1. Purificación Parcial de glucosa oxidasa a partir del hongo Penicillium funiculosum para su uso en el diagnóstico.
- Author
-
Del Rivero, Alonso and Maday y Julieta Delfín
- Subjects
- *
FLAVOPROTEINS , *GLUCURONIC acid , *CHROMOGENIC compounds , *GLUCOSE , *CHROMATOGRAPHIC analysis - Abstract
Glucose oxidase is a flavoprotein, which catalyzes the oxidative transformation of D-glucose to glucuronic acid using Oxygen as electron acceptor. This enzyme togheter with Horst radish peroxidase and a suitable chromogen are essential part of a kit for glucose determination in body fluids and secretions. In this paper we describe the purification of glucose oxidase from Penicillium funiculosum for diagnostic purposes. The original extract was ultrafiltered using a 10 000 Da cut-off membrane yielding 90% and 5-fold purity grade. From this preparation a DEAE Cellulose Ionic Exchange Chromatography procedure was applied resulting in two fractions with glucose oxidase activity. A major fraction yielded 72% and 12-fold purity grade. The enzyme was evaluated in terms of sensitivity and factibility when compared with the same analysis with commercial reagents in the glucose determination assays. [ABSTRACT FROM AUTHOR]
- Published
- 2002