3 results on '"Bultelle, Florence"'
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2. 2-DE Mapping of the Blue Mussel Gill Proteome: The Usual Suspects Revisited
- Author
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Le Foll, Frank, Rocher, Béatrice, Bultelle, Florence, Chan, Philippe, Foll, Frank, Letendre, Julie, Monsinjon, Tiphaine, Olivier, Stéphanie, Péden, Romain, Poret, Agnès, Vaudry, David, Knigge, Thomas, Stress Environnementaux et BIOsurveillance des milieux aquatiques (SEBIO), Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de l'Environnement Industriel et des Risques (INERIS), Neuroendocrinologie cellulaire et moléculaire, Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Différenciation et communication neuronale et neuroendocrine (DC2N), Sciences Appliquées à L'Environnement (SCALE), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS), Normandie Université (NU), Plate-forme de Protéomique PISSARO, Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), International Associated laboratory Samuel de Champlain, Institut National de la Recherche Scientifique [Québec] (INRS)-Université de Rouen Normandie (UNIROUEN), LE FOLL, Frank, Institut National de l'Environnement Industriel et des Risques (INERIS)-Université de Reims Champagne-Ardenne (URCA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-High-tech Research Infrastructures for Life Sciences (HeRacLeS), Normandie Université (NU)-Normandie Université (NU)-Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), and Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
animal structures ,[SDE.MCG]Environmental Sciences/Global Changes ,Clinical Biochemistry ,lcsh:QR1-502 ,Environmental pollution ,[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] ,[SDV.BC.IC] Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] ,Proteomics ,[SDV.IMM.II]Life Sciences [q-bio]/Immunology/Innate immunity ,Biochemistry ,Article ,lcsh:Microbiology ,ecotoxicology ,chemistry.chemical_compound ,[SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems ,Structural Biology ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB] ,[SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC] ,post-translational modifications ,14. Life underwater ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,Biomarker discovery ,[SDV.IMM.II] Life Sciences [q-bio]/Immunology/Innate immunity ,Molecular Biology ,ComputingMilieux_MISCELLANEOUS ,mass spectrometry ,[SDV.TOX.ECO] Life Sciences [q-bio]/Toxicology/Ecotoxicology ,biology ,Ecology ,fungi ,proteoforms ,2-DE ,biology.organism_classification ,Mytilus ,bivalve mollusc ,environmental stress ,Proteasome ,chemistry ,Proteome ,[SDV.TOX.ECO]Life Sciences [q-bio]/Toxicology/Ecotoxicology ,Xenobiotic ,Blue mussel - Abstract
The Blue Mussel (Mytilus edulis, L. 1758) is an ecologically important and commercially relevant bivalve. Because of its ability to bioconcentrate xenobiotics, it is also a widespread sentinel species for environmental pollution, which has been used in ecotoxicological studies for biomarker assessment. Consequently, numerous proteomics studies have been carried out in various research contexts using mussels of the genus Mytilus, which intended to improve our understanding of complex physiological processes related to reproduction, adaptation to physical stressors or shell formation and for biomarker discovery. Differential-display 2-DE proteomics relies on an extensive knowledge of the proteome with as many proteoforms identified as possible. To this end, extensive characterization of proteins was performed in order to increase our knowledge of the Mytilus gill proteome. On average, 700 spots were detected on 2-DE gels by colloidal blue staining, of which 122 different, non-redundant proteins comprising 203 proteoforms could be identified by tandem mass spectrometry. These proteins could be attributed to four major categories: (i) “metabolism”, including antioxidant defence and degradation of xenobiotics, (ii) “genetic information processing”, comprising transcription and translation as well as folding, sorting, repair and degradation, (iii) “cellular processes”, such as cell motility, transport and catabolism, (iv) “environmental information processing”, including signal transduction and signalling molecules and interaction. The role of cytoskeleton proteins, energetic metabolism, chaperones/stress proteins, protein trafficking and the proteasome are discussed in the light of the exigencies of the intertidal environment, leading to an enhanced stress response, as well as the structural and physiological particularities of the bivalve gill tissue.
- Published
- 2015
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3. 2-DE Mapping of the Blue Mussel Gill Proteome: The Usual Suspects Revisited
- Author
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Rocher, Béatrice, primary, Bultelle, Florence, additional, Chan, Philippe, additional, Foll, Frank, additional, Letendre, Julie, additional, Monsinjon, Tiphaine, additional, Olivier, Stéphanie, additional, Péden, Romain, additional, Poret, Agnès, additional, Vaudry, David, additional, and Knigge, Thomas, additional
- Published
- 2015
- Full Text
- View/download PDF
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