One of the main difficulties in the study of emotion is the induction of a real emotional response by means of artificial techniques. The aim of the current study is to validate the Spanish version of a set of films with the capacity to induce emotions (PIE) under laboratory conditions and to analyze its capacity to provoke differentiated basic emotions. A sample of 127 subjects took part in the study; 57 excerpts of Spanish-dubbed films with capacity in previous studies to induce 7 emotions: anger, fear, sadness, disgust, amusement, tenderness, and neutral emotion were used. Subjective emotional response was measured using the Self-Assessment Manikins and the Discrete Emotions Questionnaire. Films included showed a good capacity to induce positive and negative affects, high levels of emotional activation and variations in the perception of emotional control. They induced basic emotions of amusement and fear in a differentiated way. However, sadness and disgust could not be significantly differentiated from anger; or anger and tenderness from sadness. The PIE could be a useful tool for the experimental research of emotions in Spanish populations.