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Seismic lines—narrow and straight corridors from which overstory has been removed to allow access for oil and gas exploration—are a major human footprint in the boreal forest of western Canada. With slow to minimal recovery of tree cover along these corridors, seismic lines have become a persistent landscape feature affecting connectivity and habitat quality in forested ecosystems, particularly in wetland areas. Soil mounding is a common ground preparation treatment widely applied along seismic lines, with the expectation that it will enhance tree seedling establishment and improve the return of tree cover to disturbed areas. However, much is still unknown about environmental responses following treatment application. In this study, we compared the ground microtopography in treated and untreated seismic lines, as well as the relative elevation between treated and untreated seismic lines with their adjacent treed peatland. The ground elevation in both treated and untreated sites was significantly lower on seismic lines relative to their adjacent treed peatland, with a greater elevation difference in treated areas. Likewise, ground microtopography was orders of magnitude higher along treated areas compared to the natural variation in the adjacent treed peatland. Given the important changes in relative elevation and topography following treatment application, our results suggest the potential for eventual treatment success may be more unpredictable than expected; this may have critical consequences for other ecological properties beyond the restoration goal of tree establishment and return of tree cover. [ABSTRACT FROM AUTHOR]