Search

Biochars have drawn wide attention as adsorbents, carbon sequesters and soil re-mediators. However, these substances are ambiguous regarding their effects on the motility, phenotypic changes and potential adaptative mechanisms of soil organisms. This study investigated how peanut shell biochar (PBC) affects the C. elegans model via a one-choice selection test and RNA-seq analysis. The results showed that C. elegans were able to select either PBC or a water control, and a clear preference for PBC was observed after 48 h of exposure, with the chemotaxis index (CI) reaching approximately 1.0. The nematode preferences for PBC vs sterile PBC/graphite were not significant, which demonstrated that initial microorganisms and appearances were not the reasons for the worms' selection, but the selection behaviour was instead determined by volatile odours. The treatments also showed that biochar amendment significantly decreased the body length, brood size and superoxide dismutase (SOD) activity of C. elegans to 960.20 ± 15.23 μm, 173.22 ± 4.56, 165.81 ± 3.82 U/mL SOD, respectively. Then, a possible molecular mechanism of PBC-induced developmental and reproductive effects on C. elegans was explored. Differential gene expression analysis was performed, and 1625 genes (1425 up- and 225 downregulated genes) were regulated in response to PBC treatment. The top 20 regulated genes were col genes (col-129 ; col-81 ; col-139 ; col-71), bli-6 , perm-4 and his-24 , which indicated that cuticle collagen synthesis, eggshell formation and/or heterochromatin in postembryonic growth may be disrupted following exposure to PBC. Therefore, our study suggested that quality standards be used to test nematode preferences and responses to biochar amendment, with the aim of safe application in soils, seedling substrates or fertilizers. Unlabelled Image • C. elegans has a clear migration to PBC by volatile odours • C. elegans became shorter and had fewer off-springs by exposed to PBC • 84.65% DGEs genes were upregulated in response to PBC. • Regulating col- , bli-6 , perm-4 and his-24 genes can affect the growth and reproduction of C. elegans. [ABSTRACT FROM AUTHOR]

Diesel fuel (DF)-contamination remediation has aroused increasing concern in environmental field. In our work, nano sponge was modified by silylation of amino silicon oil (ASO) and aminopropyltriethoxysilane (APTES) to obtain hydrophobic nano sponge (SPAA). SPAA possessed a micro/nano network structure and could efficiently adsorb DF through hydrophobic polysiloxane group. The results revealed that SPAA could effectively remove DF and control its migration in water and soil under various conditions. Importantly, SPAA could inhibit the harmful effect of DF on the growth of plants, earthworms, and fish. Therefore, this work provides a promising and low-cost approach for removal of DF from water and soil, which might have a potential application value. Unlabelled Image • A hydrophobic nano sponge (SPAA) was fabricated to adsorb diesel fuel (DF). • SPAA could effectively control DF migration and remove DF from water and soil. • SPAA displayed a relatively good reuse performance. • DF could be conveniently separated from SPAA. • SPAA was stable against coexisting ions and temperature. [ABSTRACT FROM AUTHOR]

In this work, biochar based hydrogel microspheres were fabricated successfully to develop pH and ion strength dual-stimuli responsively controlled-release system for hydrophilic pesticide. Herein, gentian violet (GV) was selected as model hydrophilic pesticide. Taking advantage of the cross-linking reaction, GV was incorporated into biochar and the 3D network-structured hydrogel, guaranteeing a satisfying encapsulation efficiency and sustained release of pesticide. The leaching behavior of pesticide in simulated soil column at different pHs and ion strength was in accordance with the corresponding release performance, and bulk of pesticide was retarded on the surface. In addition, the pesticide carrier had nearly no toxic effect on the cell proliferation and zebrafish embryo, displaying a good biosafety. The work provides a promising strategy with a low-cost and simple procedure that could regulate pesticide release behavior, decrease leaching loss, and improve the utilization efficiency of pesticide. A pH and ion strength responsive system was fabricated to control the release, decrease leaching loss, and improve utilization efficiency of hydrophilic pesticide. Unlabelled Image • A pH and ion strength dual-stimuli responsive system was fabricated. • The system exhibited a controlled release performance. • The system could decrease the leaching loss of hydrophilic pesticide. • The pesticide carrier had little toxic effect on cell proliferation and zebrafish embryo. [ABSTRACT FROM AUTHOR]