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Internal moisture generation (IMG), or moisture generated by building occupants via activities such as respiration, cooking, bathing, and cleaning, is a critical input required for design, analysis, and simulation of building enclosure and HVAC systems. Based on previously published values, ASHRAE Standard 160-2021 provides guidance for estimating IMG rates for moisture control design analysis, which is based on occupied home datasets collected in the 1980s and 1990s. Residential energy use simulation software also utilizes estimates for IMG for energy rating, energy analysis, and code compliance calculations, as specified in ANSI/RESNET/ICC Standard 301. Data quantifying IMG rates in newer homes are useful in determining the continued relevance of current design and simulation guidance, and whether the guidance represents conditions found in new housing stock. ASHRAE Research Project 1844, conducted by the Florida Solar Energy Center (FSEC), a research institute of the University of Central Florida, estimated IMG rates in newer occupied homes built since 2013 in the southeastern United States using a moisture balance model approach. The project obtained occupied home data in cooperation with a U.S. Department of Energy Building America research project to characterize indoor air quality (IAQ) in newer U.S. homes, along with presence, functionality, and occupant use of control measures. Full-scale laboratory homes operating with known IMG rates were used to validate a moisture balance model and quantify the accuracy of estimates obtained when the model is applied to data from occupied homes with unknown IMG rates. [ABSTRACT FROM AUTHOR]