1. Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors.
- Author
-
Padgett RA, Konarska MM, Grabowski PJ, Hardy SF, and Sharp PA
- Subjects
- Adenoviruses, Human metabolism, Base Sequence, Chemical Phenomena, Chemistry, Nucleic Acid Conformation, Nucleic Acid Precursors analysis, Oligoribonucleotides metabolism, Phosphates metabolism, RNA analysis, RNA Precursors, RNA, Messenger analysis, RNA, Viral analysis, Nucleic Acid Precursors metabolism, RNA metabolism, RNA Splicing, RNA, Messenger metabolism, RNA, Viral metabolism
- Abstract
The splicing of messenger RNA precursors in vitro proceeds through an intermediate that has the 5' end of the intervening sequence joined to a site near the 3' splice site. This lariat structure, which has been characterized for an adenovirus 2 major late transcript, has a branch point, with 2'-5' and 3'-5' phosphodiester bonds emanating from a single adenosine residue. The excised intervening sequence retains the branch site and terminates in a guanosine residue with a 3' hydroxyl group. The phosphate group at the splice junction between the two exons originates from the 3' splice site at the precursor.
- Published
- 1984
- Full Text
- View/download PDF