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Effective methods for graphite felt (GF) treatment based on Fenton's reagent treatment and thermal modification have been used to improve microbial fuel cell (MFC) performance. The effects of the HO content in Fenton's reagent on the performances of MFCs with two different GFs (GFD and ACN-211) have been investigated. For GFD, a maximum performance of 190 mW/m was achieved with HO volume of 100 ml, while for ACN-211, the maximum performance of 450 mW/m is reached with HO volume of 150 ml. MFCs with both treated and untreated ACN-211 consistently showed higher power densities and greater durabilities than those with treated and untreated GFD. The degree of fouling on the surface of ACN-211 was much less than that on the surface of GFD. The higher surface area and better anti-fouling performance of ACN-211 are responsible for its relatively high power density during long-term operation. Thermal modification of ACN-211 was performed at different temperatures and for different durations, and the effects of treatment conditions on the performances of MFCs were studied. Results showed that an MFC fabricated with ACN-211 treated under 400 °C for 2 h exhibited the highest power density, with a maximum value of 470 mW/m, which is higher than that of an MFC fabricated with ACN-211 treated by Fenton's reagent. A Coulombic efficiency of 35% and an energy efficiency of 10% were achieved for an MFC fabricated with ACN-211. [ABSTRACT FROM AUTHOR]