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The purpose of the experiment was to determine the distribution of shear stresses in the measurement area of a natural and modified wood sample. Previous wood shear tests conducted on a typical Iosipescu specimen have shown that a complex stress state exists at the bottom of the notch. With transverse loading of the samples, flexure occurs and normal stresses arise from the bending moment and thus fibers are deformed. For the investigations oriented on shear, shear with stretching, and shear with compression, a special specimen was prepared which differed by notch geometry from a typical Iosipescu specimen. A new test machine is described in the article, which is equipped with special specimen holders to perform investigations in complex stress conditions. Crack patterns recorded for natural and modified wood are presented. For all tests, numerical finite element model simulations were performed to obtain stress distributions inside the specimens. The calculated stress distributions were visualized as contour line projections for natural and modified wood. Transverse shear strength values for the modified Iosipescu sample were found to exceed the magnitude of previously published ASTM D1037-87 test results. The test results proved that the strength properties of anisotropic materials in a complex state of stress can be assessed with great accuracy. This is very important in engineering applications.