1. Eugenol triggers CD11b+Gr1+myeloid-derived suppressor cell apoptosisviaendogenous apoptosis pathway
- Author
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Hanan Long, Fangli Ma, Minghua Hu, Yuwei Xu, Ning Tao, Ying Ding, Wensheng Zhang, Zecheng Yang, Zhihai Qin, and Yuanyuan Wang
- Subjects
0301 basic medicine ,biology ,medicine.diagnostic_test ,General Chemical Engineering ,Endogeny ,General Chemistry ,Flow cytometry ,Cell biology ,Eugenol ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,Integrin alpha M ,chemistry ,Western blot ,Cell culture ,Apoptosis ,030220 oncology & carcinogenesis ,biology.protein ,medicine ,Myeloid-derived Suppressor Cell - Abstract
To study the effect and underlying molecular mechanism of eugenol on CD11b+Gr1+ myeloid-derived suppressor cells (MDSCs). The effect of eugenol on the inhibition of immortalized MDSC cell line MSC-2 and murine peritoneal macrophages was detected by MTT. Flow cytometry was used to detect the pro-apoptosis effect of eugenol on MDSCs. The expression levels of apoptosis-related proteins were detected by western blot. Eugenol has a selective inhibitory effect on MDSCs in a dose-dependent manner, which activates an endogenous apoptosis pathway, leading to apoptosis. Eugenol promotes the apoptosis of MDSCs via the intrinsic pathway.
- Published
- 2018
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