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In this study, chemical composition determination and in vitro antimicrobial effects of essential oils of Juglans regia plant grown under Aydın ecological conditions were evaluated by using disc diffusion methods. Extractions were carried out with clevenger apparatus and essential oil compositions were determined by Gas Chromatography-Mass Spectrometry (GC-MS). Microorganisms used for the antimicrobial studies were Salmonella enterica serovar typhimirium ATCC 14028 and Staphylococcus aureus subsp. aureus ATCC 25923, Yersinia pseudotuberculosis ATCC 911 and Bacillus cereus 702 ROMA, Enterobacter aerogenes CCM 2531, Bacillus subtilis IMG 22 and Proteus vulgaris FMC. As a results of, essential oils obtained from the Juglans regia leaves plant were detected to contain 9-octadecene (17.71%), hexadecanoic acid (11.32%) and, cembrene (9.49%) at most. For the in vitro antimicrobial activity determination of essential oils, disc diffusion methods were used in our study. Furthermore, 6-17 mm zone diameters were detected in disc diffusion methods respectively. The highest resistance zone were against P. vulgaris and Staphylococcus aureus subsp. aureus with 17 mm diameter while the least resistance zone was detected against Y. pseudotuberculosis, 6 mm diameter. As a result, it is thought that the essential oil content differs in quantity and composition from the past studies, due to the different geographical and environmental effects of the plant. [ABSTRACT FROM AUTHOR]