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Objective: To verify whether the selection of different high-fidelity DNA polymerases has an impact on the results of nanopore sequencing of SARS-CoV-2 with ARTIC-nCoV network workflow. Methods: Multiplex PCR amplification of ARTIC-nCoV network workflow were performed on nucleic acid samples of two confirmed COVID-19 patients with KAPA HiFi HotStart ReadyMix, PrimeSTAR GXL DNA Polymerase or NEBNext High-Fidelity 2X PCR Master Mix. The amplicons were sequenced on Nanopore MinION platform, then were analyzed. Results: Under same conditions, different high-fidelity DNA polymerases lead to different results of quality inspection and sequencing quality. NEBNext High-Fidelity 2X PCR Master Mix is the best among the three enzymes in coverage and sequencing depth. Conclusion: NEBNext High-Fidelity 2X PCR Master Mix has better effect in nanopore sequencing with ARTIC-nCoV network workflow. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To evaluate the surgical efficacy of primary retroperitoneal extra-adrenal paraganglioma and analyze its risk factors for survival and recurrence. Methods: From January 1985 to January 2015, 91 patients diagnosed with primary retroperitoneal extra-adrenal paraganglioma were treated in our hospital. And their clinical data were retrospectively analyzed to investigate the related risk factors of prognosis of this disease. Results: The overall1,3,5-year survival rate of benign primary retroperitoneal extra-adrenal paraganglioma was 98.5 % and the recurrence rate was 2 %, 4.5 %, 16 %. Univariate analysis revealed the modus operation of tumor resection were associated with recurrence rate (X2=10.368, P=0.01), the recurrence rate was high for the patients with incomplete resection. The diameter and location of tumor, age, gender had no obvious association with recurrence rate (P>0.05). The overall 1,3,5-year survival rate and recurrence rate of primary retroperitoneal extra-adrenal maligant paraganglioma was 78.5 %, 50 %, 41.5 and 71 %, 85 %, 100 %, respectively. Univariate analysis revealed, tumor grade was associated with prognosis (X2=5.536, P=0.019) and recurrence (X2=5.734, P=0.017) of the primary retroperitoneal extra-adrenal maligant paraganglioma, the tumor grade was lower, the mortality and recurrence rate was higher; the distant metastasis was related to recurrence (X2=4. 067, P=0.044), the patients with distant metastasis had higher recurrence. Conclusions: The prognosis is good in patients with benign retroperitoneal paraganglioma, and the complete surgical treatment is the main treatment method, and the modus operation of tumor resection is associated with recurrence rate; while the prognosis is poor in primary retroperitoneal extra-adrenal maligant paraganglioma, and tumor grade is associated with prognosis and recurrence, and the distant metastasis is related to recurrence. [ABSTRACT FROM AUTHOR]