Search

Objective: To study the chemical constituents of lignans and flavonoids from the Mongolian medicinal material Clematis aethusifolia Turcz., and to explore the basis of its active substances. Methods: The whole plants of C. aethusifolia was extracted by 75% ethanol. The extract was concentrated in vacuo to remove alcohol and suspended in water. And then, it was partitioned with petroleum ether, ethyl acetate, and n-butanol, respectively. The n-butanol extract was isolated and purified by various column chromatography techniques. The structures of the purified compounds were identified by MS (Mass spectrometry), 1H and 13C NMR (Nuclear magnetic resonance) spectroscopy. Results: Three lignans and three flavonoids were identified from C. aethusifolia. Their chemical structures were elucidated as (+) -syringaresinol-4'-O-茁-D-glucopyranoside (1), (+)-pinoresionol-4'-O-茁-D-glucopyranoside (2), (7R,8S,7'R,8'S,7'S,8'R)-4'-guaiacylglyceryl-medioresinol-4-O-茁-D-glucopyranoside (3), quercetin (4), quercetin 3-O-茁-D-rutinoside (5), and quercetin-7-O-茁-D-glucopyranoside (6), respectively. Conclusion: Compounds 3 and 6 were identified for the first time from C. aethusifolia, and compound 3 was discovered firstly from the genus of Clematis. [ABSTRACT FROM AUTHOR]