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Your search keyword '"Rasheed Zafar"' showing total 4 results
Start Over Author "Rasheed Zafar" Journal saudi medical journal
4 results on '"Rasheed Zafar"'

2. Recognition of oxidized albumin and thyroid antigens by psoriasis autoantibodies

Author

Al-Shobaili, Hani A., Ahmed, Ahmed A., and Rasheed, Zafar

Subjects
Adult, Male, endocrine system, Thyroid Hormones, endocrine system diseases, Middle Aged, Autoantigens, Epitopes, Chronic Disease, Humans, Psoriasis, Original Article, Female, Reactive Oxygen Species, Oxidation-Reduction, Serum Albumin, Autoantibodies
Abstract

Objectives: To investigate the role of reactive-oxygen-species (ROS) induced epitopes on human-serum-albumin (HSA) and thyroid antigens in psoriasis autoimmunity. Methods: This study was performed in the College of Medicine, Qassim University, Buraidah, Saudi Arabia between May 2014 and February 2015. The study was designed to explore the role of ROS-induced epitopes in psoriasis autoimmunity. Singlet-oxygen (or ROS)-induced epitopes on protein (ROS-epitopes-albumin) was characterized by in-vitro and in-vivo. Thyroid antigens were prepared from rabbit thyroid, and thyroglobulin was isolated from thyroid extract. Immunocross-reactions of protein-A purified anti-ROS-epitopes-HSA-immunoglobulin G (IgGs) with thyroid antigen, thyroglobulin, and their oxidized forms were determined. Binding characteristics of autoantibodies in chronic plaque psoriasis patients (n=26) against ROS-epitopes-HSA and also with native and oxidized thyroid antigens were screened, and the results were compared with age-matched controls (n=22). Results: The anti-ROS-epitopes-HSA-IgGs showed cross-reactions with thyroid antigen, thyroglobulin and with their oxidized forms. High degree of specific binding by psoriasis IgGs to ROS-epitopes-HSA, ROS-thyroid antigen and ROS-thyroglobulin was observed. Immunoglobulin G from normal-human-controls showed negligible binding with all tested antigens. Moreover, sera from psoriasis patients had higher levels of carbonyl contents compared with control sera. Conclusion: Structural alterations in albumin, thyroid antigens by ROS, generate unique neo-epitopes that might be one of the factors for the induction of autoantibodies in psoriasis.

Published
2015

4. Immunological studies of oxidized superoxide dismutase in patients with systemic lupus erythematosus. Correlation with disease induction and progression.

Author

Al-Shobaili HA and Rasheed Z

Subjects
Adult, Autoantibodies blood, Case-Control Studies, Disease Progression, Female, Humans, Lupus Erythematosus, Systemic blood, Male, Reactive Oxygen Species immunology, Reactive Oxygen Species metabolism, Severity of Illness Index, Superoxide Dismutase metabolism, Lupus Erythematosus, Systemic immunology, Superoxide Dismutase immunology
Abstract

Objective: To investigate the status and contribution of oxidized superoxide dismutase (SOD) in systemic lupus erythematosus (SLE), and to explore whether oxidized-SOD has a role in disease induction and progression., Methods: This study was performed in the College of Medicine, Qassim University, Buraidah, Kingdom of Saudi Arabia between October 2011 and May 2012. The study was designed to explore the role of oxidized-SOD in SLE autoimmunity. The SOD was modified by reactive oxygen species (ROS) and characterized. Binding characteristics of autoantibodies in SLE patients (n=50) with varying levels of disease activity according to the SLE Disease Activity Index (SLEDAI) against ROS-modified-SOD (ROS-SOD) were screened by immunoassays and the results were compared with healthy age-matched controls (n=34)., Results: The ROS caused extensive damage of SOD. Serum analysis showed significantly higher levels of anti-ROS-SOD antibodies in SLE patients compared with controls. Interestingly, not only was there an increased number of subjects positive for anti-ROS-SOD antibodies, but also the levels of these antibodies were significantly higher among SLE patients, whose SLEDAI scores were >/=20. In addition, a significant correlation was observed between the levels of anti-ROS-SOD antibodies and the SLEDAI score (r=0.796)., Conclusion: Our findings show an association between oxidized-SOD and SLE. The stronger response observed in patients with higher SLEDAI scores suggests that oxidized-SOD may be a useful biomarker in evaluating the progression of SLE and in elucidating the mechanisms of disease pathogenesis.

Published
2012

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