1. Proteomic analysis of hepatitis B virus-associated hepatocellular carcinoma: Identification of potential tumor markers
- Author
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Sujun Li, Rong Zeng, Yi Hong, Qi-Chang Xia, Hongyang Wang, Danjun Ma, Chen Li, Lei Zhang, Yexiong Tan, Shi-Jian Ding, Xiao-bo Man, Jiarui Wu, Hu Zhou, and Long Li
- Subjects
Adult ,Gene Expression Regulation, Viral ,Male ,Proteomics ,Hepatitis B virus ,Carcinoma, Hepatocellular ,Blotting, Western ,Molecular Sequence Data ,Biology ,medicine.disease_cause ,Biochemistry ,Mass Spectrometry ,Peptide mass fingerprinting ,Cell Line, Tumor ,Proliferating Cell Nuclear Antigen ,medicine ,Biomarkers, Tumor ,Image Processing, Computer-Assisted ,Humans ,Protein Isoforms ,Electrophoresis, Gel, Two-Dimensional ,Amino Acid Sequence ,Molecular Biology ,Peptide sequence ,Gel electrophoresis ,Two-dimensional gel electrophoresis ,Computational Biology ,Middle Aged ,medicine.disease ,Hepatitis B ,Phosphoproteins ,Molecular biology ,Up-Regulation ,Blot ,Gene Expression Regulation, Neoplastic ,Hepatocellular carcinoma ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Proteome ,Microtubule Proteins ,Stathmin ,Female ,Chromatography, Liquid - Abstract
Hepatocellular carcinoma (HCC) is a malignancy of both underdeveloped and developing countries. Proteomes of ten pairs of clinical hepatitis B virus associated HCC tissue samples were obtained by high resolution two-dimensional gel electrophoresis. Comprehensive analyses of proteins associated with B-type HCC were focused on total differentially expressed proteins (> or = two-fold increase or decrease, Student's t-test, p < 0.05) from one pair of samples. Protein identification was done by peptide mass fingerprinting with matrix assisted laser desorption/ionization-time of flight mass spectrometry and liquid chromatography-tandem mass spectrometry. Comparative analyses of proteins associated with B-type HCC included repeat statistics in ten cases. A total of 100 protein spots, corresponding to 80 different gene products, were identified. Proteins whose expression levels were different by more than 2-fold in at least 50% of the cases (five of ten cases) were further analyzed and 45 proteins were selected out as candidates for HCC-associated proteins. Western blotting further validated up-regulated expressions of two candidate proteins in tumor tissues: proliferating cell antigen and stathmin 1. This comprehensive and comparative analyses of proteins associated with B-type HCC could provide useful molecular markers for diagnostics and prognostics and for therapeutic targets. The physiological significance of the differential expressions for several candidate proteins are discussed.
- Published
- 2005