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Introduction: Phenolic compounds, due to their chemical structure, can absorb ultraviolet energy and reduce the formation of free radicals. Aim: To evaluate the photoprotective and antioxidant activity of phenolic compounds from the observation of in vitro results and to verify the importance of the use of biological models in this perspective. Methodology: A search for articles published in the Pubmed database was carried out between 2010 and 2020, which met the objectives of this work, 44 articles were selected. Results: According to the literature, the instrumental methods used to assess independent photoprotective activity, good correlation in vivo, and demonstrating rapid and effective determination of the sun protection factor. In addition to these, biological methods have been provided for the evaluation of aspects not measured by physical-chemical methods, related to DNA damage, resulting from sun exposure. For the evaluation of antioxidant activity, the DPPH radical method was registered in 92.6 % of published studies and it was observed that antioxidants can increase sun protection and also help in the stability of synthetic sunscreens. Conclusion: Phenolic compounds, especially with antioxidant properties, can be used as photoprotective agents in topical formulations to reduce skin damage induced by UV radiation. [ABSTRACT FROM AUTHOR]

The efficient compounds' design taking advantage of the molecule's structural characteristics and efficient search for therapeutic targets has provided effective tools for the research of new treatments when this is focused on disease cellular mechanisms. Phenotypic changes produced by in vitro interaction between molecules and targets can be monitored quantitatively by live cell imaging. To guarantee adequate interaction, it is necessary to consider different crucial elements: 1. Structural characteristics and molecular dynamics of the evaluated compound. 2. Target relevance for the concern physiopathology. However, overview's ignorance of the drug discovery, from structural and cellular problems, has slowed the new treatments research. This literature review presents some important structural aspects for compounds' characterization as therapeutic candidates and experimental approaches for cellular systems development. Subjects discussed are focused on live cell imaging and we also provide relevant examples. Phenotypic monitoring of interactions' produced effects between the chemical candidate and therapeutic target in a cellular system can favor the efficient search of potentially therapeutic molecules. [ABSTRACT FROM AUTHOR]