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In the rhizospheres of three gypsophytes and in non-rhizospheric soil, two samplings were carried out - the first during a summer drought and the second during spring - to detect the responses to the availability of water in the soil. Urease and protease showed higher values after the drought whereas β-glucosidase was highest in the spring. This pattern was the same for all the rhizospheres tested. However, the arylsulfatase and alkaline phosphatase did not change. Surprising results were obtained when water retention and water loss were studied, with the highest values being obtained for the dry season due to the association of Cyanoprokaryota with the rhizospheres. The results are also explained by two water pulses that occurred before the samplings. Several parameters, whose values changed markedly due to the microbiological activation just after the drought and water pulses, are proposed as indicators of this activation: microbial biomass carbon and basal respiration rate, together with urease and protease. However, it was the dehydrogenase activity in spring that best reflected the microbiology associated with the carbon cycle, together with β-glucosidase. The interrelationships between carbon and nitrogen were shown through the indices: water soluble nitrogen and water soluble carbon. We propose three functional adaptation mechanisms of these plants associated with the Cyanoprokaryota in their rhizospheres and related to the water availability as determined by drought and water pulse effects. Herniaria fruticosa is a pioneer with the greatest diversity of Cyanoprokaryota, in both summer and spring (10 species and 11 species, respectively), and with high-medium abundance (5–30%). Teucrium balthazaris exhibits an intermediate strategy, with greater diversity of Cyanoprokaryota in spring (7 species) and predominance of high-medium abundance (5–30%). Finally, Helianthemum squamatum has lower diversity, with one species in summer (with low abundance, <5%) and no species in spring. Unlabelled Image • Cyanoprokaryota had a relevant role in the rhizospheres of gypsophytes in drought conditions. • Three types of adaptation mechanisms of the gypsophytes were observed, depending of the cyanoprokaryota. • Gypsiferous communities were activated by pulses of water. • Under drought the nitrogen cycle was activated and in spring the carbon cycle. [ABSTRACT FROM AUTHOR]

Abstract Cyanobacteria are an important component of microalgae communities in aquatic ecosystems, however, their response to environmental factors in different habitats and catchment areas of small water bodies is still not well recognised. We examined ponds from two types of surroundings (field vs. forest) in order to find the best triggers for the distribution of cyanobacteria species, and analysed different habitats (open water and macrophyte-dominated zones) to find the habitat preferences of cyanobacteria species. Our results underline the important role of habitats in the determination of the abundance of cyanobacteria species in small water bodies. Cyanobacteria as a group preferred macrophyte-dominated sites with stable water column conditions, which to lesser extent were inhabited by representatives of other systematic groups of algae. The co-occurrence of many cyanobacteria species and zooplankton in the studied ponds may have indirectly resulted from biotic interactions in the food web. In the open water a positive relationship between zooplankton and cyanobacteria suggests stimulation of their development through the elimination of smaller edible taxa or by nutrient resupply through zooplankton excretion. The type of catchment area also impacted the cyanobacterial community. Field ponds with significantly higher values of pH and NO 3 were characterised by a higher abundance of cyanobacteria compared with ponds within the forest catchment. A positive relationship between pH and cyanobacteria indicates that they raise pH during photosynthesis. However, some species were negatively associated with water temperature and occurred exclusively only in forest ponds. The study revealed that cyanobacteria in small water bodies can be a valuable indicator of important ecosystem conditions. Despite the fact that their prevalence in agricultural ponds may confirm their potential as an indicator of pollution, their high diversity associated with macrophytes contributes to an increase of overall landscape biodiversity. Graphical abstract Unlabelled Image Highlights • Cyanobacteria, unlike other algae, responded differently to environmental factors. • In small water bodies cyanobacteria were associated with pH, NO 3 and NH 4. • Pond cyanobacteria preferred macrophyte-dominated sites compared to open water. • Field ponds had higher cyanobacteria density and lower diversity than forest ponds. • Filamentous forms dominated, particularly in field ponds and among macrophytes. [ABSTRACT FROM AUTHOR]

Abstract: Cyanobacterial toxins constitute one of the most high risk categories of waterborne toxic biological substances. For this reason there is a clear need to know which freshwater environments are most susceptible to the development of large populations of cyanobacteria. Phytoplankton data from 134 UK lakes were used to develop a series of Generalised Additive Models and Generalised Additive Mixed Models to describe which kinds of lakes may be susceptible to cyanobacterial blooms using widely available explanatory variables. Models were developed for log cyanobacterial biovolume. Water colour and alkalinity are significant explanatory variables and retention time and TP borderline significant (R2-adj=21.9%). Surprisingly, the models developed reveal that nutrient concentrations are not the primary explanatory variable; water colour and alkalinity were more important. However, given suitable environments (low colour, neutral-alkaline waters), cyanobacteria do increase with both increasing retention time and increasing TP concentrations, supporting the observations that cyanobacteria are one of the most visible symptoms of eutrophication, particularly in warm, dry summers. The models can contribute to the assessment of risks to public health, at a regional- to national level, helping target lake monitoring and management more cost-effectively at those lakes at the highest risk of breaching World Health Organisation guideline levels for cyanobacteria in recreational waters. The models also inform restoration options available for reducing cyanobacterial blooms, indicating that, in the highest risk lakes (alkaline, low colour lakes), risks can generally be lessened through management aimed at reducing nutrient loads and increasing flushing during summer. [Copyright &y& Elsevier]

Microcystis spp., are Gram-negative, oxygenic, photosynthetic prokaryotes which use solar energy to convert carbon dioxide (CO 2) and minerals into organic compounds and biomass. Eutrophication, rising CO 2 concentrations and global warming are increasing Microcystis blooms globally. Due to its high availability and protein content, Microcystis biomass has been suggested as a protein source for animal feeds. This would reduce dependency on soybean and other agricultural crops and could make use of "waste" biomass when Microcystis scums and blooms are harvested. Besides proteins, Microcystis contain further nutrients including lipids, carbohydrates, vitamins and minerals. However, Microcystis produce cyanobacterial toxins, including microcystins (MCs) and other bioactive metabolites, which present health hazards. In this review, challenges of using Microcystis blooms in feeds are identified. First, nutritional and toxicological (nutri-toxicogical) data, including toxicity of Microcystis to mollusks, crustaceans, fish, amphibians, mammals and birds, is reviewed. Inclusion of Microcystis in diets caused greater mortality, lesser growth, cachexia, histopathological changes and oxidative stress in liver, kidney, gill, intestine and spleen of several fish species. Estimated daily intake (EDI) of MCs in muscle of fish fed Microcystis might exceed the provisional tolerable daily intake (TDI) for humans, 0.04 μg/kg body mass (bm)/day, as established by the World Health Organization (WHO), and is thus not safe. Muscle of fish fed M. aeruginosa is of low nutritional value and exhibits poor palatability/taste. Microcystis also causes hepatotoxicity, reproductive toxicity, cardiotoxicity, neurotoxicity and immunotoxicity to mollusks, crustaceans, amphibians, mammals and birds. Microbial pathogens can also occur in blooms of Micr ocystis. Thus, cyanotoxins/xenobiotics/pathogens in Microcystis biomass should be removed/degraded/inactivated sufficiently to assure safety for use of the biomass as a primary/main/supplemental ingredient in animal feed. As an ameliorative measure, antidotes/detoxicants can be used to avoid/reduce the toxic effects. Before using Microcystis in feed ingredients/supplements, further screening for health protection and cost control is required. Unlabelled Image • Challenges of using blooms of Microcystis spp. in animal feeds were reviewed. • Microcystis causes toxicity to mollusks, crustaceans, fish, amphibians, mammals and birds. • Microcystis induces toxicity in liver, kidney, intestine, spleen and other organs. • Fish fed Microcystis may be not safe for consumption for humans. • Microbial pathogens may be present in cyanobacterial blooms. [ABSTRACT FROM AUTHOR]

A high incidence of harmful algal bloom in eutrophic surface waters causes many environmental problems. Thermophilic aerobic composting enables effective treatment and disposal of algal sludge that remains after the dewatering of algae slurries, and provides a value-added organic fertiliser. Previous studies have either only dealt with the composting of a single waste component or were conducted at a lab-/pilot-scale; however, this work is a comprehensive assessment of full-scale mechanized thermophilic aerobic co-composting of algal sludge and other typical biomass-based wastes, including chicken faeces and rice straw, in a water-rich rural area in the Tai lake basin, China. With the optimised feedstock material mass ratio (6.0:1.8:1.0 for straw:algae:faeces; initial C/N ratio of 20; and initial moisture of 60 wt%), the co-composting process effectively achieved the reduction, harmlessness, and reuse of waste. The moisture content (28.36 wt% of wet weight), organic matter content (57.91 wt% of dried weight), total nutrient content (6.59 wt% for TN + TP + TK of dried weight), and heavy metal contents as well as the pH of the final product fully met the Chinese National Agricultural Organic Fertiliser Standard requirements. The reduction rates of microcystin and toxic volatile fatty acid contents were higher than 99.5%, and the seed germination index of the product was 114.5%. A notable economic benefit with a gross profit margin of 167–434% of the process was highlighted. Investigation of the associated mechanisms, including statistical analysis, spectral characterisation, micro-morphological observation, and microbial community analysis, revealed that a decreased particle sizes with a looser structure and an efficient humification effect, resulting from the work of several identified dominant microbial species, contributed to the high product quality. The current study provided a demonstration of the promising full-scale co-composting technology for comprehensive management of the environment in water-rich rural areas and the construction of a sustainable watershed. Unlabelled Image • Full-scale mechanized composting of algae with chicken faeces and straw is assessed. • Compost product quality fully meets the requirement of organic fertiliser standard. • Toxic microcystin and volatile fatty acid contents are decreased by >99.5%. • Notable economic benefit with a gross profit margin of 167%–434% is calculated. • Dominant aerobic thermophilic microbial species for composting are identified. [ABSTRACT FROM AUTHOR]

Degradation of lake conditions could result from many stressors generated by human disturbance. Accurately defining lake ecological condition by multimetric index (MMI) method is of great importance for tracking source of stressors and lake management. For algal assessment, seldom have structural and functional attributes of soft-bodied planktonic algae metrics, one important dimension of biological condition, been used to develop MMI in conjunction with diatom metrics. Another thing is that some researchers found MMI method does not perform well in mid- and high-disturbed lakes. To test the aforementioned questions, we used data sets of the 2007 National Lake Assessment project of the USEPA to develop MMIs with and without using soft-bodied planktonic algae metrics for plains and lowlands area (PLNLOW, high disturbed region of the US) and across the conterminous US. Compared to site groups modeled by single diatom assemblages, we found integrating soft-bodied planktonic algae metric (especially blue-green algae metric) into developing MMIs can significantly improve performance of MMI in PLNLOW region. The separation powers of MMIs of five level III ecoregions, developed by incorporating blue-green algae metric, are consistently higher than those developed by single diatom assemblages (p-value = 0.029). However, when blue-green algae metric was applied to develop MMI along with diatom metrics in the national scale assessment, performances of MMIs are similar to that developed by diatom metrics (0.14 < p-value < 0.86). Different performance of MMIs developed by integrating blue-green algae metric at different spatial scales indicated the usefulness of blue-green algae metric in ecological assessment in mid- and high- disturbed lakes and a tiered approach for using diatom and blue-green algae metric in ecological assessment. We suggest using blue-green algae metric in combined with diatom metric to develop MMI when lakes are mid- and high-disturbed, while a routine diatom assessment would be enough for minimally disturbed sites. Unlabelled Image • Diatom assemblage-based MMI did not perform well in degraded lakes. • MMI performance improved by incorporating blue-green algae metrics in degraded lakes. • A tiered approach is recommended for ecological assessment. [ABSTRACT FROM AUTHOR]