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Your search keyword '"Wen, Shu"' showing total 2 results
Start Over Author "Wen, Shu" Topic gene targeting Journal scientific reports
2 results on '"Wen, Shu"'

1. A multicolor panel of TALE-KRAB based transcriptional repressor vectors enabling knockdown of multiple gene targets.

Author

Zhang Z, Wu E, Qian Z, and Wu WS

Subjects
Color, HEK293 Cells, Humans, Gene Knockdown Techniques methods, Gene Targeting methods, Genetic Vectors genetics, Lentivirus genetics, Repressor Proteins genetics, Transcription, Genetic genetics
Abstract

Stable and efficient knockdown of multiple gene targets is highly desirable for dissection of molecular pathways. Because it allows sequence-specific DNA binding, transcription activator-like effector (TALE) offers a new genetic perturbation technique that allows for gene-specific repression. Here, we constructed a multicolor lentiviral TALE-Kruppel-associated box (KRAB) expression vector platform that enables knockdown of multiple gene targets. This platform is fully compatible with the Golden Gate TALEN and TAL Effector Kit 2.0, a widely used and efficient method for TALE assembly. We showed that this multicolor TALE-KRAB vector system when combined together with bone marrow transplantation could quickly knock down c-kit and PU.1 genes in hematopoietic stem and progenitor cells of recipient mice. Furthermore, our data demonstrated that this platform simultaneously knocked down both c-Kit and PU.1 genes in the same primary cell populations. Together, our results suggest that this multicolor TALE-KRAB vector platform is a promising and versatile tool for knockdown of multiple gene targets and could greatly facilitate dissection of molecular pathways.

Published
2014
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2. A multicolor panel of TALE-KRAB based transcriptional repressor vectors enabling knockdown of multiple gene targets

Author

Zhonghui Zhang, Wen Shu Wu, Zhijian Qian, and Elise Wu

Subjects
Genetics, Transcription activator-like effector nuclease, Gene knockdown, Multidisciplinary, Expression vector, Transcription, Genetic, Effector, HEK 293 cells, Genetic Vectors, Lentivirus, Color, Biology, Article, Cell biology, Repressor Proteins, HEK293 Cells, TAL effector, Transcription (biology), Gene Knockdown Techniques, Gene Targeting, Humans, Gene
Abstract

Stable and efficient knockdown of multiple gene targets is highly desirable for dissection of molecular pathways. Because it allows sequence-specific DNA binding, transcription activator-like effector (TALE) offers a new genetic perturbation technique that allows for gene-specific repression. Here, we constructed a multicolor lentiviral TALE-Kruppel-associated box (KRAB) expression vector platform that enables knockdown of multiple gene targets. This platform is fully compatible with the Golden Gate TALEN and TAL Effector Kit 2.0, a widely used and efficient method for TALE assembly. We showed that this multicolor TALE-KRAB vector system when combined together with bone marrow transplantation could quickly knock down c-kit and PU.1 genes in hematopoietic stem and progenitor cells of recipient mice. Furthermore, our data demonstrated that this platform simultaneously knocked down both c-Kit and PU.1 genes in the same primary cell populations. Together, our results suggest that this multicolor TALE-KRAB vector platform is a promising and versatile tool for knockdown of multiple gene targets and could greatly facilitate dissection of molecular pathways.

Published
2014

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