1. Morphine enhances renal cell carcinoma aggressiveness through promotes survivin level
- Author
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Zhongzhong Ren, Shuyong Ma, Peiyan Ding, Wenjun Yan, Dong Wang, Yabing Ma, and Man He
- Subjects
Clear cell renal cell carcinoma ,0301 basic medicine ,Oncology ,renal cell carcinoma ,medicine.medical_specialty ,Survivin ,Apoptosis ,Critical Care and Intensive Care Medicine ,Inhibitor of Apoptosis Proteins ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Renal cell carcinoma ,Cell Line, Tumor ,Laboratory Study ,Internal medicine ,medicine ,Humans ,MTT assay ,Carcinoma, Renal Cell ,Cell Proliferation ,Morphine ,business.industry ,Cell growth ,Cancer ,General Medicine ,medicine.disease ,Analgesics, Opioid ,030104 developmental biology ,Nephrology ,030220 oncology & carcinogenesis ,Cancer research ,business ,Signal Transduction ,medicine.drug - Abstract
Background: Morphine is an opioid analgesic drug often used for pain relief in cancer patients. However, there is growing evidence that morphine may modulate tumor growth, progression and metastasis. Unfortunately, the results obtained by these studies are still contradictory. Methods: In this study, we investigated the effect of morphine in human clear cell renal cell carcinoma 786-O, RLC-310 cells and whether morphine affects on tumor growth in human clear cell renal cell carcinoma 786-O, RLC-310 cells. The cell proliferation was determined by MTT assay, cell proliferation, migration and invasion assays. Immunofluorescence staining and Q-PCR was used to determine the Survivin expression. Results: It was shown that morphine enhances proliferation of 786-O, RLC-310 cells, whereas morphine promoted the growth and aggressive phenotype of 786-O and RLC-310 cells in vitro though Survivin-dependent signaling. Conclusions: Our data showed that morphine promotes RCC growth and increases RCC progression via over-expression of Survivin.
- Published
- 2016