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Start Over Author "William Borges" Journal reproductive toxicology
3 results on '"William Borges"'

1. Melatonin delivery by nanocapsules during in vitro bovine oocyte maturation decreased the reactive oxygen species of oocytes and embryos

Author

Remião, Mariana Härter, Lucas, Caroline Gomes, Domingues, William Borges, Silveira, Tony, Barther, Nathaniele Nebel, Komninou, Eliza Rossi, Basso, Andrea Cristina, Jornada, Denise Soledade, Beck, Ruy Carlos Ruver, Pohlmann, Adriana Raffin, Junior, Antonio Sérgio Varela, Seixas, Fabiana Kömmling, Campos, Vinicius Farias, Guterres, Silvia Stanisçuaski, and Collares, Tiago

Published
2016
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2. Tretinoin-loaded lipid-core nanocapsules decrease reactive oxygen species levels and improve bovine embryonic development during in vitro oocyte maturation

Author

Lucas, Caroline Gomes, Remião, Mariana Härter, Komninou, Eliza Rossi, Domingues, William Borges, Haas, Cristina, Leon, Priscila Marques Moura de, Campos, Vinicius Farias, Ourique, Aline, Guterres, Silvia S., Pohlmann, Adriana R., Basso, Andrea Cristina, Seixas, Fabiana Kömmling, Beck, Ruy Carlos Ruver, and Collares, Tiago

Published
2015
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3. Tretinoin-loaded lipid-core nanocapsules decrease reactive oxygen species levels and improve bovine embryonic development during in vitro oocyte maturation

Author

Vinicius Farias Campos, Andrea Cristina Basso, Tiago Collares, Fabiana Kömmling Seixas, Eliza Rossi Komninou, C. S. Haas, Adriana Raffin Pohlmann, Priscila Marques Moura de Leon, Ruy Carlos Ruver Beck, Caroline G Lucas, William Borges Domingues, Mariana H. Remião, Silvia Stanisçuaski Guterres, and Aline Ferreira Ourique

Subjects
medicine.drug_class, Chemistry, Pharmaceutical, Embryonic Development, Apoptosis, Tretinoin, Biology, Toxicology, Antioxidants, Nanocapsules, Embryo Culture Techniques, Andrology, Pregnancy, medicine, Animals, Retinoid, Blastocyst, Phosphorylation, bcl-2-Associated X Protein, Caspase 7, chemistry.chemical_classification, Drug Carriers, Reactive oxygen species, Dose-Response Relationship, Drug, Caspase 3, business.industry, Embryogenesis, Gene Expression Regulation, Developmental, Fertility Agents, Female, Oocyte, In Vitro Oocyte Maturation Techniques, Biotechnology, Nanomedicine, medicine.anatomical_structure, Shc Signaling Adaptor Proteins, chemistry, embryonic structures, Toxicity, Cattle, Female, Reactive Oxygen Species, business, Signal Transduction, medicine.drug
Abstract

In vitro oocyte maturation (IVM) protocols can be improved by adding chemical supplements to the culture media. Tretinoin is considered an important retinoid in embryonic development and its association with lipid-core nanocapsules (TTN-LNC) represents an innovative way of improving its solubility, and chemical stability, and reducing its toxicity. The effects of supplementing IVM medium with TTN-LNC was evaluated by analyzing production of reactive oxygen species (ROS), S36-phosphorilated-p66Shc levels and caspase activity in early embryonic development, and expression of apoptosis and pluripotency genes in blastocysts. The lowest concentration tested (0.25μM) of TTN-LNC generated higher blastocyst rate, lower ROS production and S36-p66Shc amount. Additionally, expression of BAX and SHC1 were lower in both non-encapsulated tretinoin (TTN) and TTN-LNC-treated groups. Nanoencapsulation allowed the use of smaller concentrations of tretinoin to supplement IVM medium thus reducing toxic effects related with its use, decreasing ROS levels and apoptose frequency, and improving the blastocyst rates.

Published
2015

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