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This research was carried out to selection of desirable cultivar for organic cultivation of carrot in Korea. The assay of disease resistance, insect tolerance and rate of bolting were investigated on 32 cultivars of genetic resources of carrot. This experiment was carried out at experimental field and greenhouse of Gangneung- Wonju National University. ``PI 223360`` was resistant against powdery mildew. Ten cultivars including ``Oxheart Carrot Heirloom`` were moderately resistant, 22 cultivars including ``Long Impeator #58`` were susceptible to powdery mildew. Although there was no resistant cultivar against black rot and leaf blight, our results showed that several cultivars have moderate resistance. Thirteen cultivars including ``Oxheart Carrot Heirloom`` were having insect (Erythroplusia pyropia) tolerance and ``SA 102`` and ``Scarlet Keeper Carrot Rare`` were susceptible. According to the investigation of bolting, 6 cultivars including ``Hongsim Ouchon Carrot`` were early bolting and 4 cultivars including ``Japanese Imperial Long Carrot`` were moderate bolting. Other cultivars were identified as late bolting. From above results, we confirmed that ``Oxheart Carrot Heirloom`` was suitable cultivar for organic cultivation among 32 genetic resources of carrot.