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North American ash species (Fraxinus spp.) are under dire threat from the invasive pest, emerald ash borer (Agrilus planipennis, EAB), and mapping ash trees is of utmost significance for conservation and prevention efforts. We developed remote sensing techniques to identify ash trees at the individual tree level in a mixed-species forest in Maine, USA, using hyperspectral bands combined with derived spectral vegetation indices (SVIs), texture metrics using Random Forest (RF) and Support Vector Machine (SVM) algorithms. The pixel-based SVM-Optimized model proved to be the most accurate classification method with the lowest number of input variables including six Minimum Noise Fraction (MNF)-reduced bands, four SVIs and two texture variables, achieving 78.2% overall accuracy and 81.0% ash Producer's Accuracy. The technique presented in this study could be used to map ash trees throughout Maine and other states with similar forest types for future preservation efforts. [ABSTRACT FROM AUTHOR]