1. Proteomics show antigen presentation processes in human immune cells after AS03-H5N1 vaccination
- Author
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Kristen L. Hoek, Sebastian Joyce, Heather Hill, Laura E. Gordy, Parimal Samir, Xinnan Niu, Travis L. Jensen, Johannes B. Goll, C. Buddy Creech, Kathryn M. Edwards, Allison C. Galassie, Leigh M Howard, Andrew J. Link, and Tara M. Allos
- Subjects
Proteomics ,0301 basic medicine ,Proteome ,Neutrophils ,T-Lymphocytes ,medicine.medical_treatment ,Antigen presentation ,Human leukocyte antigen ,Biochemistry ,Monocytes ,Article ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,Adjuvants, Immunologic ,Antigen ,Influenza, Human ,MHC class I ,medicine ,Humans ,Protein Interaction Maps ,030212 general & internal medicine ,Molecular Biology ,Cells, Cultured ,Antigen Presentation ,B-Lymphocytes ,Influenza A Virus, H5N1 Subtype ,biology ,Antigen processing ,MHC class I antigen ,Killer Cells, Natural ,030104 developmental biology ,Influenza Vaccines ,Immunology ,biology.protein ,Adjuvant - Abstract
Adjuvants enhance immunity elicited by vaccines through mechanisms that are poorly understood. Using a systems biology approach, we investigated temporal protein expression changes in five primary human immune cell populations: neutrophils, monocytes, natural killer cells, T cells, and B cells after administration of either an Adjuvant System 03 adjuvanted or unadjuvanted split-virus H5N1 influenza vaccine. Monocytes demonstrated the strongest differential signal between vaccine groups. On day 3 post-vaccination, several antigen presentation-related pathways, including MHC class I-mediated antigen processing and presentation, were enriched in monocytes and neutrophils and expression of HLA class I proteins was increased in the Adjuvant System 03 group. We identified several protein families whose proteomic responses predicted seroprotective antibody responses (>1:40 hemagglutination inhibition titer), including inflammation and oxidative stress proteins at day 1 as well as immunoproteasome subunit (PSME1 and PSME2) and HLA class I proteins at day 3 in monocytes. While comparison between temporal proteomic and transcriptomic results showed little overlap overall, enrichment of the MHC class I antigen processing and presentation pathway in monocytes and neutrophils was confirmed by both approaches.
- Published
- 2017