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Methods A retrospective study was conducted in July, 2011 among male and female infants receiving PMTCT intervention for Early Infant Diagnosis in PHC’s at four different LGA’s of Cross River namely: Akamkpa, Calabar South, Odukpani and Biase respectively. Relevant data of the HIV infection status to male and female infants, whose samples were collected and diagnosed using PCR, was obtained from the National PMTCT-EID register. The data was analyzed using Cross-tabulation.