1. Visualizing antigen-specific and infected cells in situ predicts outcomes in early viral infection.
- Author
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Li Q, Skinner PJ, Ha SJ, Duan L, Mattila TL, Hage A, White C, Barber DL, O'Mara L, Southern PJ, Reilly CS, Carlis JV, Miller CJ, Ahmed R, and Haase AT
- Subjects
- Animals, Arenaviridae Infections virology, Cell Count, Cervix Uteri immunology, Cervix Uteri virology, Female, In Situ Hybridization, Lymph Nodes immunology, Lymph Nodes virology, Lymphoid Tissue immunology, Lymphoid Tissue virology, Macaca mulatta, Mice, RNA, Viral analysis, Simian Acquired Immunodeficiency Syndrome virology, Simian Immunodeficiency Virus physiology, Spleen immunology, Spleen virology, Staining and Labeling, Time Factors, Vagina immunology, Vagina virology, Virus Replication, Arenaviridae Infections immunology, Lymphocytic choriomeningitis virus immunology, Simian Acquired Immunodeficiency Syndrome immunology, Simian Immunodeficiency Virus immunology, T-Lymphocytes, Cytotoxic immunology
- Abstract
In the early stages of viral infection, outcomes depend on a race between expansion of infection and the immune response generated to contain it. We combined in situ tetramer staining with in situ hybridization to visualize, map, and quantify relationships between immune effector cells and their targets in tissues. In simian immunodeficiency virus infections in macaques and lymphocytic choriomeningitis virus infections in mice, the magnitude and timing of the establishment of an excess of effector cells versus targets were found to correlate with the extent of control and the infection outcome (i.e., control and clearance versus partial or poor control and persistent infection). This method highlights the importance of the location, timing, and magnitude of the immune response needed for a vaccine to be effective against agents of persistent infection, such as HIV-1.
- Published
- 2009
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