1. Toward dynamic structural biology: Two decades of single-molecule Förster resonance energy transfer
- Author
-
Lerner, Eitan, Cordes, Thorben, Ingargiola, Antonino, Alhadid, Yazan, Chung, SangYoon, Michalet, Xavier, and Weiss, Shimon
- Subjects
Biochemistry and Cell Biology ,Chemical Sciences ,Biological Sciences ,Genetics ,Biotechnology ,1.1 Normal biological development and functioning ,Underpinning research ,Affordable and Clean Energy ,Fluorescence Resonance Energy Transfer ,History ,20th Century ,History ,21st Century ,Molecular Biology ,Nucleic Acid Conformation ,Protein Conformation ,Single Molecule Imaging ,General Science & Technology - Abstract
Classical structural biology can only provide static snapshots of biomacromolecules. Single-molecule Förster resonance energy transfer (smFRET) paved the way for studying dynamics in macromolecular structures under biologically relevant conditions. Since its first implementation in 1996, smFRET experiments have confirmed previously hypothesized mechanisms and provided new insights into many fundamental biological processes, such as DNA maintenance and repair, transcription, translation, and membrane transport. We review 22 years of contributions of smFRET to our understanding of basic mechanisms in biochemistry, molecular biology, and structural biology. Additionally, building on current state-of-the-art implementations of smFRET, we highlight possible future directions for smFRET in applications such as biosensing, high-throughput screening, and molecular diagnostics.
- Published
- 2018