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Start Over Author "Irina Grashkina" Journal saratov fall meeting 2018: optical and nano-technologies for biology and medicine
3 results on '"Irina Grashkina"'

3. Amplification of output signal of laser scanning speckle-microscope using gold nanoparticles for detection of Chlamydia trachomatis bacteria. Part 2: experiments

Author

Onega V. Ulianova, Yuriy Saltykov, Sergey S Ulyanov, Lev A. Dykman, Irina Subbotina, Nadejda Filonova, Olga Larionova, Anna Lyapina, Irina Grashkina, Valentina A. Feodorova, and Sergey S. Zaytsev

Subjects
Diffraction, Materials science, Microscope, business.industry, Scattering, Nanoparticle, medicine.disease_cause, law.invention, Speckle pattern, Colloidal gold, law, medicine, Optoelectronics, business, Chlamydia trachomatis, Gaussian beam
Abstract

Method of speckle-microscopy has been adapted to the problem of detection of Chlamydia trachomatis microbial cells in aqueous suspension and fixed on the glass. Combined system “C. trachomatis bacterial cell”-“monoclonal antibody”- “gold nanoparticle” has been used as a complicated scattering element in the case of formation of biospeckles with a small number of scatterers. Optical model of diffraction of strongly focused Gaussian beam on combined scattering system, containing gold nanoparticles, has been suggested. Simulation of speckles, forming with a small number of scattering nanoparticles, has been carried out. It has been demonstrated that the usage of nanoparticles allows to enhance essentially the output signal of speckle-microscope.

Published
2019
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