1. Transport of Live Cells Under Sterile Conditions Using a Chemotactic Droplet
- Author
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Martin M. Hanczyc, Carlotta Porcelli, Ioannis Ieropoulos, and Silvia Holler
- Subjects
lcsh:Medicine ,Saccharomyces cerevisiae ,02 engineering and technology ,Bacillus subtilis ,010402 general chemistry ,complex mixtures ,01 natural sciences ,Article ,lcsh:Science ,Electrochemical gradient ,Multidisciplinary ,Aqueous solution ,Bacteria ,Aqueous medium ,biology ,Chemistry ,Chemotaxis ,lcsh:R ,fungi ,technology, industry, and agriculture ,Bristol Bio-Energy Centre ,Capsule ,Biological Transport ,live cells, chemotactic droplet, escherichia coli, bacillus subtilis, sterile conditions, soft matter systems, bioengineering, chemotaxis ,021001 nanoscience & nanotechnology ,biology.organism_classification ,eye diseases ,0104 chemical sciences ,Biophysics ,lcsh:Q ,Fatty Alcohols ,0210 nano-technology ,Hydrophobic and Hydrophilic Interactions - Abstract
1-Decanol droplets, formed in an aqueous medium containing decanoate at high pH, become chemotactic when a chemical gradient is placed in the external aqueous environment. We investigated if such droplets can be used as transporters for living cells. We developed a partially hydrophobic alginate capsule as a protective unit that can be precisely placed in a droplet and transported along chemical gradients. Once the droplets with cargo reached a defined final destination, the association of the alginate capsule and decanol droplet was disrupted and cargo deposited. Both Escherichia coli and Bacillus subtilis cells survived and proliferated after transport even though transport occurred under harsh and sterile conditions.
- Published
- 2018
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