Biochar application is considered an effective method of reducing nitrous oxide (N 2 O) emissions in soil. However, the mechanism and temporal effect of different doses of biochar on N 2 O emissions is still obscure. Here, we conducted a two-year field experiment to test the effects of different input amounts and frequencies of biochar on soil N 2 O emissions in North China. Biochar was applied in six different treatments in a winter wheat and summer maize rotation system: applications of 0 t/ha biochar (C0), 2.25 t/ha biochar (C1), 4.5 t/ha biochar (C2), 9 t/ha biochar (C3), and 13.5 t/ha biochar (C4) each year, and a single application of 13.5 t/ha biochar (CS) in the first year. The results showed that biochar could inhibit N 2 O emissions, reaching 20.6% to 60.1% in the wheat season and 18.1% to 39.4% in the maize season. The inhibitory effect of biochar on soil N 2 O emissions was dependent on amount and time. C3 had the best results in the wheat season, although its inhibitory effect in the maize season was not as good relative to C4 due to the lower biochar application. In addition, CS significantly reduced (27.7%) the cumulative N 2 O emissions in the first year, although the inhibitory effect disappeared in the second year. Biochar increased the nosZ gene copy numbers and promoted a reduction of N 2 O in the soil via the denitrification process. In conclusion, the inhibition of N 2 O emissions during denitrification is an important factor for reducing soil N 2 O emissions by biochar, and the inhibition of biochar is influenced by the input amount and time., Competing Interests: Declaration of competing interest Hongyuan liu: Conceptualization, Methodology, Writing-Original Draft, Formal analysis. Hongbo li: Data curation, Writing-Review & Editing. Aiping Zhang: Writing-Review & Editing, Supervision. Md Arifur Rahaman: Writing-Review & Editing. Zhengli Yang: Supervision, Project administration., (Copyright © 2020 Elsevier B.V. All rights reserved.)