1. DNA sequence determination by hybridization: a strategy for efficient large-scale sequencing
- Author
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Drmanac, R., Drmanac, S., Strezoska, Z., Paunesku, T., Labat, I., Zeremski, M., Snoddy, J., Funkhouser, W.K., Koop, B., Hood, L., and Crkvenjakov, R.
- Subjects
Chromosome mapping -- Methods -- Usage ,Nucleic acid hybridization -- Usage -- Methods ,Science and technology ,Usage ,Methods - Abstract
The concept of sequencing by hybridization (SBH) makes use of an array of all possible n-nucleotide oligomers (n-mers) to identify n-mers present in an unknown DNA sequence. Computational approaches can then be used to assemble the complete sequence. As a validation of this concept, the sequences of three DNA fragments, 343 base pairs in length, were determined with octamer oligonucleotides. Possible applications of SBH include physical mapping (ordering) of overlapping DNA clones, sequence checking, DNA fingerprinting comparisons of normal and disease-causing genes, and the identification of DNA fragments with particular sequence motifs in complementary DNA and genomic libraries. The SBH techniques may accelerate the mapping and sequencing phases of the human genome project., The success of the human genome project will depend on whether DNA sequencing approaches can greatly increase through-put [at least 100-fold more than the current value of [10.sup.4] base pairs [...]
- Published
- 1993