1. TRANSSCLERAL DELIVERY OF Nd
- Author
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Felipe B. Acquesta, Beatriz Sayuri Takahashi, and Francisco Max Damico
- Subjects
medicine.medical_specialty ,Melanoma, Experimental ,Lasers, Solid-State ,Fundus (eye) ,Vascular occlusion ,law.invention ,Ophthalmoscopy ,Mice ,law ,Ophthalmology ,Tumor Cells, Cultured ,medicine ,Adjuvant therapy ,Animals ,Fluorescein Angiography ,Laser Coagulation ,Neovascularization, Pathologic ,medicine.diagnostic_test ,business.industry ,Choroid Neoplasms ,General Medicine ,Laser ,Fluorescein angiography ,eye diseases ,Sclera ,Mice, Inbred C57BL ,medicine.anatomical_structure ,Angiography ,Female ,Rabbits ,sense organs ,medicine.symptom ,business - Abstract
PURPOSE The aims of this study were to determine the scleral attenuation of focused neodymium: yttrium-lanthanum-fluoride laser at 1,047 nm applied transsclerally and whether transscleral delivery can close the vascular supply at the base of experimental choroidal melanoma in rabbits. METHODS Fifty-two New Zealand albino rabbits were included. Scleral laser attenuation was measured across fresh sclera. B16F10 melanomas were established in the subchoroidal space of 49 rabbits. Twenty-one animals were killed immediately after transscleral treatment, 14 were followed for 2 weeks to 4 weeks, and 14 were followed without treatment. Ophthalmoscopy, fundus photographs, and fluorescein angiography were performed before treatment, immediately after, and weekly during the follow-up. Eyes were examined by light microscopy. RESULTS Sclera attenuated laser energy by 31% ± 7%. Immediately after treatment, angiography showed diffuse hypofluorescence in 71% (15 of 21 rabbits). Light microscopy showed vascular occlusion extending at least two thirds of the tumor thickness from the base. Seven of the 14 tumors followed for 15 days ± 8 days were eradicated. There was no correlation between tumor height and eradication. CONCLUSION Rabbit sclera attenuated 31% ± 7% of laser energy. A single transscleral treatment causes tumor vascular closure at the base and may serve as an adjuvant therapy to ensure destruction of deep and intrascleral tumor cells. more...
- Published
- 2014
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