1. OA021-01. Construction and characterization of replication competent attenuated NYVAC-based vectors as potential HIV vaccines
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Susan A. Holechek, Mark Parrington, Bertram L. Jacobs, Jim Tartaglia, Karen L. Denzler, Karen V. Kibler, William D. Arndt, Giuseppe Pantaleo, Shukmei Wong, and Trung Huynh
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lcsh:Immunologic diseases. Allergy ,biology ,business.industry ,Virulence ,Bioinformatics ,Virology ,In vitro ,Virus ,chemistry.chemical_compound ,Protein structure ,Infectious Diseases ,chemistry ,biology.protein ,Oral Presentation ,Medicine ,Antibody ,Vaccinia ,Signal transduction ,lcsh:RC581-607 ,business ,human activities ,Gene - Abstract
To decrease virulence, we deleted the E3L gene, which is required for interferon-resistance and virulence, and replaced it with a gene from Ambystoma tigrinum virus (ATV, the new virus is NYVAC-C+12-ATV), which restores a single round of replication. Results In vitro characterization of the constructs demonstrates restoration of replication in primary and human cell lines. NYVAC-C+12-ATV leads to induction of pro-inflammatory signal transduction pathways. Pathogenicity studies in newborn mice demonstrate attenuation of 3–5 logs when compared to wt vaccinia virus or to NYCBH.
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