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Hydraulic parameters affect the behaviour of various ions in soils. The goal of this paper is to get a better understanding of the relationship between physical and chemical properties and soil permeability at the location of the case study soil profile Velika Gorica, based on physical and chemical data. The soil profile is situated in the Eutric Cambisol of the Zagreb aquifer, Croatia. The Zagreb aquifer represents the only source of potable water for inhabitants of the City of Zagreb and the Zagreb County. Based on the data obtained from particle size analysis, soil hydraulic parameters and measured water content, unsaturated hydraulic conductivity values were calculated for the estimation of soil profile permeability. Soil water retention curves and unsaturated hydraulic conductivities are very similar for all depths because soil texture does not change significantly through the depth. Determination of major anions and cations on soil samples was performed using the method of ion chromatography. The results showed a decrease of ions concentrations after a depth of 0.6 m. SAR distribution in the soil profile shows that SAR values are not significantly changing in the soil profile. The highest CEC and EC values are determined in the horizon Bw developed at a depth of 0.6 m which is consistent with the highest SAR value and ion concentrations. All results suggest that physical and chemical properties of the investigated profile are related to soil permeability. [ABSTRACT FROM AUTHOR]