1. Requirement for the leukocyte-specific adapter protein SLP-76 for normal T cell development.
- Author
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Clements JL, Yang B, Ross-Barta SE, Eliason SL, Hrstka RF, Williamson RA, and Koretzky GA
- Subjects
- Adaptor Proteins, Signal Transducing, Animals, B-Lymphocytes cytology, B-Lymphocytes immunology, Gene Targeting, Immunoglobulin M blood, Killer Cells, Natural cytology, Lymph Nodes cytology, Lymphocyte Activation, Lymphocyte Count, Macrophages cytology, Mice, Mice, Inbred C57BL, Phosphoproteins genetics, Protein-Tyrosine Kinases metabolism, Receptors, Antigen, T-Cell metabolism, Signal Transduction, Spleen cytology, Thymus Gland cytology, ZAP-70 Protein-Tyrosine Kinase, Leukopoiesis, Phosphoproteins physiology, T-Lymphocytes cytology
- Abstract
The leukocyte-specific adapter molecule SLP-76 (Src homology 2 domain-containing leukocyte protein of 76 kilodaltons) is rapidly phosphorylated on tyrosine residues after receptor ligation in several hematopoietically derived cell types. Mice made deficient for SLP-76 expression contained no peripheral T cells as a result of an early block in thymopoiesis. Macrophage and natural killer cell compartments were intact in SLP-76-deficient mice, despite SLP-76 expression in these lineages in wild-type mice. Thus, the SLP-76 adapter protein is required for normal thymocyte development and plays a crucial role in translating signals mediated by pre-T cell receptors into distal biochemical events.
- Published
- 1998
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