1. Very fast CRISPR on demand
- Author
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Taekjip Ha, Yuta Nihongaki, Shiva Razavi, Shuaixin He, Yang Liu, Roger S. Zou, Bin Wu, and Xiaoguang Li
- Subjects
DNA Repair ,Light ,DNA repair ,DNA damage ,010402 general chemistry ,01 natural sciences ,Article ,Histones ,03 medical and health sciences ,chemistry.chemical_compound ,Genome editing ,CRISPR-Associated Protein 9 ,Humans ,CRISPR ,Clustered Regularly Interspaced Short Palindromic Repeats ,DNA Breaks, Double-Stranded ,DNA Cleavage ,Phosphorylation ,030304 developmental biology ,Gene Editing ,chemistry.chemical_classification ,MRE11 Homologue Protein ,0303 health sciences ,DNA ligase ,Multidisciplinary ,Cas9 ,Optical Imaging ,RNA ,0104 chemical sciences ,Cell biology ,HEK293 Cells ,chemistry ,CRISPR-Cas Systems ,Single-Cell Analysis ,DNA - Abstract
Very fast CRISPR on demand Numerous efforts have been made to improve the temporal resolution of CRISPR-Cas9–mediated DNA cleavage to the hour time scale. Liu et al. developed a Cas9 system that achieved genome-editing manipulation at the second time scale (see the Perspective by Medhi and Jasin). Part of the guide RNA is chemically caged, allowing the Cas9-guide RNA complex to bind at a specific genomic locus without cleavage until activation by light. This fast CRISPR system achieves genome editing at high temporal resolution, enabling the study of early molecular events of DNA repair processes. This system also has high spatial resolution at short time scales, allowing editing of one genomic allele while leaving the other unperturbed. Science , this issue p. 1265 ; see also p. 1180
- Published
- 2020
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