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In this paper, an asymmetric photonic crystal fiber (PCF) working on surface plasmon resonance (SPR) has been proposed and demonstrated using the wavelength interrogation method and lower birefringence peak method. The proposed sensor contains a D-shaped analyte channel that can detect unknown analytes within the sensing range of 1.42–1.47 refractive index units (RIU) of the analytes. The structural asymmetry induces orthogonal x and y polarization modes. The numerical investigations with the finite element method (FEM) reveal that the sensor has a maximum wavelength sensitivity of 80,000 nm/RIU with a sensor resolution of 1.25 × 10−6 RIU for the y polarization mode and the maximum figure of merit (FOM) is found to be of 370.4 RIU−1. For the x polarization mode, the sensor exhibits a maximum wavelength sensitivity of 53,000 nm/RIU with a resolution of 1.89 × 10−6 RIU, having a maximum figure of merit (FOM) of 351 RIU−1. These results are found by using the wavelength interrogation method via confinement loss. On the other hand, the lower birefringence peak method-based analysis reveals a maximum wavelength sensitivity of 50,000 nm/RIU with a resolution of 2 × 10−6 RIU. As such, it is highly suitable for organic chemical detections and medical diagnostics. In addition, this paper studies the fabrication tolerance on the sensor performance.