biomarkers analysis, Cities, Cotinine analysis, Dinoprost analysis, Europe, Humans, Norway, Oxidative Stress, Regression Analysis, Sulfuric Acid Esters analysis, Urbanization, Cotinine analogs & derivatives, Dinoprost analogs & derivatives, Tobacco Use epidemiology, Wastewater analysis
Abstract

Wastewater analysis has been demonstrated to be a complementary approach for assessing the overall patterns of drug use by a population while the full potential of wastewater-based epidemiology has yet to be explored. F 2 -isoprostanes are a prototype wastewater biomarker to study the cumulative oxidative stress at a community level. In this work, 8-iso-prostaglandin F 2α (8-iso-PGF 2α ) was analysed in raw 24 h-composite wastewater samples collected from 4 Norwegian and 7 other European cities in 2014 and 2015. Using the same samples, biomarkers of alcohol (ethyl sulfate) and tobacco (trans-3'-hydroxycotinine) use were also analysed to investigate any possible correlation between 8-iso-PGF 2α and the consumption of the two drugs. The estimated per capita daily loads of 8-iso-PGF 2α in the 11 cities ranged between 2.5 and 9.9 mg/day/1000 inhabitants with a population-weighted mean of 4.8 mg/day/1000 inhabitants. There were no temporal trends observed in the levels of 8-iso-PGF 2α , however, spatial differences were found at the inter-city level correlating to the degree of urbanisation. The 8-iso-PGF 2α mass load was found to be strongly associated with that of trans-3'-hydroxycotinine while it showed no correlation with ethyl sulfate. The present study shows the potential for 8-iso-PGF 2α as a wastewater biomarker for the assessment of community public health.

Published
2016
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