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Using remote-sensing technology to accurately map the composition and distribution of tree species is vital for sustainable forest resource management. Sentinel-2 data with the dense time-series observations enable to identify tree species. However, few studies clarify the differences in classification using Sentinel-2 images in natural forest and planted forest. Two study areas with different forest environments (planted forest and natural forest) were selected to evaluate the potential of Sentinel-2 imagery. Our results show that red-edge band, short-wave infrared (SWIR) band and vegetation indices (VIs), such as Red-Edge Normalized Difference Vegetation Index (ReNDVI), Soil-Adjusted Vegetation Index (SAVI), and Enhanced Vegetation Index (EVI), have important effects on tree species classification, especially in the growing season. By using two machine learning algorithms (support vector machine [SVM] and random forest [RF]), the results show that the classification accuracy for planted forests (91.27% in SVM and 88.35% in RF) significantly exceeds that of natural forests (84.34% in SVM and 81.03% in RF). This accuracy difference may be related to the spatial heterogeneity inside the forest and the surrounding environmental implications. Although the multi-temporal Sentinel-2 images produce satisfactory accuracy for classifying tree species, further research is needed to improve the classification accuracy. [ABSTRACT FROM AUTHOR]