1. Identificación de bacterias mediante reacción en cadena de polimerasa en recién nacidos pretérmino con ruptura prematura de membranas.
- Author
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Acosta-García, Jorge Mauricio, Floriano-Sánchez, Esaú, and Gómez-Robledo, Hilda Beatriz
- Subjects
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SEPSIS , *POLYMERASE chain reaction , *NEONATAL mortality , *CAUSES of death , *PREMATURE rupture of fetal membranes , *DISEASE prevalence - Abstract
Introduction. Preterm birth is the leading cause of mortality in the neonatal period, rupture of membranes being responsible for 30% of premature births. In most cases of early neonatal sepsis RPM and the microorganism responsible are present in theamniotic fluid, but these are not always identified by standard techniques of culture by what is not known for certain prevalence of agents pathogens involved in this disease. Method. We performed a prospective, observational, descriptive in preterm premature rupture of membranes who are taking birth sample of cord blood and gastric as pirate for detection of bacteria by culture and PCR methods, determination of protein CRP and CBC data were compared with the findings of chorioamnionitis and early neonatal mortality. Results. A total of 60 infants. The mean maternal age was 25.6 years, the RPM was present in 80% of cases in subsequent pregnancies, the average latency be tweed rupture and birth was 59.6 h, the mean for gestational age in new born infants was 33.6 weeks and for the weight of 1,470 g, chorioamnionitis was observed in 15% when the time of rupture was higher at 96 h and funisitis in the 5% breakthrough times greater than120 h, it was possible to identify bacteria in the new born to 5% of cases by both methods (blood culture and PCR), early neonatal mortality occurred in 3%. Conclusion. There was no increased prevalence of early neonatal sepsis to identify bacteria by PCR compared to blood culture. The identification of bacteria at birth did not correlate with the occurrence of early neonatal mortality. [ABSTRACT FROM AUTHOR]
- Published
- 2013