1. Bladder outlet obstruction disrupts circadian bladder function in mice.
- Author
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Kitta, Takeya, Chiba, Hiroki, Kanno, Yukiko, Hattori, Tsuyoshi, Higuchi, Madoka, Ouchi, Mifuka, Togo, Mio, Takahashi, Yui, Michishita, Mai, Kitano, Tatsuya, and Shinohara, Nobuo
- Subjects
CIRCADIAN rhythms ,URINATION ,BLADDER obstruction ,BODY weight ,MESSENGER RNA ,SEROTONINERGIC mechanisms - Abstract
The circadian clock programs daily rhythms and coordinates multiple behavioural processes, including micturition. Partial bladder outlet obstruction (pBOO) in mice produces hyperactive voiding. However, long-term effects of pBOO on bladder function have not been clarified. In this study, we investigated micturition under conditions of impaired circadian bladder function by inducing long-term pBOO by tying the proximal urethra. Micturition behavior was evaluated at 1, 3, 6 and 12 months after surgery. We used automated voided stain on paper method for a precise micturition recording for mice. And quantitative assessment of gene expression was performed at 24 months after pBOO surgery using qRT-PCR procedure. The micturition frequencies in the pBOO group were significantly decreased at 3, 6, and 12 months compared to those at 1 month after operation in the same group (p < 0.05). Body weight of pBOO mice was significantly increased compared to sham operated mice at 12 months. The expression level of mRNA was exhibited a 3.4-fold nominal increased for a 5-HT2B receptor in the pBOO group compared to the sham group. The current study found that long-term pBOO led to disruption of the circadian bladder function (the day/night cycle) in mice, similar to those observed in human as nocturia. This disruption is possible involvement of the gain of body weight and/or serotonergic alteration after pBOO. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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