1. Monoclonal antibodies for identification of Borrelia afzelii sp. nov. associated with late cutaneous manifestations of Lyme borreliosis.
- Author
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Canica MM, Nato F, du Merle L, Mazie JC, Baranton G, and Postic D
- Subjects
- Acrodermatitis microbiology, Animals, Antibodies, Bacterial, Bacterial Proteins immunology, Bacterial Proteins isolation & purification, Borrelia pathogenicity, Erythema Chronicum Migrans microbiology, Humans, Mice, Species Specificity, Antibodies, Monoclonal, Borrelia immunology, Borrelia isolation & purification, Lyme Disease microbiology, Skin Diseases, Bacterial microbiology
- Abstract
Borrelia isolates associated with Lyme borreliosis were previously divided into 3 genospecies, B. burgdorferi sensu stricto, B. garinii and group VS461, on the basis of DNA homology. B. burgdorferi sensu stricto and B. garinii were identified by monoclonal antibodies (MAbs), H3TS and D6 respectively, but no MAbs were available to identify group VS461. Two MAbs were produced, I 17.3 and J 8.3 which reacted with OspB and OspA proteins, respectively, of strains belonging to group VS461, which should be named B. afzelii sp. nov. 24 strains were assigned to B. afzelii sp. nov., 11 of them being isolated from skin lesions, 6 from acrodermatitis chronica atrophicans (ACA) and 5 from erythema chronicum migrans (ECM). Although quite unknown in the USA, ACA has frequently been reported in northern Europe where B. afzelii sp. nov. is commonly isolated. This study documents the involvement of B. afzelii sp. nov. as a specific aetiological agent of ACA.
- Published
- 1993
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